Triplex-forming DNA Probe Approach for Silver Detection and the Effect of C-G·C Triplet Distribution on Triplex Stability

Abstract

B esides the knowledge that DNA is the primary hereditary molecule with possessing genetic code hidden in its double stranded helix form (B-form), an increasing number of evidence demonstrate that DNA, due to its non-canonical (non-B-form) structures, have regulatory functions on various cellular processes as well [1]. While duplex form of DNA is built up via Watson-Crick base pairing edges of nucleotide residues, some forms of the DNA are created by binding of auxiliary strands to a duplex DNA via Hoogsten base pairing edges of nucleotides [2,3]. In particular, triplex DNA forms have become one of the primary research areas due to the possibility of targeting regulatory regions of several oncogenes [2][3][4] and that their providing ease to manipulation due to exhibiting high stability in comparison to duplex forms [5].These triplex DNA forms could be created via binding of an auxiliary strand which is either rich in purine or pyrimidine, purine rich strand of the duplex DNA [6].