Triterpenoids from Vitellaria paradoxa Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages

Sirignano, Carmina; Nadembega, Pascal; Poli, Ferruccio; Romano, Barbara; Lucariello, Giuseppe; Rigano, Daniela; Taglialatela‐Scafati, Orazio

doi:10.3390/plants10051006

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…Similarly, the hexane stem-bark extract was subjected to column chromatography on silica to afford three compounds, including 11-hydroxy β-amyrin cinnamate (1) (Sirignano et al, 2021), α-amyrin cinnamate (2) (Miranda et al, 2000), and sitosterol cinnamate (3) (Buxton et al, 2020) (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Activity of Chemical Constituents from Vitellaria paradoxa Gaertn. (Sapotaceae) Against Pathogens Associated with Respiratory Tract Infections

Ojo,

Mmutlane,

Ndinteh

2024

Preprint

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Vitellaria paradoxa Gaertn. is a commonly used medicinal plant for the treatment of tuberculosis (TB) and other respiratory infections in Africa. Therefore, its phytochemicals can be explored in rational drug discovery, development, and design against respiratory-tract infections. The aim of this study is to identify chemical composition of V. paradoxa hexane stem-bark extract, and further isolate possible antimicrobial agents from its crude extract. 48 phytochemicals, including1-heptacosanol, 2-nonenal, (E)-, and hexadecanoic acid, methyl ester were identified using GC-MS. Column chromatography led to the isolation of three compounds, including 11-hydroxy β-amyrin cinnamate (1), α-amyrin cinnamate (2), and sitosterol cinnamate (3). All the compounds showed activity against all the tested pathogens, with compound (3) showed better activity against all bacterial strains, with minimum inhibitory concentrations (MICs) varying from 0.0625 to 0.25 mg/ml. Compounds (1) and (2) showed no cytotoxicity against the cervical cancer cell line (HeLa) (IC50; 82.53 ± 4.07 and 77.82 ± 10.5 respectively). To our knowledge, compounds 2 and 3 are reported here from the stem-bark part of the plant for the first time. Similarly, inhibitory activities of compounds 1–3 against the tested strains are studied here for the first time. These results provide in part scientific justification for the traditional uses of V. paradoxa for treating respiratory tract infections, including tuberculosis in Africa.

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Section: Resultsmentioning

confidence: 99%

Inhibitory Activity of Chemical Constituents from Vitellaria paradoxa Gaertn. (Sapotaceae) Against Pathogens Associated with Respiratory Tract Infections

Ojo,

Mmutlane,

Ndinteh

2024

Preprint

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“…Previous studies reported that Vitellaria paradoxa seeds, leave, bark, and root extracts are composed of several classes of compounds according to extraction solvent [ 30 – 33 ]. A previous study reported that bark of V. paradoxa ethyl acetate extract afforded ten pure triterpenoids including the previously undescribed natural products ursaldehyde cinnamate and 11-hydroxy-β-amyrin cinnamate [ 32 ]. Additionally, a study showed that shea nut extracts are composed of triterpene alcohol fractions, such as α-amyrin, β-amyrin, lupeol, and butyrospermol [ 16 , 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro trypanocidal activity of extracts and compounds isolated from Vitellaria paradoxa

