Trisomy 7 in keratoacanthoma and squamous cell carcinoma detected by fluorescence in‐situ hybridization

Abstract

Keratoacanthoma (KA) is generally considered to be a clinically and histologically distinct entity, but it often remains difficult to separate from well-differentiated squamous cell carcinoma (WDSCC). Recently, trisomy 7 has been identified in squamous cell carcinoma of the skin. In this study, we examined classical KA (n = 6), WDSCC (n = 7) and squamous cell carcinoma with KA-like features (SCC-KA) (n = 8) for trisomy 7 by fluorescence in-situ hybridization (FISH) to determine if this chromosomal abnormality is unique to squamous lesions diagnosed as WDSCC, or shared by both KA and SCC. In addition, the pertinent clinical-histopathologic findings were summarized. Trisomy 7 was identified in one KA, one SCC-KA and two WDSCC. This study demonstrates that there is a chromosomal abnormality shared by KA and SCC, providing further evidence that KA is most likely a form of SCC. Further studies are required to determine if trisomy 7 in these lesions is of prognostic significance.

MYCgene numerical aberrations in actinic keratosis and cutaneous squamous cell carcinoma

“…FISH studies have been demonstrated to be feasible in cutaneous tumours, revealing the presence of trisomy 7 in SCC 13 as well as 3p21 genetic deletion in poorly differentated SCCs and SCCs in situ. 14 In view of the numerous studies suggesting a relationship between MYC and SCC, the aim of our study was to investigate whether SCCs and ⁄or their precursor lesions (AKs) harboured MYC genomic aberrations.…”

“…FISH studies have been demonstrated to be feasible in cutaneous tumours, revealing the presence of trisomy 7 in SCC 13 as well as 3p21 genetic deletion in poorly differentated SCCs and SCCs in situ 14 …”

Cytogenetic alterations in nonmelanoma skin cancer: A review

“…Furthermore, Nangia et al (2001) demonstrated a correlation between trisomy 6 and metastatic potential in BCCs. Trisomy 7 has been observed in both SCC and KA, although not recurrently (Cheville et al, 1995). Dö bler et al (1999) investigated a number of common loci (3p21, 17p13, and 9p21) often observed to undergo deletion in SCC, BCC, KA, and SCC in situ.…”

Evaluation of aneuploidy frequency for chromosomes 6 and 17 in eyelid tumours using the FISH technique

“…To solve these shortcomings, interphase fluorescence in situ hybridization (I-FISH), a useful technique for investigating chromosomal alterations in solid tumours, has been applied to interphase nuclei of tumour tissue from different anatomical sites. In both BCC and SCC tumours, previous FISH studies have reported the presence of chromosome alterations (Koyama et al, 2000;Cheville et al, 1995). However, in the literature there has not been any study regarding chromosomal alterations to the eyelid with BCC and SCC tumours.…”