Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Ibrahim, Yusran; Soelistyowati, Dinar Tri; Carman, Odang

doi:10.19027/jai.16.1.76-82

Cited by 5 publications

(5 citation statements)

References 18 publications

(20 reference statements)

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“…Many oocytes and follicles are degraded or damaged in ovarian triploid tilapia, and very few do not reach mature oocytes (immature oocytes). The development of oocytes is facing obstacles significantly on triploid tilapia, striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) (Ibrahim et al 2017;Carman et al 2022), Atlantic salmon Salmo salar (Linnaeus, 1758) (Murray et al 2018), and yellowtail tetra Astyanax altiparanae (Garutti & Britski, 2000) (do Nascimento et al 2017). Conversely, triploid salmon specifically demonstrate the delay of oocyte growth, and the degree of oocyte development differs.…”

Section: Discussionmentioning

confidence: 99%

Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages

CARMAN,

MUKTI,

ZAIRIN JR.

et al. 2023

Biodiversitas

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Abstract. Carman O, Mukti AT, Zairin JR M, Alimuddin. 2023. Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages. Biodiversitas 24: 4235-4242. Triploidization has been known as a chromosome set manipulation strategy to produce sterile fish. This study aimed to examine the reproductive and sterility performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages. The triploidization process using heat shock of 41°C for four minutes at four Minutes After Fertilization (MAF) of the zygote old made the population of triploid tilapia. Two steps of fish rearing were conducted, namely indoor fish rearing and outdoor fish grow-out. Fish were reared in a controlled laboratory (indoor) at the Reproduction and Genetics Laboratory of Aquatic Organisms, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University for four months, and then fish grow-out was performed in a controlled pond for two months. Reproductive performances such as Hepatosomatic Index (HSI), Gonadosomatic Index (GSI), gonadal development, and sterility of triploid and diploid for both male and female tilapias were analyzed. These results showed that triploid tilapia generally indicated late gonadal development and growth compared to diploids before and during maturation. Triploid female tilapia showed a lower HSI than diploid female tilapia (P<0.05). Otherwise, triploid and diploid males showed the same HSI. GSI of triploid males and females were lower than diploid males and females (P<0.05) at every age, namely three-, four-, five- and six-month-old fish. Based on the size and histology of the gonad, triploid male and female tilapias showed sterile characteristics with gametogenesis inhibition.

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Section: Discussionmentioning

confidence: 99%

Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages

CARMAN,

MUKTI,

ZAIRIN JR.

et al. 2023

Biodiversitas

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“…Studi triploidisasi mengunakan suhu teknik kejut telah dilakukan pada beberapa ikan spesies seperti ikan Selais (Alawi et al 2009), nila tilapia (Mukti et al 2009), ikan mas (Mukti et al 2001), lele afrika (Nurasni 2012) dan ikan patin (Ibrahim et al 2017). Studi sebelumnya menunjukkan pertumbuhan kinerja ikan triploid lebih cepat daripada ikan diploid karena teknik triploidisasi sangat menjanjikan untuk meningkatkan kinerja pertumbuhan ikan serukan.…”

Section: Pendahuluanunclassified

“…Pada penelitian Alawi et al (2009) pemberian kejutan panas dengan suhu kejutan 40 C dan lama kejutan 1-5 menit dapat menginduksi triploid ikan selais (Cryptopterus lympok) dengan tingkat keberhasilan triploid mencapai 41,7-91,7%. Menurut Ibrahim et al (2017) berpendapat bahwa pemberian kejutan panas pada suhu 42 C dan lama kejutan 2 menit mampu mendapatkan ikan patin siam (Pangasianodon hypopthlalmus) dengan gejala sterilitas pada ikan patin siam. Susilo et al (2018) menambahkan rekayasa set kromosom dengan kejut panas pada suhu 40 C dan lama kejutan 1,5-2 menit mampu menghasilkan keberhasilan ikan triploid mencapai 43-53%.…”

Section: Persentase Ikan Triploidunclassified

OPTIMASI WAKTU PEMBELAHAN SEL DAN SUHU KEJUTAN TERHADAP TINGKAT KEBERHASILAN TRIPLOID IKAN SERUKAN (Osteochilus sp.)

