Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri

Doligalska, Maria; Jóźwicka, Kinga; Kiersnowska, M.; Mroczek, Agnieszka; Pączkowski, Cezary; Janiszowska, Wirginia

doi:10.1016/j.vetpar.2011.01.053

Cited by 29 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The AE and SF demonstrate absence of larvicidal activity, but they showed high efficacy on the egg hatching. Despite the possibility of action of saponins on membranes and proteins present in larvae (Doligalska et al, 2011), the morphological and physiological differences between the stages of the parasite may also have contributed to these results.…”

Section: Larvicidal Activitymentioning

confidence: 97%

In vitro anthelmintic activity of the Zizyphus joazeiro bark against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells

Gomes

Lima

Vaz

et al. 2016

Veterinary Parasitology

View full text Add to dashboard Cite

Section: Larvicidal Activitymentioning

confidence: 97%

In vitro anthelmintic activity of the Zizyphus joazeiro bark against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells

Gomes

Lima

Vaz

et al. 2016

Veterinary Parasitology

View full text Add to dashboard Cite

“…Other specific biological activity of OA molecules is associated with sugar chain linked to the aglycone with glycosidic bond. These complex structures, called saponins, have a better solubility in water and exhibit hemolytic, allelopathic, fungistatic (Szakiel et al 2005), antibacterial, and antiparasitic properties (Szakiel et al 2008;Doligalska et al 2011). Those significant bio-activities and relatively nontoxic nature have made oleanolic acid a highly valuable compound.…”

Section: Introductionmentioning

confidence: 99%

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

2018

View full text Add to dashboard Cite

Triterpenic saponins represented in Calendula officinalis L. by oleanolic acid (OA) glycosides are pentacyclic triterpene compounds with a wide range of biological and medicinal properties. This report demonstrates nitrogen source impact on growth, saponin accumulation, and secretion in hairy root and suspension cultures of marigold. Hairy roots preferred nitrate as a mineral source of nitrogen, but its impact on growth, OA glycosides accumulation, and secretion were line-dependent. The best productivity of OA glycosides was found in CC16 line (74.86 mg flask −1 ) in ½ MS medium modified by 2.5× KNO 3 and ammonium elimination with 2.5 g l −1 peptone. Organic nitrogen source at 27.5-g l −1 impairs the growth rate of hairy roots. Its effect on saponin accumulation and secretion to the surrounding medium depended on line and media composition. Nitrate:ammonium ratio of 4:2 for CC16 resulted in 5.7-fold increment of saponin secretion comparing to the standard medium. Embryo roots, apical bud, and hypocotyls explants were crucial for induction of suspension culture synthesizing saponins; however, effect of mineral form of nitrogen in cultivating medium had to be considered. The highest OA glycosides level (171.97 μg g −1 of dry weight) was recorded in the root derived culture with nitrate as a sole mineral form of nitrogen. Peptone from lactalbumin decidedly inhibited the saponin formation; however, it was essential for culture initiation, proliferation, and organ differentiation.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The viability of Heligmosomoides polygyrus eggs and L3 were tested with the Rho123 fluorescence retention assay, with increased Rho123 fluorescence indicating influx and retention in dead eggs. 13 The results indicated that viability and P-gp function in H. polygyrus eggs and stage L3 were unaffected by the used doses of 10, 50 and 100 nM of XR9576 in vitro. No change was observed in the percentage of dead (trypan blue positive, Rho123 positive) embryos identified in untreated mice and mice given 10, 50 or 100 nM XR9576 in vitro: this value ranged from 8% to 12%.…”

Section: Xr9576 P-gp Inhibitor Does Not Influence the Viability Of mentioning

confidence: 92%

The intestinal nematode inhibits T‐cell reactivity by targeting P‐GP activity

Donskow‐Łysoniewska

Krawczak

Kozłowska

et al. 2017

Parasite Immunology

Self Cite

View full text Add to dashboard Cite

Host immunosuppression occurs during chronic nematode infection, partly due to effector T-cell hyporesponsiveness. The role of P-glycoprotein (P-gp), a member of the ABC transporter family, has been assessed in T-cell activity. This study assesses the possible role of P-gp in T-cell activity during nematode infection. Our findings indicate that blockade of P-gp in vivo increased protection against Heligmosomoides polygyrus nematode infection and was associated with the enhanced T-cell activity. Three P-gp-inhibitors, verapamil (VRP), cyclosporine (CsA) and tariquidar (XR9576), were used to determine the influence of nematode infection on the P-gp function of T cells. The influence of the nematode on the uptake, efflux and kinetics of extrusion in T-cell subsets CD4 and CD8 was assessed by the accumulation of Rho123 dye. The results indicate that H. polygyrus infection contributes to the inhibition of T-cell function by elevating P-gp activity. The blockade of P-gp in the T cells of infected mice led to an impressive increase in T-cell proliferation and IL-4 cytokine release through the upregulation of NF-κB activation. These results provide the first evidence that the P-gp function of T cells is altered during nematode infection to open the way for further studies aiming to explore the role of P-gp in host-parasite interactions.

show abstract

Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri

Cited by 29 publications

References 41 publications

In vitro anthelmintic activity of the Zizyphus joazeiro bark against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells

In vitro anthelmintic activity of the Zizyphus joazeiro bark against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

The intestinal nematode inhibits T‐cell reactivity by targeting P‐GP activity

Contact Info

Product

Resources

About