Bairy,

Ozzin-Kholy Zolipou,

Nzoumbou-Boko

2023

BMC Complement Med Ther

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Background Vitellaria paradoxa is used in traditional medicine for the treatment of various diseases in tropical countries; however, nothing is known about its anti-trypanosomal activity. Human African trypanosomiasis is a neglected tropical disease of Sub-Saharan Africa’s poorest rural regions, and the efficacy of its treatment remains a challenge. This study investigates the as-yet-unknown trypanocidal activity of this plant. Methods V. paradoxa, commonly known as shea tree, was selected for study based on an ethnobotanical investigation. Ultrasonicated extracts from bark and seeds were successively treated with ethyl acetate and water. Column chromatography, NMR spectroscopy and mass spectrometry were used to identify isolated compounds. Purified trypanosomes (Trypanosoma brucei brucei) were incubated with serial dilutions of the extracts and isolated compounds at 37 °C in 5% CO2 for 24 h. Parasite viability was evaluated under a microscope. Results The ethyl acetate extracts of the bark showed the higher in vitro trypanocidal activity against T. brucei brucei with median inhibitory concentration (IC50) of 3.25 µg/mL. However, the triterpene 1α,2β,3β,19α-tretrahydroxyurs-12-en-28-oic acid and the pentadecanoic acid isolated from the ethyl acetate extract of the seeds showed in vitro trypanocidal activity with IC50 of 11.30 and 70.1 µM, respectively. Conclusion The results obtained contribute to the validation of the traditional medicinal use of V. paradoxa. Our results encourage further investigations of this plant, mainly with respect to its in vivo efficacy and toxicity.

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“…This subtropical tree has very large and relatively thick leaves that contains large amounts of secondary metabolites. Among various phytochemicals, TAs were considered as major products based on the previous research on the chemical composition of S. formosum leaves, and many reports on the biological activities of TAs are available [7,9,15,16]. It is well known that secondary metabolites are affected by environmental factors as well as genetic backgrounds.…”

Section: Discussionmentioning

confidence: 99%

“…Many triterpenic acids are proved as strong bioactive compounds and used as pharmaceutical and cosmeceutical ingredients. Asiatic acid, madecassic acid, corosolic acid, and betulinic acids are representative chemicals that are well investigated and industrialized in those ways [16][17][18][19]. In this study, HaCaT cells radiated with UVB were adopted due to the well-established references of inflammatory responses of HaCaT to UVB [12,13].…”

Section: Discussionmentioning

confidence: 99%

Variation of Triterpenic Acids in 12 Wild Syzygium formosum and Anti-Inflammation Activity on Human Keratinocyte HaCaT

Park¹,

Kim

Lee³

et al. 2021

Plants

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Syzygium formosum (Wall.) Masam leaf is known as a Vietnamese traditional herbal medicine used to treat atopic dermatitis and stomach ulcers. Recently, its potent anti-allergic effects were reported with possible active compounds analysis. Here, we collected S. formosum leaves from 12 wild trees and compared compositions of triterpenic acids (TA) with Centella asiatica. Anti-inflammatory activities of S. formosum leaf extract (SFLE) was compared with C. asiatica extract (CAE) using human keratinocyte, HaCaT. In this study, up to seven TAs were identified in SFLE, while only madecassic and asiatic acids were detected in the CAE. Total TA content varied among SFLE, but asiatic, corosolic, and betulinic acids were the major components. Surprisingly, wild tree sample 12 (S12) contained total TA of 27.2 mg/g dry-leaves that was 5-fold greater than that in the C. asiatica sample, and S4 had the highest content of asiatic acid (12.6 mg/g dry-leaves) that accounted for 50% of the total TA. S4 and S12 showed more than 3-fold higher anti-oxidative power than the CAE. In the UVB irradiation model, S4 and S12 (5 μg/mL) strongly repressed mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) and COX-2, while the CAE at the same condition showed moderate or weak repression. The difference in anti-inflammation effects between the SFLE and the CAE was also confirmed by protein quantifications. Taken together, SFLE has great potentials as a new cosmeceutical ingredient with a higher amount of skin-active phytochemicals.

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Triterpenoids from Vitellaria paradoxa Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages

Cited by 5 publications

References 21 publications

Inhibitory Activity of Chemical Constituents from Vitellaria paradoxa Gaertn. (Sapotaceae) Against Pathogens Associated with Respiratory Tract Infections

Inhibitory Activity of Chemical Constituents from Vitellaria paradoxa Gaertn. (Sapotaceae) Against Pathogens Associated with Respiratory Tract Infections

In vitro trypanocidal activity of extracts and compounds isolated from Vitellaria paradoxa

Variation of Triterpenic Acids in 12 Wild Syzygium formosum and Anti-Inflammation Activity on Human Keratinocyte HaCaT

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