Triterpenes and Meroterpenes with Neuroprotective Effects from <i>Ganoderma leucocontextum</i>

Chen, Hongyu; Zhang, Jinjin; Ren, Jinwei; Wang, Wenzhao; Xiong, Weiping; Zhang, Yaodong; Li, Bao; Liu, Hongwei

doi:10.1002/cbdv.201700567

Cited by 30 publications

(30 citation statements)

References 26 publications

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“…The steady-state activation curve of I h in the presence of GTs was shifted along voltage axis toward the less depolarized potential. The IC 50 value (i.e., 11.7 µg/mL) required for GTs-mediated inhibition of I h observed in GH 3 cells is similar to that used for anti-oxidative or neuroprotective properties [2,3,4,15,16,17,46,47]. Such intriguing actions could be of pharmacological relevance and appear to be upstream of its effects on oxidative stress occurring inside the cell.…”

Section: Resultsmentioning

confidence: 67%

“…Triterpenoids have molecular weights ranging from 400 to 600 kDa, and their chemical structure is complex and noted to be highly oxidized [9]. The triterpenoid fraction of Ganoderma , consisting of more than 300 lanostane-tetracyclic compounds, has been growingly demonstrated to be effective at exerting an array of biological actions such as that known either to provide effective antioxidant activities for prevention of myocardial injury, or to produce neuroprotective actions [2,3,4,5,6,10,11,12,13,14,15,16,17,18,19,20,21]. Ganoderma triterpenoids (GTs) could also suppress inflammatory response by directly scavenging the free radicals or systemically enhancing the antioxidant enzymes, thereby lowering lipid peroxidase in chicken livers or mice [2,3,8,17,20,21,22,23].…”

Section: Introductionmentioning

confidence: 99%

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Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Chang

Gao

et al. 2019

Molecules

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The triterpenoid fraction of Ganoderma (Ganoderma triterpenoids, GTs) has been increasingly demonstrated to provide effective antioxidant, neuroprotective or cardioprotective activities. However, whether GTs is capable of perturbing the transmembrane ionic currents existing in electrically excitable cells is not thoroughly investigated. In this study, an attempt was made to study whether GTs could modify hyperpolarization-activated cation currents (Ih) in pituitary tumor (GH3) cells and in HL-1 atrial cardiomyocytes. In whole-cell current recordings, the addition of GTs produced a dose-dependent reduction in the amplitude of Ih in GH3 cells with an IC50 value of 11.7 µg/mL, in combination with a lengthening in activation time constant of the current. GTs (10 µg/mL) also caused a conceivable shift in the steady-state activation curve of Ih along the voltage axis to a more negative potential by approximately 11 mV. Subsequent addition of neither 8-cyclopentyl-1,3-dipropylxanthine nor 8-(p-sulfophenyl)theophylline, still in the presence of GTs, could attenuate GTs-mediated inhibition of Ih. In current-clamp voltage recordings, GTs diminished the firing frequency of spontaneous action potentials in GH3 cells, and it also decreased the amplitude of sag potential in response to hyperpolarizing current stimuli. In murine HL-1 cardiomyocytes, the GTs addition also suppressed the amplitude of Ih effectively. In DPCPX (1 µM)-treated HL-1 cells, the inhibitory effect of GTs on Ih remained efficacious. Collectively, the inhibition of Ih caused by GTs is independent of its possible binding to adenosine receptors and it might have profound influence in electrical behaviors of different types of electrically excitable cells (e.g., pituitary and heart cells) if similar in vitro or in vivo findings occur.

