Triterpenoids from the roots of Hemidesmus indicus

Padhy, Sachidananda; Mahato, Shashi B.; Dutta, N.L.

doi:10.1016/s0031-9422(00)84656-7

Cited by 23 publications

(8 citation statements)

References 3 publications

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“…Phytochemical tests revealed that fatty acids, steroids and triterpenoids are present in both CHI and ME1 and tannins and glycosides are present in MHI (data not shown). The presence of pregnane oligoglycosides (Deepak et al, 1997), steroids, triterpenoids (Padhy et al, 1973), tannins, two coumarino lignoids, including hemidesmin-1 and hemidesmin-2 in H. indicus root have already been reported.…”

Section: Antienterobacterial Activity Of Extractsmentioning

confidence: 87%

Antienterobacterial activity of Hemidesmus indicus R. Br. root extract

Das

Devaraj

2006

Phytotherapy Research

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The antienterobacterial activity of the chloroform and methanol extracts of Hemidesmus indicus root was demonstrated using a variety of methods and different enterobacterial strains. Although the constituents were similar in the chloroform extract (CHI) and the fatty substance separated (ME1) from the methanol extract (MHI), ME1 was found to be more effective than CHI as evident from the disc diffusion method. ME1 was found to be more active than MHI, followed by CHI. This may be due to the inefficient diffusion of CHI into the medium. In a modified agar well diffusion and swab method the activity of the extract against different strains was observed in a single plate. The extracts inhibited growth in a dose dependent manner; both MHI and CHI were most effective against S. flexneri, least effective against S. dysenterie and moderately effective against the other strains. The presence of antimicrobial trace elements such as copper and zinc, along with other active constituents may contribute to the antienterobacterial activity of Hemidesmus indicus root.

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Section: Antienterobacterial Activity Of Extractsmentioning

confidence: 87%

Antienterobacterial activity of Hemidesmus indicus R. Br. root extract

Das

Devaraj

2006

Phytotherapy Research

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“…The plant is reported for the number of pharmacological properties (Murshed et al 2005;Khandelwal et al 2010). The fragrance of Nannari roots is attributed by the presence of volatile oils, 2-hydroxy-4-methoxy benzaldehyde and ledol (Padhye et al 1973). H. indicus roots are the major storage of active ingredients in patented formulations of the detoxifier herbal drinks and anticigarette (Murty 2008).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: a multi-potent endangered climber

Shekhawat¹,

Manokari²

2016

Ind J Plant Physiol.

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Hemidesmus indicus is an endangered medicinal plant of Peninsular India. A rapid micropropagation method using nodal segments of a 4 year old plant has been achieved in the present study. The nodal explants (2.0-3.0 cm long) were harvested from actively growing shoots of conventionally raised plants and cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 6-benzylaminopurine (BAP). Hundred percent explants responded with 5.2 ± 0.25 cm long multiple shoots (9.0 ± 0.53) from the nodal meristems. The shoots were further multiplied enormously (272 ± 4.12 shoots per explant) by repeated subculture of mother explants and freshly induced shoot clumps on MS medium with the reduced concentrations of BAP (1.0 mg l -1 ), kinetin (Kin; 0.5 mg l -1 ) and indole-3 acetic acid (IAA; 0.1 mg l -1 ) within 4 weeks. Highest root induction (62.0 ± 0.54 roots per shoot) was observed on 1/4th strength MS medium supplemented with 3.0 mg l -1 indole-3 butyric acid (IBA). About 96 % shoots were rooted (45.0 ± 0.48 roots per shoot) by ex vitro methods when the cut ends of the shoots pulse treated with IBA (400 mg l -1 ) for 5 min. The in vitro as well as ex vitro rooted plantlets were acclimatized successfully in the greenhouse. Ex vitro rooted plantlets exhibited higher percentage (98 %) of survival as compared to the in vitro rooted plantlets (91 %) in the field conditions. There were not any observable differences between in vitro propagated and field transplanted plantlets. A comparative foliar micro-morphological study of H. indicus was conducted to understand the micromorphological changes in the plants while shifting from in vitro to the in vivo conditions in terms of variations in stomata, venation pattern and vein density, and trichomes. The aforementioned protocol could be successfully used for the large-scale multiplication and conservation of germplasm of this endangered plant species.

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“…The plates were then maintained at room temperature (26 o ) for 1 hr to allow the diffusion of the solutions into medium and incubated at 37 o C for Xanthomonas citri and Xanthomonas Compstris. Inhibition was recorded by measuring the diameter of the inhibition zone at the end of 24 hr and compared with standard drug [26][27]. As per the observations made, the maximum inhibition effect was observed in Zn-PhImPc with tested organism and the least inhibition effect was observed in Co-PhImPc.…”

Section: Methodmentioning

confidence: 83%