Triply Triggered Doxorubicin Release From Supramolecular Nanocontainers

Abstract

The synthesis of a supramolecular double hydrophilic block copolymer (DHBC) held together by cucurbit[8]uril (CB[8]) ternary complexation and its subsequent self-assembly into micelles is described. This system is responsive to multiple external triggers including temperature, pH and the addition of a competitive guest. The supramolecular block copolymer assembly consists of poly(N-isopropylacrylamide) (PNIPAAm) as a thermoresponsive block and poly(dimethylaminoethylmethacrylate) (PDMAEMA) as a pH-responsive block. Moreover, encapsulation and controlled drug release was demonstrated with this system using the chemotherapeutic drug doxorubicin (DOX). This triple stimuli-responsive DHBC micelle system represents an evolution over conventional double stimuli-responsive covalent diblock copolymer systems and displayed a significant reduction in the viability of HeLa cells upon triggered release of DOX from the supramolecular micellar nanocontainers.

Artificial molecular and nanostructures for advanced nanomachinery

“…[42][43][44][45] For instance, Lee and Scherman et al have demonstrated the release of cancer drugs from hierarchical nanocarriers can be controlled by three orthogonal, biologically relevant triggers (pH, temperature and a specific molecular cue) that can result in disassembly of the micelles, releasing doxorubicin. 46 This controlled release reduces toxicity to other areas of the body. A wide range of stimuli-responsive polymeric structures have been designed, governed by hydrophobic interactions, which can selectively release molecules in response to certain stimuli.…”

Latest Advances in Antibacterial Materials

“…The macrocyclic host, cucurbit [7]uril (CB [7]) was chosen as the model. Cucurbit[n]urils (CB[n]s) are a family of water-soluble macrocyclic hosts, which have a hydrophobic cavity capable of the binding of one or two guest molecules depending on the size of di®erent CB[n]s. [22][23][24] CB [7] possesses good solubility in water and strong a±nity for one guest molecule. [25][26][27][28][29][30][31] The authors employed CB [7] to bind with the positive charge and hydrophobic component on "-poly-l-lysine hydrochlorides to construct the polypseudorotaxane for antibacterial regulation.…”

pH‐ and β‐cyclodextrin‐responsive micelles based on polyaspartamide derivatives as drug carrier

“…The final product was dialyzed against deionized water through dialysis tube (M w cutoff: 8000-14,000 g mol 21 ) for 1 week to remove the unreacted mPEG (see Supporting Information Fig. S5).…”