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Section: Resultsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Chang

Gao

et al. 2019

Molecules

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“…The aromatic meroterpenoids have attracted the attention of many phytochemists, chemists, and pharmocologists, due to their diverse structures and significant bioactivities (antioxidant, anti-HIV protease, and antifibrotic activities) [13,14,15,16]. Meanwhile, some oxygenated lanostane triterpenoids also showed potent free radical scavenging activities and neuroprotective activity [17,18]. These previous studies suggested Ganoderm mushrooms might be a novel resource for natural antioxidant exploration.…”

Section: Introductionmentioning

confidence: 99%

Triterpenes and Aromatic Meroterpenoids with Antioxidant Activity and Neuroprotective Effects from Ganoderma lucidum

et al. 2019

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Reactive oxygen/nitrogen species generated in the human body can cause oxidative damage associated with many degenerative diseases such as atherosclerosis, dementia, coronary heart diseases, aging, and cancer. There is a great interest in developing new antioxidants from Ganoderma fungus due to its low toxicity. As part of our ongoing search for antioxidative constituents from the fruiting bodies of Ganoderma lucidum, the chemical constituents were investigated and seven secondary metabolites, including one new lanostane triterpene (1), two known aromatic meroterpenoids (6–7), and four known triterpenes (2–5), were isolated by a series of chromatographic methods. The structures of the seven compounds were elucidated by spectroscopic techniques. The isolated compounds were tested in vitro for antioxidant potencies and neuroprotective activities against H2O2 and aged Aβ-induced cell death in SH-SY5Y cells. As a result, compounds 1, 6, and 7 exhibited potent antioxidant and neuroprotective activities. Additionally, all isolated compounds were tested for radical scavenging activities. Compounds 6 and 7 showed the comparable free radical scavenging activities with the standard drug in both ABTS (2, 2’-azobis (3-ethylbenzothiazole-6-sulfonaic acid)) and ORAC (oxygen radical absorbance capacity) experiments. The results from this study suggested that G. lucidum and its metabolites (especially the meroterpenoids) may be potential functional food ingredients for the antioxidation and prevention of neurogenerative diseases.

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“…Ganoderma leucocontextum, called "Bai-Rou-Ling-Zhi (白肉灵芝)" with white context, has been regarded as folk medicine in the Southwestern China for the prevention and treatment of various diseases. In the last five years, it has been reported that its bioactive compounds have various effects, including anti-diabetic, antitumor, neuroprotective, pancreatic lipase inhibition [31][32][33]. In this study, we found that ethanol extract and active fraction (GLEA) inhibited cancer growth in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 70%

“…The fruit bodies of Ganoderma leucocontextum have been used in folk medicine in the prevention and treatment of diseases. Bioactive compounds isolated from Ganoderma leucocontextum were mainly lanostane triterpenes, which have several biological activities, including anti-tumor growth, inhibition of HMG-CoA, inhibition of pancreatic lipase, and neuroprotective activities [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Ganoderiol F purified from Ganoderma leucocontextum retards cell cycle progression by inhibiting CDK4/CDK6

Xie

Peng

et al. 2019

Cell Cycle

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This study was designed to purify molecules possess anti-cancer cell activity from the fruit body of Ganoderma leucocontextum. Bio-activity-guided purification and chromatographic separation of Ganoderma leucocontextum extract led to the enrichment of bioactive fractions and isolation of a single compound. The purified compound was identified as Ganoderiol F, which induced cancer cell death. In the in vivo experiments, we founded ethanol extract and ethyl acetate fraction inhibited tumor growth in the mice injected with 4T1 cells. We found that Ganoderiol F-mediated suppression of breast cancer cell viability occurred through cell cycle arrest. Ganoderiol F downregulated expression of cyclin D, CDK4, CDK6, cyclin E and CDK2 and inhibited cell cycle progression arresting the cells in G1 phase. In addition, Ganoderiol F up-regulated proapoptotic Foxo3, down-regulated anti-apoptotic c-Myc, Bcl-2 and Bcl-w leading to apoptosis in human breast cancer cells MDA-MB-231. These results showed that c-Myc, cyclin D-CDK4/CDK6 and cyclin E-CDK2 are the central components of Ganoderiol F regulation of cell cycle progression. Hence Ganoderiol F may serve as a potential CDK4/CDK6 inhibitor for breast cancer therapy.

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Triterpenes and Meroterpenes with Neuroprotective Effects from Ganoderma leucocontextum

Cited by 30 publications

References 26 publications

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Triterpenes and Aromatic Meroterpenoids with Antioxidant Activity and Neuroprotective Effects from Ganoderma lucidum

Ganoderiol F purified from Ganoderma leucocontextum retards cell cycle progression by inhibiting CDK4/CDK6

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