Triterpenes from birch bark extract beneficially affect wound healing

Naumann, Katrin; Ebeling, Sandra; Laszczyk, Melanie N.; Scheffler, Armin; Merfort, Irmgard

doi:10.1055/s-0030-1264873

Cited by 2 publications

(1 citation statement)

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“…The results of the BrdU incorporation assay revealed no mitogenic effect of VALE and OA on NIH/3T3 fibroblasts under the experimental conditions used. Enhanced NIH/3T3 cell numbers in an artificial wound by triterpene containing birch bark extract as well as isolated triterpenes in a concentration-dependent manner were also reported by Naumann et al [25]. In their study, fibroblast proliferation as well as cell migration was influenced.…”

Section: Discussionsupporting

confidence: 69%

Effects of Lipophilic Extract ofViscum albumL. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes

Kuonen

Weissenstein

Urech

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Viscum album L. lipophilic extract (VALE) contains pharmacologically active pentacyclic triterpenes that are known to exhibit immunomodulatory, antitumor, and wound healing activity. Preliminary clinical observations indicate that VALE was able to influence cutaneous wound healing in vivo. The objective of this study was to investigate wound closure related properties of VALE in vitro. As measured in a wound healing assay, VALE and its predominant triterpene oleanolic acid (OA) significantly and dose dependently promoted the migration of NIH/3T3 fibroblasts in vitro, thereby leading to an enhanced wound closure. Compared to the negative control, maximal stimulation by 26.1% and 26.2%, respectively, was attained with 10 μg/mL VALE and 1 μg/mL OA. Stimulation of proliferation in NIH/3T3 fibroblasts by VALE and OA could be excluded. At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes. In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed. The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE. These data support the casual observation that Viscum album L. lipophilic extract might modulate wound healing related processes in vivo.

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Section: Discussionsupporting

confidence: 69%

Effects of Lipophilic Extract ofViscum albumL. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes

Kuonen

Weissenstein

Urech

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

Freiesleben

Soelberg

Nyberg

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

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Triterpenes from birch bark extract beneficially affect wound healing

Cited by 2 publications

References 0 publications

Effects of Lipophilic Extract ofViscum albumL. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes

Effects of Lipophilic Extract ofViscum albumL. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes

Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

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