Triple-amiRNA VEGFRs inhibition in pancreatic cancer improves the efficacy of chemotherapy through EMT regulation

Huang, Jianfei; Mei, Haijun; Tang, Zhiyuan; Li, Jieying; Zhang, Xiaojing; Lu, Yixiang; Huang, Fang; Jin, Qin; Wang, Zhiwei

doi:10.1016/j.jconrel.2016.11.024

Cited by 31 publications

(21 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VEGF, as a highly specific vascular endothelial growth factor, promotes vascular permeability, vascular endothelial cell migration, proliferation and angiogenesis . To find out the possible mechanism of H 2 S to improved blood supply during skin wound healing, dynamic VEGF expression of the skin wound was measured.…”

Section: Resultsmentioning

confidence: 99%

Exogenous hydrogen sulphide supplement accelerates skin wound healing via oxidative stress inhibition and vascular endothelial growth factor enhancement

Hua

et al. 2019

Experimental Dermatology

View full text Add to dashboard Cite

Hydrogen sulphide (H2S) is an important gasotransmitter with several physiological functions. However, the roles and the detailed mechanisms of H2S on skin wound healing are not known well. In the present study, 129S1/SvImJ mice were intraperitoneally injected with NaHS (50 μmol/kg/d) for 2 weeks. Then, a round wound of 6 mm diameter with depth into the dermis was made. The skin wound area, blood perfusion, superoxide production, malondialdehyde (MDA) levels, total antioxidant capacity (T‐AOC), expression of vascular endothelial growth factor (VEGF), dynamin‐related protein 1 (DRP1) and optic atrophy 1 (OPA1) were measured. After NaHS (50 μmol/L) pre‐administration for 4 hours, cell migration rate, DRP1, OPA1 and α–smooth muscle actin (α‐SMA) expression, superoxide production and mitochondrial membrane potential in primary skin fibroblasts were measured. Tube formation in human umbilical vein endothelial cells (HUVECs) and cell migration in human keratinocytes were also measured. The results showed that NaHS pretreatment significantly accelerated wound healing and improved blood flow in the wound after operation. NaHS increased VEGF expression in the wound and promoted tube formation in HUVECs. Meanwhile, NaHS attenuated reactive oxygen species (ROS) production, suppressed MDA level but restored T‐AOC in the wound. NaHS also promoted skin fibroblasts migration and α‐SMA expression after scratch. Moreover, NaHS alleviated ROS, increased mitochondrial membrane potential, decreased DRP1 but enhanced OPA1 expression in skin fibroblasts after scratch. NaHS also accelerated human keratinocytes migration after scratch. Taken together, exogenous H2S supplementary accelerated the skin wound healing, which might be related to oxidative stress inhibition and VEGF enhancement.

show abstract

Section: Resultsmentioning

confidence: 99%

Exogenous hydrogen sulphide supplement accelerates skin wound healing via oxidative stress inhibition and vascular endothelial growth factor enhancement

Hua

et al. 2019

Experimental Dermatology

View full text Add to dashboard Cite

show abstract

“…Western blotting was performed according to a protocol used in other reports (Zhai et al ., ; Qin et al ., ; Liu et al ., , ,b; Han et al ., ; Huang et al ., ; Zhang et al ., ; Wang et al ., , ). Primary antibodies against PPARα (ab8934, 1:500; Abcam, Cambridge, UK), BDNF (sc‐20981, 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (2118S, 1:2000; Cell Signaling, Danvers, MA, USA) were used.…”

Section: Methodsmentioning

confidence: 99%

Hippocampal PPARα is a novel therapeutic target for depression and mediates the antidepressant actions of fluoxetine in mice

Song

Wang

et al. 2018

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Accordingly, EMT might be neutralized using miR-875-5p, which mediates EGFR downregulation in PCa cells, promoting a radiosensitizing effect (El Bezawy et al, 2017). Additionally, the inhibition of VEGFR (VEGF receptor) expression using three artificial miRNA in PCa cells and xenograft mouse models showed a synergistic effect with standard chemotherapy and was associated with EMT inhibition (Huang et al, 2017). Indeed, it has been recently described that the combination of vaccines with miR-200 agomiRs or shZEB1 significantly inhibited EMT features in melanoma cells and prompted an immune response that blocked melanoma growth and metastasis in mouse models .…”

Section: Are Anti-emt Therapies Feasible?mentioning

confidence: 99%

EMT: Present and future in clinical oncology

et al. 2017

View full text Add to dashboard Cite

Epithelial/mesenchymal transition (EMT) has emerged as a key regulator of metastasis by facilitating tumor cell invasion and dissemination to distant organs. Recent evidences support that the reverse mesenchymal/epithelial transition (MET) is required for metastatic outgrowth; moreover, the existence of hybrid epithelial/mesenchymal (E/M) phenotypes is increasingly being reported in different tumor contexts. The accumulated data strongly support that plasticity between epithelial and mesenchymal states underlies the dissemination and metastatic potential of carcinoma cells. However, the translation into the clinics of EMT and epithelial plasticity processes presents enormous challenges and still remains a controversial issue. In this review, we will evaluate current evidences for translational applicability of EMT and depict an overview of the most recent EMT in vivo models, EMT marker analyses in human samples as well as potential EMT therapeutic approaches and ongoing clinical trials. We foresee that standardized analyses of EMT markers in solid and liquid tumor biopsies in addition to innovative tools targeting the E/M states will become promising strategies for future translation to the clinical setting.

show abstract

Triple-amiRNA VEGFRs inhibition in pancreatic cancer improves the efficacy of chemotherapy through EMT regulation

Cited by 31 publications

References 37 publications

Exogenous hydrogen sulphide supplement accelerates skin wound healing via oxidative stress inhibition and vascular endothelial growth factor enhancement

Exogenous hydrogen sulphide supplement accelerates skin wound healing via oxidative stress inhibition and vascular endothelial growth factor enhancement

Hippocampal PPARα is a novel therapeutic target for depression and mediates the antidepressant actions of fluoxetine in mice

EMT: Present and future in clinical oncology

Contact Info

Product

Resources

About