Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistry

Effect of Terminal Labeling of Fluorescent Dyes on Ribozyme Activity

“…This is the evidence of strong interaction between mercury and sulfur since disulfides don't interact with the mercury in the gel matrix with APM. 10 The observed rate constants for the thiophosphorylation of ribozymes with and without fluorescent dyes are shown in Table 1. ).…”

Inactivation of the Ribozyme with Fluorescent Dyes

“…2). [9][10][11] The mobility of RNA that carry thiophosphate monoester is diminished, compared with non-thiophosphated one. This is the evidence of the strong interaction between mercury and sulfur.…”

“…Disulfides don't interact with the mercury in the gel matrix with APM. 10 Therefore, a ribozyme103, a truncated version of Kin.46 was incubated with ATPɣS and the products were separated by PAGE using gels that contained APM. The initial velocities for the thiophosphorylation of ribozymes with and without two fluorescent dyes were plotted as a function of assay time.…”

Requirement of the Composition of RNA-RNA Duplex for the Ribozyme Activity

“…2). [7][8][9] The mobility of RNA that carry thiophosphate monoester is diminished, compared with nonthiophosphated one. This is the evidence of the strong interaction between mercury and sulfur.…”

“…Disulfides don't interact with the mercury in the gel matrix with APM. 8 Therefore Figure 2. Kinetic assay of four-stranded ribozymes.…”