Triterpenoid saponin-rich fraction of Centella asiatica decreases IL-1β andNF-κB, and augments tissue regeneration and excision wound repair

Abstract

Wound healing is an orderly process driven by numerous cellular mediators that aim to accelerate it. In skin conditions such as leprosy and psoriasis, Centella asiatica, which contains high amounts of triterpenoid saponins (TSs), has been reported to have beneficial effects. This study examined the effects of TS fraction of the herb on tissue regeneration and excision wound repair in rat skin. Briefly, TS fraction was prepared from C. asiatica and applied on the wound area for 7 consecutive days. The IL-1β, NF-κB, and other biochemical parameters were then estimated, followed by examination of inflammatory cell infiltration, epithelialization, angiogenesis, and general morphology of granulation (wound) tissue by histopathology. Treatment decreased the IL-1β and NF-κB, increased epithelialization and collagen synthesis, and decreased matrix metalloproteinases. Asiatic acid, the active ingredient in the fraction, increased cell viability and inhibited nitrite production in macrophage culture. Taken together, TSs augmented wound healing, which could be due to their effect on cellular mediators involved in the process, thus creating a microenvironment that could promote tissue repair and favor tissue remodeling. The fraction, in addition to its effect on excision wounds in rat skin, is speculated to have a similar role in accidental, surgical, and diabetic wounds.

Depigmented Centella asiatica Extraction by Pretreated with Supercritical Carbon Dioxide Fluid for Wound Healing Application

“…This observation might be due to the higher content of asiaticoside, total triterpenoid glycosides, madecassic acid, terminolic acid, asiatic acid and total triterpenes in SCE1 than CV. These active compounds in C. asiatica play an important role in the wound healing process, especially in cell migration [41,43,44] and angiogenesis [7,45]. Moreover, the synergistic effect of these active compounds in C. asiatica could be observed in the wound healing activity.…”

“…In earlier research, MMP-2 and MMP-9 decreased in the wound tissue treated with the triterpenoid saponin-rich fraction of C. asiatica. MMP-2 and MMP-9 regulate the turnover of the matrix proteins by degrading basement membrane proteins, which include collagen IV and V, gelatin, elastin and fibronectin [41]. Other work demonstrated that asiatic acid and triterpene compounds reduced the UVA-induced activation and expression of MMP-2, using HaCaT human keratinocytes as a model cell system [42].…”

Bioassay-Guided Different Extraction Techniques of Carica papaya (Linn.) Leaves on In Vitro Wound-Healing Activities

“…For instance, saponin, which is known as ginsenoside from ginseng, has been proven to accelerate neovascularization in burn wound skin [30], while flavonoids also have been proven to increase the rate of wound contraction and hydroxyproline content, indicating an enhanced synthesis of collagen [31,32]. A triterpenoid saponin-rich fraction from Centella asiatica studied by Mahmood et al, 2016 can decrease Interleukin -1β (IL-1β) and Nuclear factor kappa β (NF-κB), and it augments tissue regeneration and excision wound repair [33].…”

Prangos Platychlaena Boiss Essential Oils: A Novel Study on Its Toxicity, Antibacterial Activity and Chemical Compositions Effect on Burn Rats

“…Up to now, no study on the healing time of wound recovery is available until the preparation of this manuscript. The disruption in normal skin may causes infection and alter homeostasis, the infection of a wound was and still regarded as one of the common diseases in our country (18). The healing of the wound is a complex process in dermal regeneration and the epidermal tissues, because some biological mechanisms such as inflammation, antimicrobial and wound contraction were involved in this process (14).…”