Zaleha¹,

Ibrahim

Saputra

2021

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This study aims to determine the optimization of cell division and temperature shock on the triploid success rate of the seurukan fish (Osteochilus sp.) By allowing different temperature shock on the degree of fertilization, the degree of hatching, the success rate of the triploid, the daily growth rate and the survival rate of the serukan fish's (Osteochilus sp.). In this research, the age of the zygote used was 20 minutes old after fertilization, the shock duration was 2 minutes and the temperature shock were 40, 41, and 42°C. larvae were looked after for 14 days and fed with egg yolk and Artemia sp. using Adlibitum with a frequency of 2 times a day. The container used in this study was a 12-unit jar with a volume of 2.5 liters of water equipped with aeration with 15 larvae for each replication. The results showed that larvae that treated in different heat shocks had a significant effect (P <0.05) on the fertilization degree 49.3 ± 6.9% with a temperature shock of 41°C, as for hatching degrees 49.2 ± 11.1% with a temperature shock of 41°C, and survival rate is 84.4 ± 7.6% at temperature shock of 41°C, while daily growth rate had no significant effect (P> 0.05) 5.15 ± 1.0% at temperature shock of 41°C, further 60% of triploid success rate with a temperature shock of 42°C.

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“…Telur yang digunakan berjumlah 5000 butir setiap kelompok. Hal yang sama ditemukan Ibrahim et al (2017) pada patin siam triploid memiliki jumlah kromosom 1,5 kali lipat lebih banyak dibandingkan dengan patin siam diploid. Walaupun penghitungan kromosom merupakan metode yang unggul dan tepat dalam penentuan tingkat ploidi, tetapi analisis kromosom membutuhkan keahlian yang tinggi dan waktu yang relatif lama (Caterina et al 2014).…”

Section: Produksi Ikan Diploid Dan Tetraploidunclassified

Ploidy level determination in genetically modified polyploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 based on the number of nucleoli per cell

et al. 2019

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The variation in the maximum number of nucleoli per cell in diploid and tetraploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 in attempts to verify the validity of nucleoli counting as an indirect method for polyploidy identification in fish. The aims of this research is to determine ploidy level of striped catfish based on maximum number of nucleoli per cell. Diploid striped catfish was produced by fertilizing eggs without thermal-shock and tetraploid fish was obtained by fertilizing eggs and applying thermal-shock induction with 4oC for 25 minutes at zygotic age 28 minutes after fertilization prior to first cleavage stage of zygote. The hatching rate of diploid group and tetraploid group were 81,35±0,73% and 3,39±1,78% and survival rate during 15 days rearing were 88,67±5,25% and 83,33±5,73%, respectively. The frequency of one, two, three, and four nucleoli per cell were counted based on each sample observation of 450-550 cells. Cells of diploid individuals had one, and maximum two nucleoli per cell, while tetraploid there were one, two, three, and maximum four nucleoli per cell. Anomaly in the silver-stained appearance at the maximum number of three nucleoli per cell indicated tetraploid individuals as verified by chromosome counting method. Ploidy level determination of striped catfish using the number of nucleoli per cell has a potential for rapid identification.AbstrakPenentuan variasi jumlah maksimum nukleolus per sel pada individu diploid dan tetraploid patin siam Pangasianodon hypophthalmus Sauvage, 1878 dilakukan dalam upaya verifikasi ketepatan penghitungan nukleolus sebagai metode tidak langsung dalam identifikasi ikan poliploid. Penelitian ini bertujuan menentukan tingkat ploidi ikan patin siam berdasarkan jumlah maksimum nukleolus per sel. Diploid patin siam dihasilkan melalui fertilisasi buatan antara telur dan sperma tanpa kejutan suhu sedangkan tetraploid patin siam dihasilkan melalui fertilisasi antara telur dan sperma serta di induksi menggunakan kejutan suhu 4oC dengan durasi kejutan 25 menit pada umur zigot 28 menit setelah fertilisasi sesaat sebelum mitosis I. Persentase penetasan kelompok perlakuan diploid 81,35±0,73% dan kelompok perlakuan tetraploid 3,39±1,78%. Sintasan kelompok perlakuan diploid 88,67±5,25% dan kelompok tetraploid 83,33±5,73% selama 15 hari pemeliharaan. Frekuensi satu, dua, tiga dan empat nukleoli per sel dihitung berdasarkan pengamatan 450-550 sel setiap individu yang diamati. Sel individu diploid memiliki satu dan maksimum dua nukleoli per sel, sedangkan individu tetraploid memiliki satu, dua, tiga, dan maksimum empat nukleoli per sel. Anomali muncul melalui pewarnaan perak nitrat pada jumlah maksimum tiga nukleoli per sel menunjukkan individu tersebut tetraploid yang diverifikasi menggunakan metode penghitungan kromosom. Penentuan ploidi pada ikan patin siam menggunakan penghitungan jumlah nukleolus per sel memiliki potensi dalam identifikasi secara cepat.

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Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Cited by 5 publications

References 18 publications

Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages

Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages

OPTIMASI WAKTU PEMBELAHAN SEL DAN SUHU KEJUTAN TERHADAP TINGKAT KEBERHASILAN TRIPLOID IKAN SERUKAN (Osteochilus sp.)

Ploidy level determination in genetically modified polyploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 based on the number of nucleoli per cell

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