Triterpenoid Glycosides from <i>Medicago sativa</i> as Antifungal Agents against <i>Pyricularia oryzae</i>

Abbruscato, Pamela; Tosi, Solveig; Crispino, Laura; Biazzi, Elisa; Menin, Barbara; Picco, Anna Maria; Pecetti, L.; Avato, Pinarosa; Tava, Aldo

doi:10.1021/jf5049063

Cited by 41 publications

(49 citation statements)

References 38 publications

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“…The obtained results are in agreement with studies by Tava et al [23,27]. Abbruscato et al [30] identified medicagenic acid, zanhic acid, soyasapogenol B, bayogenin, hederagenin, and soyasapogenol A in leaves, while medicagenic acid, hederagenin, soyasapogenol B, bayogenin, and zanhic acid were found in roots ( Figure 2). Other sapogenins in leaves were zanhic acid and soysapogenol B. Hederagenin, bayogenin, and soyasapogenol B were also present in roots [3,23,27].…”

Section: Sapogenins In Morphological Parts Of Medicago Sativasupporting

confidence: 91%

“…In this study, sapogenins were obtained by acid hydrolysis and quantified by GC-FID. Abbruscato et al [30] identified medicagenic acid, zanhic acid, soyasapogenol B, bayogenin, hederagenin, and soyasapogenol A in leaves, while medicagenic acid, hederagenin, soyasapogenol B, bayogenin, and zanhic acid were found in roots ( Figure 2). Concentration of medicagenic acid was the highest in both morphological parts of lucerne.…”

Section: Sapogenins In Morphological Parts Of Medicago Sativamentioning

confidence: 99%

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Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Kiełbasa

Krakowska-Sieprawska

Kowalkowski

et al. 2019

J of Separation Science

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Saponins in plant extracts were indirectly determined by estimation of the content of sapogenins. The first step of determination is extraction with high efficiency. One conventional extraction technique (maceration) and two modern ones (accelerated solvent extraction and supercritical fluid extraction) were compared. Methanol and ethanol were used as solvents or co-solvents. The results were supported by statistical analysis. Saponins were extracted from leaves, roots, and sprouts of Medicago sativa. Acid hydrolysis, purification, and determination by high-performance liquid chromatography with evaporative light scattering detector were used. The content of sapogenins was the highest in the roots. Smaller amounts of sapogenins were found in sprouts and the smallest ones in leaves. The main ingredient was medicagenic acid with mean concentration of 621.8 μg/g in roots, 456.7 μg/g in sprouts, and 471.3 μg/g in leaf extract. The highest content of sapogenins in extract was obtained after maceration with methanol; however, this method is nonselective in relation to biologically active compounds. Due to the possibility of using the obtained extracts with sapogenins in the cosmetic or pharmaceutical industry, the selection of extraction techniques and solvents is a very important aspect. Additionally, the chosen technique should be considered eco-friendly and consistent with the assumptions of "green chemistry." K E Y W O R D Sextraction methods, liquid chromatography, Medigaco sativa, sapogenins, statistical analysis Tenebrio molitor, and Spodoptera littoralis) and nematodes (Meloidogyne incognita, Globodera rostochiensis, and Xiphinema index) [15][16][17][18]. Therefore, plant materials especially rich in saponins are searched for because of J Sep Sci 2020;43:671-680.

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Section: Sapogenins In Morphological Parts Of Medicago Sativasupporting

confidence: 91%

Section: Sapogenins In Morphological Parts Of Medicago Sativamentioning

confidence: 99%

Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Kiełbasa

Krakowska-Sieprawska

Kowalkowski

et al. 2019

J of Separation Science

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“…Guajadial B exhibited antitumor activity in five human cancer cell lines (HCT116, CCRFCEM, DU145, Huh7, and A549) at an IC 50 value of 150 nM [28]. Medicagenic acid has been identified as an antifungal agent against Pyricularia pryzae (minimum inhibitory concentration (MIC) = 0.01 mg/mL and minimum fungicidal concentration (MFC) = 0.03 mg/mL) [29]. In our study, corosolic acid and jacoumaric acid displayed the most interesting antileishmanial activity in the amastigotes stage with respective IC 50 values of 1.01 ± 0.06 µg/mL and 1.32 ± 0.59 µg/mL and an SI above five.…”

