2014
DOI: 10.1039/c3cc45917a
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Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

Abstract: Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.

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Cited by 10 publications
(10 citation statements)
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“…DNA triplexes have evoked potential technological applications which include creating high quality DNA vectors for human gene therapy [ 28 ], nanomachines for monitoring intracellular pH gradient [ 29 ], molecular switches [ 30 , 31 , 32 ], and for developing drug delivery systems [ 33 , 34 ]. They have also been used as biosensors for the recognition and analysis of toxic metal ions [ 35 ], SNPs [ 36 ], DNA methylation [ 37 ] and cancer cells [ 38 ]. Quite recently, DNA triplex formation process has been exploited in developing PCR based biosensor for detection of pathogens [ 39 ], high-throughput assays for measuring activity of DNA topoisomerases and other enzymes involved in DNA topology modification [ 40 ], controlled assembly of liposomes [ 41 ] and as modular probes for DNA detection [ 42 ].…”
Section: Introductionmentioning
confidence: 99%
“…DNA triplexes have evoked potential technological applications which include creating high quality DNA vectors for human gene therapy [ 28 ], nanomachines for monitoring intracellular pH gradient [ 29 ], molecular switches [ 30 , 31 , 32 ], and for developing drug delivery systems [ 33 , 34 ]. They have also been used as biosensors for the recognition and analysis of toxic metal ions [ 35 ], SNPs [ 36 ], DNA methylation [ 37 ] and cancer cells [ 38 ]. Quite recently, DNA triplex formation process has been exploited in developing PCR based biosensor for detection of pathogens [ 39 ], high-throughput assays for measuring activity of DNA topoisomerases and other enzymes involved in DNA topology modification [ 40 ], controlled assembly of liposomes [ 41 ] and as modular probes for DNA detection [ 42 ].…”
Section: Introductionmentioning
confidence: 99%
“…One group is consisting of proteins that act as binders and that are typically used in affinity enrichment assays. These can bind DNA either without or with constrained sequence selectivity (i.e., in the context of short and fixed recognition sequences such as palindromes), or they bind DNA with programmable sequence selectivity (a related potential may be offered by triplex-forming oligonucleotides) . The second group consists of proteins that act as processive readers and recognize nucleobases in a continuous manner as DNA is passing through the protein.…”
Section: New Challenges For Deciphering Epigenetic Cytosine Modificat...mentioning
confidence: 99%
“…Characterising the in vivo effects of DNA methylation on G4 structures may provide a possible therapeutic avenue to further enhance specificity in the tumour environment. Additional DNA modifications, such as 8-oxoguanine [ 142 ] or methylation at CpA dinucleotides [ 143 ] can affect the structural kinetics of non B-DNA formations and have been linked to the silencing of cancer genes in lymphoma and myeloma cell lines [ 144 ]. However, the effect that these could have on non B-DNA structural formation has not attracted substantial scientific investigation.…”
Section: Introductionmentioning
confidence: 99%