Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing

Controlling the assembly of DNA in order on a suitable electrode surface is of great significance for biosensors and disease diagnosis, but it is full of challenges. In this work, we creatively assembled DNA on the surface of octadecylamine (ODA)-modified topological insulator (Tls) Bi 2 Se 3 and developed an electrochemical biosensor to detect biomarker DNA of coronavirus disease 2019 (COVID-19). A high-quality Bi 2 Se 3 sheet was obtained from a single crystal synthesized in our lab. A uniform ODA layer was coated in argon by chemical vapor deposition (CVD). We observed and analyzed the assembly and mechanism of single-strand DNA (ssDNA) and double-strand DNA (dsDNA) on the Bi 2 Se 3 surface through atomic force microscopy (AFM) and molecular dynamics (MD) simulations. The electrochemical signal revealed that the biosensor based on the DNA/ODA/Bi 2 Se 3 electrode has a wide linear detection range from 1.0 × 10 −12 to 1.0 × 10 −8 M, with the limit of detection as low as 5 × 10 −13 M. Bi 2 Se 3 has robust surface states and improves the electrochemical signal-to-noise ratio, while the uniform ODA layer guides high-density ordered DNA, enhancing the sensitivity of the biosensor. Our work demonstrates that the ordered DNA/ODA/Bi 2 Se 3 electrode surface has great application potential in the field of biosensing and disease diagnosis.

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“…LOD was obtained at 0.5 pM based on 3σ/m. 44 Compared with the reported electrochemical biosensors, 62,63…”

Section: Simulation Of Bindingmentioning

confidence: 99%

Ordered Assembly of DNA on Topological Insulator Bi₂Se₃ and Octadecylamine for a Sensitive Biosensor

et al. 2023

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“…DNA nanotechnology has shown significant advantages in the construction of SARS-CoV-2 nucleic acid sensors. Recently, Wang et al developed a tetrahedral DNA-functionalized field-effect transistor (FET) sensor for highly sensitive detection of SARS-CoV-2 [137]. Tetrahedral DNA nanostructures were constructed by one-step self-assembly, consisting of a rigid tetrahedral base and a flexible arm.…”

Section: Biosensingmentioning

confidence: 99%

Preparation, applications, and challenges of functional DNA nanomaterials

Zhang

Chu

et al. 2022

Nano Res.

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As a carrier of genetic information, DNA is a versatile module for fabricating nanostructures and nanodevices. Functional molecules could be integrated into DNA by precise base complementary pairing, greatly expanding the functions of DNA nanomaterials. These functions endow DNA nanomaterials with great potential in the application of biomedical field. In recent years, functional DNA nanomaterials have been rapidly investigated and perfected. There have been reviews that classified DNA nanomaterials from the perspective of functions, while this review primarily focuses on the preparation methods of functional DNA nanomaterials. This review comprehensively introduces the preparation methods of DNA nanomaterials with functions such as molecular recognition, nanozyme catalysis, drug delivery, and biomedical material templates. Then, the latest application progress of functional DNA nanomaterials is systematically reviewed. Finally, current challenges and future prospects for functional DNA nanomaterials are discussed.

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“…(g) A pooled, rapid testing ( s) framework for accurate, population-level screening of COVID-19 with high testing efficiency, using a triple-probe tetrahedral DNA framework on a g-FET. Reprinted (adapted) with permission from Wu et al [ 32 ] ©2022 American Chemical Society. …”

Section: Nanomaterials For Rapid Diagnostics: Point-of-care Testing I...mentioning

confidence: 99%

“…2 (b), shows outstanding accuracy in distinguishing COVID-19 from healthy samples when compared with the benchmark RT-PCR assay; which makes this a very promising candidate for a rapid, highly accurate test for health screening in point-of-care settings. In another example, Wu et al have developed [ 32 ] a graphene field-effect transistor (g-FET), functionalized with a triple-probe tetrahedral DNA framework (TDF), for 10-in-1 pooled testing of SARS-CoV-2 RNA as illustrated in Figure 2 (d). Upon testing on clinical samples of nasopharyngeal swabs, the researchers were able to detect as few as 0.025–0.05 copies of viral RNA/ l, which is lower than the detection limit of RT-PCR, with a very fast readout in –4 min., and a high sensitivity of 0.93 and perfect specificity.…”

Section: Nanomaterials For Rapid Diagnostics: Point-of-care Testing I...mentioning

confidence: 99%

Nanomaterials in diagnostics, imaging and delivery: Applications from COVID-19 to cancer

Bardhan

2022

MRS Communications

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In the past two decades, the emergence of nanomaterials for biomedical applications has shown tremendous promise for changing the paradigm of all aspects of disease management. Nanomaterials are particularly attractive for being a modularly tunable system; with the ability to add functionality for early diagnostics, drug delivery, therapy, treatment and monitoring of patient response. In this review, a survey of the landscape of different classes of nanomaterials being developed for applications in diagnostics and imaging, as well as for the delivery of prophylactic vaccines and therapeutics such as small molecules and biologic drugs is undertaken; with a particular focus on COVID-19 diagnostics and vaccination. Work involving bio-templated nanomaterials for high-resolution imaging applications for early cancer detection, as well as for optimal cancer treatment efficacy, is discussed. The main challenges which need to be overcome from the standpoint of effective delivery and mitigating toxicity concerns are investigated. Subsequently, a section is included with resources for researchers and practitioners in nanomedicine, to help tailor their designs and formulations from a clinical perspective. Finally, three key areas for researchers to focus on are highlighted; to accelerate the development and clinical translation of these nanomaterials, thereby unleashing the true potential of nanomedicine in healthcare. Graphical abstract

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Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing

Cited by 35 publications

References 54 publications

Ordered Assembly of DNA on Topological Insulator Bi₂Se₃ and Octadecylamine for a Sensitive Biosensor

Ordered Assembly of DNA on Topological Insulator Bi₂Se₃ and Octadecylamine for a Sensitive Biosensor

Preparation, applications, and challenges of functional DNA nanomaterials

Nanomaterials in diagnostics, imaging and delivery: Applications from COVID-19 to cancer

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Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing

Cited by 35 publications

References 54 publications

Ordered Assembly of DNA on Topological Insulator Bi2Se3 and Octadecylamine for a Sensitive Biosensor

Ordered Assembly of DNA on Topological Insulator Bi2Se3 and Octadecylamine for a Sensitive Biosensor

Preparation, applications, and challenges of functional DNA nanomaterials

Nanomaterials in diagnostics, imaging and delivery: Applications from COVID-19 to cancer

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Product

Resources

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Ordered Assembly of DNA on Topological Insulator Bi₂Se₃ and Octadecylamine for a Sensitive Biosensor

Ordered Assembly of DNA on Topological Insulator Bi₂Se₃ and Octadecylamine for a Sensitive Biosensor