Triple-helix polysaccharides: Formation mechanisms and analytical methods

Guo, Xiao-Yu; Kang, Ji; Xu, Zhengguo; Guo, Qingbin; Zhang, Lifeng; Ning, Haifeng; Cui, Steve W.

doi:10.1016/j.carbpol.2021.117962

Cited by 122 publications

(63 citation statements)

References 93 publications

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“…Statistics has shown that triple helix conformation was the most active spatial conformation of polysaccharides. Therefore, in this way, the excellent biological activity of IOPS might contribute to the triple helix conformation [ 77 , 82 ].…”

Section: Physiochemical and Structural Featuresmentioning

confidence: 99%

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Jia

Xue

et al. 2021

Polymers

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Inonotus obliquus (Chaga mushroom) is a kind of medicine and health food widely used by folk in China, Russia, Korea, and some occidental countries. Among the extracts from Inonotus obliquus, Inonotus obliquus polysaccharide (IOPS) is supposed to be one of the major bioactive components in Inonotus obliquus, which possesses antitumor, antioxidant, anti-virus, hypoglycemic, and hypolipidemic activities. In this review, the current advancements on extraction, purification, structural characteristics, and biological activities of IOPS were summarized. This review can provide significant insight into the IOPS bioactivities as their in vitro and in vivo data were summarized, and some possible mechanisms were listed. Furthermore, applications of IOPS were reviewed and discussed; IOPS might be a potential candidate for the treatment of cancers and type 2 diabetes. Besides, new perspectives for the future work of IOPS were also proposed.

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Section: Physiochemical and Structural Featuresmentioning

confidence: 99%

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Jia

Xue

et al. 2021

Polymers

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“…The bioactivities of biopolymers are affected by their conformations, and polysaccharides that contain triple‐helix structures or not can be determined by Congo red assay (Xu et al ., 2018). The polysaccharides with helical conformation can form complexes with Congo red to make the maximum absorption bathochromic shift under alkaline conditions (Guo et al ., 2021). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Effects of origin and harvest period on characterisation, structure and antioxidant activity of polysaccharides derived from Porphyra haitanensis

Pan

Haichao

et al. 2021

Int J of Food Sci Tech

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In the present study, the influences of different origins and harvest periods on the characterisation, structure and antioxidant activity of Porphyra haitanensis polysaccharides (PHPs) were investigated. PHPR03 obtained from the third harvest period (late December) in Raoping possessed the highest content of sulphate group (14.70%), the lowest viscosity (434.34 mLÁg −1 ) and exhibited better antioxidant activity. FTIR spectrum confirmed that PHPs obtained in different origins and harvest periods had similar functional groups (O-H, C-H and C-O group). Thermal gravimetric analysis suggested that PHPR03 has good thermal stability until 182 °C. PHPR03 microstructure was detected using scanning electron microscopy, and it was mainly irregular flocs. Atomic force microscope data showed that its diameter and height ranged from 1.32 to 1.99 μm and 14.16 to 34.92 nm, respectively. X-ray diffraction and Congo red experiments indicated that PHPR03 belongs to amorphous material with weak rigidity and exists in the form of random coils in an aqueous solution. PHPR03 resulted in a decrease of thiobarbituric acidreactive substances value of ground pork. This study provides the theoretical basis for rational utilisation and further applications in the food industry of PHPs.

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“…The sample of 0.5 mg/mL BCP and 50 mol/L Congo red was prepared in advance. The two reagents mixed with 1 mol/L NaOH solution to adjust the concentration of NaOH [ 37 ]. After mixing, the two sets of NaOH solutions with concentrations of 0, 0.1, 0.2, 0.3, 0.4, and 0.5 mol/L were obtained [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

et al. 2022

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This study explored the preliminary structural characteristics and in vivo anti-tumor activity of an acidic water-soluble polysaccharide (BCP) separated purified from Bupleurum chinense DC root. The preliminary structural characterization of BCP was established using UV, HPGPC, FT-IR, IC, NMR, SEM, and Congo red. The results showed BCP as an acidic polysaccharide with an average molecular weight of 2.01 × 103 kDa. Furthermore, we showed that BCP consists of rhamnose, arabinose, galactose, glucose, and galacturonic acid (with a molar ratio of 0.063:0.788:0.841:1:0.196) in both α- and β-type configurations. Using the H22 tumor-bearing mouse model, we assessed the anti-tumor activity of BCP in vivo. The results revealed the inhibitory effects of BCP on H22 tumor growth and the protective actions against tissue damage of thymus and spleen in mice. In addition, the JC-1 FITC-AnnexinV/PI staining and cell cycle analysis have collectively shown that BCP is sufficient to induce apoptosis and of H22 hepatocarcinoma cells in a dose-dependent manner. The inhibitory effect of BCP on tumor growth was likely attributable to the S phase arrest. Overall, our study presented significant anti-liver cancer profiles of BCP and its promising therapeutic potential as a safe and effective anti-tumor natural agent.

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Triple-helix polysaccharides: Formation mechanisms and analytical methods

Cited by 122 publications

References 93 publications

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Effects of origin and harvest period on characterisation, structure and antioxidant activity of polysaccharides derived from Porphyra haitanensis

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

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