Section: Discussionmentioning

confidence: 99%

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

et al. 2019

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With an estimated annual incidence of one million cases, leishmaniasis is one of the top five vector-borne diseases. Currently available medical treatments involve side effects, including toxicity, non-specific targeting, and resistance development. Thus, new antileishmanial chemical entities are of the utmost interest to fight against this disease. The aim of this study was to obtain potential antileishmanial natural products from Psidium guajava leaves using a metabolomic workflow. Several crude extracts from P. guajava leaves harvested from different locations in the Lao People's Democratic Republic (Lao PDR) were profiled by liquid chromatography coupled to high-resolution mass spectrometry, and subsequently evaluated for their antileishmanial activities. The putative active compounds were highlighted by multivariate correlation analysis between the antileishmanial response and chromatographic profiles of P. guajava mixtures. The results showed that the pooled apolar fractions from P. guajava were the most active (IC 50 = 1.96 ± 0.47 µg/mL). Multivariate data analysis of the apolar fractions highlighted a family of triterpenoid compounds, including jacoumaric acid (IC 50 = 1.318 ± 0.59 µg/mL) and corosolic acid (IC 50 = 1.01 ± 0.06 µg/mL). Our approach allowed the identification of antileishmanial compounds from the crude extracts in only a small number of steps and can be easily adapted for use in the discovery workflows of several other natural products.Molecules 2019, 24, 4536 2 of 13 of the human population to the parasite, and human behavior [3]. The disease is characterized by four forms; visceral leishmaniasis, post-kala-azar dermal leishmaniasis, cutaneous leishmaniasis, and mucocutaneous leishmaniasis [4]. Available medical treatments are not sufficient because of their extensive toxicity (e.g., the nephrotoxicity of amphotericin B, the teratogenicity of miltefosine), their lack of efficacy, their expensive cost (amphotericin B), the development of drug resistance (sodium stibogluconate and meglumine antimoniate), and non-specific targeting [4,5]. Fexinidazole, a 5-nitroimidazole, was recently in phase II of clinical trials on visceral leishmaniasis but exhibited a lack of efficacy, and in 2018 no new lead molecules were included in clinical trials [4].Control of leishmaniasis by traditional approaches (vector control and treatment of patients) is facing the difficulties of identifying new cost-effectively produced bioactive molecules able to be produced on an industrial scale, rapid development of drug resistance, and the lack of access to drugs by the threatened populations. These issues are at the heart of concerns of the WHO regarding the control of tropical diseases [6]. Therefore, combination therapies are used to improve the efficacy of antileishmanial therapies and can shorten treatment duration [7]. Furthering our fundamental knowledge through original approaches, herein concerning the activity of natural compounds from a promising plant species active against Leishmania spp., is vital to ov...

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“…These organs, however, have distinct profiles of specific saponins, which may be explained by the adaptation of each ecotype to its biotic environment. A previous study, for example, showed that saponins derived from hederagenin glycoside in M. truncatula have antifungal activity 38 . Our study confirmed a higher level of these compounds in the strains resistant to A. euteiches (DZA45.5 and A17) than it is in the sensitive strain F83.…”

Section: Resultsmentioning

confidence: 99%

MS-CleanR: A feature-filtering approach to improve annotation rate in untargeted LC-MS based metabolomics

Fraisier-Vannier

Chervin

Cabanac

et al. 2020

Preprint

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5Untargeted metabolomics using liquid chromatography-mass spectrometry (LC-MS) is currently the euteiches; a group of glycosylated triterpenoids overrepresented in resistant lines were identified as 2 9candidate compounds conferring pathogen resistance. MS-CleanR is implemented through a Shiny 3 0 interface for intuitive use by end-users (available at: https://github.com/eMetaboHUB/MS-CleanR). 3 1

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Triterpenoid Glycosides from Medicago sativa as Antifungal Agents against Pyricularia oryzae

Cited by 41 publications

References 38 publications

Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

MS-CleanR: A feature-filtering approach to improve annotation rate in untargeted LC-MS based metabolomics

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