Triploid and Aneuploid Hybrids from Diploid-diploid Intergeneric Crosses between Citrus Cultivar ‘Kiyomi’ Tangor and Meiwa Kumquat (Fortunella crassifolia Swingle) for Seedless Breeding of Kumquats

Abstract

In order to produce new seedless kumquat cultivars, we carried out an intergeneric cross between 'Kiyomi' tangor [Citrus unshiu Marcow. × C. sinensis (L.) Osbeck] and Meiwa kumquat (Fortunella crassifolia Swingle), obtaining 2 normal seeds and 7 undeveloped seeds. These seeds were cultivated on Murashige and Tucker medium, and the 2 normal seeds germinated and developed. The results of genome size analysis by flow cytometry revealed that both seedlings were triploids and that the difference in genome size corresponded to more than one chromosome in the 2 seedlings. Chromosome observation confirmed diploid (2n = 2x = 18) in both parents, aneuploid with 28 chromosomes (2n = 28) for one of the seedlings, and triploid (2n = 3x = 27) for the other seedling. Random amplified polymorphic DNA (RAPD) and cleaved amplified polymorphic sequence (CAPS) analyses proved that the seedlings were intergeneric hybrids between 'Kiyomi' tangor and Meiwa kumquat, with the maternal organelle genome. These hybrids have the potential to be released as a cultivar after further tree and fruit evaluations, and for use as cross-parents in seedless kumquat breeding.

Identification and Characterization of a Loquat Aneuploid with Novel Leaf Phenotypes

“…Aneuploid occurs naturally in some plants such as Phalaenopsis (Zhuang et al, 2007), and is frequently found in interploid hybridization, especially in crosses involving a triploid (Ezura et al, 1994;Henry et al, 2009;Vogt and Rowe, 2011). Aneuploid has also been reported in some fruit trees, such as guava (Mohammed, 1974), apple (Chagn e et al, 2015;Zhang and Sungmin, 2009), pear (Simard et al, 2011), citrus (Yasuda et al, 2010), grapevine (Park et al, 1999), and a few others (Badenes and Byrne, 2012).…”

Identification of Parental Genome Construction and Inherited Morphological Characteristics in Triploid and AneuploidIntergeneric Hybrids from a Diploid−Diploid Cross between Citrus and Fortunella

“…× C. sinensis (L.) Osbeck) and six Fortunella species. On the basis of genome size and DNA analyses, we confirmed that the seedlings were aneuploid (2n = 28) and eutriploid (2n = 3x = 27) intergeneric hybrids [2].…”

Unreduced 2n Pollen Production in ‘Nishiuchi Konatsu’ Hyuganatsu as Inferred by Pollen Characteristics and Progeny Ploidy Level

“…Leaf samples were then chopped with a razor blade in 2 mL chopping buffer containing 1.0% (v/v) Triton X-100, 140 mM mercaptoethanol, 50 mM Na 2 SO 3 , and 50 mM Tris-HCl at pH 7.5, and incubated at room temperature for 5 min according to the preparation method of Yahata et al (2010) and Yasuda et al (2010). Crude samples were filtrated through a Miracloth (Calbiochem, La Jolla, USA) and stained with 25 μg·L −1 propidium iodide (PI).…”