“…To investigate the effect of amino acid substitutions on both yield and glycan binding phenotype, we employed a grouping method for amino acids 54 . Each amino acid was assigned to one of three groups based on its biophysical properties: nonpolar (V, L, I, M, C, F, W, and Y), small polar (G, A, and P), and polar/charged (S, T, N, Q, H, D, E, K, and R).…”