Triterpenes from the surface layer of Poria cocos

Dehydrotrametenonic Acid and Dehydroeburiconic Acid fromPoria cocosand Their Inhibitory Effects on Eukaryotic DNA Polymerase α and β

“…11) The above evidence, coupled with the comparison of 13 C-and 1 H-NMR data ( Table 1) with those of dehydroeburiconic acid [2; (20)-3-oxo-24-methylenelanosta-7,9(11)-dien-24-oic acid] 3,12) and analyses of 1 H-1 H COSY, HMQC, and HMBC spectra, indicated that 1 was a structure of (20)-3-oxolanosta-7,9(11),24-trien-24-oic acid which we named dehydrotrametenonic acid (Fig. 1).…”

“…1) Whereas the inner parts, called Fu-Ling in Chinese, of the sclerotia of P. cocos are reported to have an invigorating activity in addition to diuretic and sedative activities, the epidermis (FuLing-Pi in Chinese) of the sclerotia is reported to have only a diuretic activity and no invigorating activity. 1) Various lanostane-type triterpene acids have been reported from the inner parts 2) and the epidermis 3) of the sclerotia of P. cocos. Several of these acids had potent inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice 4,5) and on Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA, 2) as well as cytotoxicities against some human cancer cells.…”

“…Compound 2 was identified by spectral comparison with the literature data. 3) Compounds 1 and 2 were tested in an enzymatic inhibition assay of eukaryotic pol and pol in vitro. Pol was purified from calf thymus by immunoaffinity column chromatography, as reported previously.…”

Advanced ultra-performance liquid chromatography–photodiode array–quadrupole time-of-flight mass spectrometric methods for simultaneous screening and quantification of triterpenoids in Poria cocos

“…The main chemical constituents of P. cocos are polysaccharides, triterpenoids, egosterol and proteins (Tai, Akahori, & Shingu, 1992;Tai, Shingu, Kikuchi, Tezuka, & Akahori, 1995a;Tai, Shingu, Kikuchi, Tezuka, & Akahori, 1995b;Wang & Wan, 1998;Yang & Bao, 2005), in which triterpenoids are found to possess interesting pharmacological actions such as cytotoxic and anti-oxidant (Zhou et al, 2008), anti-cancer (Gapter, Wang, Glinski, & Ng, 2005), anti-inflammatory (Yasukawa et al, 1998), inhibition of free radical-induced lysis of red blood cells (Sekiya, Goto, Shimada, & Terasawa, 1998) and inhibition of calf DNA polymerase a and rat DNA polymerase b (Akihisa et al, 2004;Kaminaga et al, 1996). Up to now, more than 80 triterpenoid compounds were isolated and identified from P. cocos with the above-mentioned bioactivities.…”

A novel DNA topoisomerase inhibitor: dehydroebriconic acid, one of the lanostane‐type triterpene acids from Poria cocos

“…3,4) Whereas the inner parts, called Fu-Ling in Chinese, of the sclerotia of P. cocos were reported to have an invigorating activity in addition to diuretic and sedative activities, the epidermis (Fu-Ling-Pi in Chinese) of the sclerotia is reported to have only a diuretic activity and no invigorating activity. 3) Both the inner parts [4][5][6][7][8] and the epidermis 9,10) of the sclerotia of P. cocos were reported to contain several lanostane-type triterpene acids, 9,10) which were suggested to be the major medicinal components of the plant. Several of the lanostane-type triterpene acids were revealed to possess potent inhibitory effects on tumor cell growth.…”