Triphenyltin biosorption, dephenylation pathway and cellular responses during triphenyltin biodegradation by Bacillus thuringiensis and tea saponin

Jie, Huang; Ye, Jinshao; Ma, Jiawen; Gao, Jiong; Chen, Siqin; Xiao-ling, WU

doi:10.1016/j.cej.2014.03.110

Cited by 27 publications

(9 citation statements)

References 31 publications

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“…B. thuringiensis poza właściwościami owadobójczymi wykazuje zdolność do rozkładu niektórych toksycznych związków organicznych. Z przeglądu literatury wynika, że był on stosowany do rozkładu ftalanu dimetylu [16], fipronilu [57], trifenylocyny [39], bisfenolu A [54] czy policyklicznych węglowodorów aromatycznych (PHA), takich jak np. fenantren, a także niektórych pestycydów np.…”

Section: Nowe Możliwości Wykorzystania Bakterii Bacillus Thuringiensisunclassified

Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny

Gęsicka

Henschke

Barańska

et al. 2020

Postępy Mikrobiologii - Advancements of Microbiology

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Wpłynęło w lutym, zaakceptowano w październiku 2020 r.Streszczenie: Owadobójcze właściwości Bacillus thuringiensis (Bt) sprawiają, że jest to cenny gatunek bakterii dla rozwoju rolnictwa. Produkowane przez Bt białka Cry i Cyt działają w sposób selektywny, dlatego ich stosowanie prowadzi do wyeliminowania tylko larw owadów docelowych. Znane są również inne substancje produkowane przez bakterie Bt, które mogą się przyczynić do eliminacji agrofagów i promowania wzrostu roślin. Ponadto podejmowane są próby stosowania szczepów B. thuringiensis w procesie bioremediacji terenów skażonych toksycznymi związkami organicznymi oraz w medycynie, w zwalczaniu patogenów ludzkich i zwierzęcych oraz komórek nowotworowych.1. Wprowadzenie. 2. Charakterystyka gatunku Bacillus thuringiensis. 3. Czynniki wirulencji Bacillus thuringiensis. 4. Wykorzystanie Bacil lus thuringiensis w nowoczesnym rolnictwie 5. Nowe możliwości wykorzystania bakterii Bacillus thuringiensis. 6. Podsumowanie

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Section: Nowe Możliwości Wykorzystania Bakterii Bacillus Thuringiensisunclassified

Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny

Gęsicka

Henschke

Barańska

et al. 2020

Postępy Mikrobiologii - Advancements of Microbiology

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“…Bacillus thuringiensis was isolated from organotin-contaminated sediment samples collected from an e-waste processing and recycling town called Guiyu in Guangdong Province, China [8]. TPT, DPT, MPT, and Chromatographic methanol were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Strain and Chemicalsmentioning

confidence: 99%

“…Multiple strains with high degradation performance, including Stenotrophomonas maltophilia [6], Brevibacillus brevis [7], and Bacillus thuringiensis, have been screened for their ability to degrade organotins. Several studies have revealed that the mechanisms of TPT biodegradation result in diphenyltin (DPT), monophenyltin (MPT), and tin as degradation products [8]. Endoenzymes, an appropriate amount of exogenous nutrients, and surfactants can accelerate TPT degradation [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have revealed that the mechanisms of TPT biodegradation result in diphenyltin (DPT), monophenyltin (MPT), and tin as degradation products [8]. Endoenzymes, an appropriate amount of exogenous nutrients, and surfactants can accelerate TPT degradation [8][9][10]. Furthermore, the protein expression of B. thuringiensis and its metabolic network related to the degradation of pollutants, such as erythromycin and triclosan, have been revealed by using omics approaches [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

Zhang

Qin

et al. 2019

Processes

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Triphenyltin (TPT) has severely polluted the environment, and it often coexists with metal ions, such as Cu2+. This paper describes the cell’s molecular recognition of TPT, the interaction between TPT recognition and Cu2+ biosorption, and their effect on cell permeability. We studied the recognition of TPT by Bacillus thuringiensis cells and the effect of TPT recognition on Cu2+ biosorption by using atomic force microscopy to observe changes in cell surface mechanical properties and cellular morphology and by using flow cytometry to determine the cell growth status and cell permeability. The results show that B. thuringiensis can quickly recognize different media. The adhesion force of cells in contact with Tween 80 was significantly reduced to levels that were much lower than that of cells in contact with PBS. Conversely, the cell surface adhesion force increased as TPT became more degraded. B. thuringiensis cells maintained their original morphology after 48 h of TPT treatment. The amount of Cu2+ adsorption by TPT-treated cells was positively correlated with the surface adhesion force (r = 0.966, P = 0.01). The cell adhesion force significantly decreased after Cu2+ adsorption, and cell recognition of TPT and/or Cu2+ hindered the entrance of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) into the cell. The initial diffusion time of DCFH-DA into cells treated by PBS, Cu2+, TPT, and TPT+Cu2+ was 4, 10, 30, and 30 min, respectively, and the order of the fluorescence intensity was PBS >> Cu2+ > TPT > TPT+Cu2+. We conclude that changes in the cell surface properties of the microbe during recognition of pollutants depend on the contaminant’s properties. B. thuringiensis recognized TPT and secreted intracellular substances that not only enhanced the adsorption of Cu2+, but also formed a “barrier” on the cell surface that reduced permeability. These findings provide a novel insight into the mechanism of microbial removal of pollutants.

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“…Biodegradation is a primary approach used to reduce TPT and its derivatives in a natural environment without exposure to ultraviolet radiation. Regarding the effective microbes for TPT degradation, Bacillus thuringiensis can effectively cleave the carbon-tin bonds of organotins and degrade organotins 4 , 5 . This spore-forming species was selected in the current study due to its use as a commonly used biopesticide without negative impacts on humans, wildlife, and pollinators 6 ; its TPT degradation ability; and its resistance to a variety of stresses, including pollutant toxicity.…”

Section: Introductionmentioning

confidence: 99%

Triphenyltin recognition by primary structures of effector proteins and the protein network of Bacillus thuringiensis during the triphenyltin degradation process

Wang

et al. 2017

Sci Rep

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Herein, triphenyltin (TPT) biodegradation efficiency and its transformation pathway have been elucidated. To better understand the molecular mechanism of TPT degradation, the interactions between amino acids, primary structures, and quaternary conformations of effector proteins and TPT were studied. The results verified that TPT recognition and binding depended on amino acid sequences but not on secondary, tertiary or quaternary protein structure. During this process, TPT could change the molecular weight and isoelectric point of effector proteins, induce their methylation or demethylation, and alter their conformation. The effector proteins, alkyl hydroperoxide reductase and acetyl-CoA acetyltransferase, recognizing TPT were crucial to TPT degradation. Electron transfer flavoprotein subunit alpha, phosphoenolpyruvate carboxykinase, aconitate hydratase, branched-chain alpha-keto acid dehydrogenase E1 component, biotin carboxylase and superoxide dismutase were related to energy and carbon metabolism, which was consistent with the results in vivo. The current findings develop a new approach for investigating the interactions between proteins and target compounds.

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Triphenyltin biosorption, dephenylation pathway and cellular responses during triphenyltin biodegradation by Bacillus thuringiensis and tea saponin

Cited by 27 publications

References 31 publications

Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny

Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny

Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

Triphenyltin recognition by primary structures of effector proteins and the protein network of Bacillus thuringiensis during the triphenyltin degradation process

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