Triterpenoide. XIV. [2,3-<i>d</i>]Isoxazoltriterpenderivate

Gzella, Andrzej; Linkowska, E.; Zaprutko, Lucjusz; Wrzeciono, U.

doi:10.1107/s010827019900013x

Cited by 2 publications

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“…In the last years, many studies were done to identify and to create oleanane‐type triterpenoids, which improve the activities of OA . But chemical modifications of OAs are unspecific and result in unexpected, unwanted products . By means of (highly specific) enzymatic biotransformation the target compound(s) can be produced under physiological conditions without application of toxic compounds.…”

Section: Introductionmentioning

confidence: 99%

Whole‐cell biotransformation of oleanolic acid by free and immobilized cells of Nocardia iowensis: Characterization of new metabolites

Ludwig

Geib

Haas

et al. 2014

Engineering in Life Sciences

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Whole-cell biotransformation of oleanolic acid by free and immobilized cells of Nocardia iowensis: Characterization of new metabolitesIn this study, Nocardia iowensis was used to transform oleanolic acid (OA) into oleanane derivatives. The first derivative, which was found after 24 h of cultivation, was the known and already described OA methyl ester. After 1 week, two other derivatives (oleanonic acid methyl ester and an unknown metabolite) were identified as new products of a biotransformation by N. iowensis. These oleanane metabolites were characterized by HPLC, HPLC-ESI-MS, and HPLC-1 H NMR spectroscopy. The biotransformation was performed by suspended and immobilized cells (ICs) of N. iowensis. Cells immobilized in alginate beads were used in order to prepare a continuous process. The substrate uptake of free and ICs was similar, whereas the peak area of OA methyl ester of the ICs was only about 10% of the native cells. However, the final product (oleanonic acid methyl ester) concentrations were similar in both approaches, whereas the unknown metabolite 3 was only detected transiently in the medium of ICs. Based on these results, a new biosynthetic pathway for the biotechnological production of oleanonic acid methyl ester is proposed.

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Section: Introductionmentioning

confidence: 99%