Tritiated thymidine autoradiography in the regenerating liver of the dog

Embolization of Portal Vein Branches Induces Hepatocyte Replication in Swine: A Potential Step in Hepatic Gene Therapy

“…This specimen showed essentially no bromodeoxyuridine incorporation (Fig 3, C). The lack of bromodeoxyuridine incorporation agrees well with results of other studies (18)(19)(20), which have shown that, under normal conditions, fewer than 0.1% of hepatocytes are replicating.…”

“…Sigel et al (20) studied hepatocyte regeneration in dogs after a 70% partial hepatectomy by analyzing tritiated thymidine incorporation. They found that the percentage of nuclei containing the radiolabel increased to its maximum of 1.5% on day 4 and decreased to 0.6% on day 7 and 0.5% on day 12.…”

“…Nearly all retroviral vectors require cell division for integration (16,17). Because hepatocytes are normally quiescent (18)(19)(20), the protocols for retroviral vectormediated gene therapy have included steps to induce hepatocyte replication. An ex vivo approach (21,22) involved a partial hepatectomy, isolation of hepatocytes, in vitro transduction of replicating cells, and introduction of the genetically modified cells into the portal vein.…”

CC Bond Insertion of a Complexed Phosphinidene into 1,6‐Methano[10]annulene

“…Duncan et al 30 studied liver regeneration in a porcine model that mirrored clinical PVE; hepatocyte replication peaked on day 7 (approximately 14 per cent of hepatocytes) and returned to baseline by day 12. This peak was later than that noted after 70 per cent hepatectomy in a large animal model, in which the peak mitotic index occurred on days 3–431, 32. The relatively low hepatocyte replication index observed in clinical studies compared with experimental observations probably reflects the long interval between PVE and surgery and the fact that PVE is a weaker stimulus than hepatectomy for liver regeneration.…”