Triterpene glycosides of the leaves ofFatsia japonica

Kemoklidze, Z. S.; Dekanosidze, G. E.; Dzhikiya, O. D.; Vugal'ter, M. M.; Кемертелидзе, Э. П.

doi:10.1007/bf00579450

Cited by 6 publications

(1 citation statement)

References 3 publications

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“…Ultrapure water for HPLC analysis was obtained from a Millipor Classic purification system. Fatsioside D has been isolated from "Fatsiflogin" by previously described method [7]and the purity determined by HPLC was 99,0%. NMR: Structure elucidation was carried out using 1 H NMR (Bruker Avance 400 MHz), 13 C (Bruker Avance 100 MHz).The HPLC system consisted of an Agilent Technologies Model 1100 liquid chromatography, equipped with a vacuum degasser, a quaternary pump, an auto-sampler and a photodiode array detector (DAD) and MS.…”

Section: 2equipment and Reagentsmentioning

confidence: 99%

Development of Analytical Procedure for the Quantification of “Fatsiflogin”

Getia¹,

Mshvildadze²,

Pichette³

et al. 2017

IOSRPHR

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:"Fatsiflogin" is an original, nonsteroidal para-medical preparation from the leaves of Fatsia japonica with anti-rheumatic, anti-inflammatory and analgesic activities. Fatsioside D (tetraosid of hederagenin) as one of the major compound of "Fatsiflogin", was selected as a chemical markerfor the quantitative validation. The objective of this study is to develop a simple and reliable HPLC method for the determination of Fatsiosid D for quality control studies of "Fatsiflogin". FatsiosideD was purified by preparative HPLC and the structure of the compound was determined by NMR. HPLC separation was performed on an intertsil prep-ODS C18 column, with a solvent system water-acetonitrile. The UV detection is performed at 205 nm. The proposed HPLC method is linear in the range studied (r 2 > 0.999) for all the analytes. The method is precise with intra-and inter-day variations of less than 3.34%. The mean recoveries of the analytes range from 99.68 to 100.29%. The method is successfully applied to the quantification of Fatsioside D. The results indicated that developed HPLC method could be used for the quality control of "Fatsiflogin".

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Section: 2equipment and Reagentsmentioning

confidence: 99%