Triphenyltin biodegradation and intracellular material release by Brevibacillus brevis

Ye, Jinshao; Zhao, Hangjian; Yin, Hua; Peng, Hui; Tang, Litao; Gao, Jiong; Ma, Yujia

doi:10.1016/j.chemosphere.2013.12.039

Cited by 12 publications

(4 citation statements)

References 30 publications

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“…Furthermore, the cell surface adhesion force was linearly positively correlated with the TPT, DPT, and MPT degradation rate (r = 0.948, p = 0.05; r = 0.972, p = 0.01; r = 0.967, p = 0.01; Table S2), which indicates that the higher the cell surface adhesion force, the stronger the degradation ability of individual cells. TPT is degraded intracellularly [23], which indirectly supports the hypothesis that intracellular secretions recognize TPT. This is further verified by the change in the adhesion force of B. thuringiensis during the degradation of TPT, DPT, and MPT (Figure 3b).…”

Section: Changes In Mechanical Properties Of Bacillus Thuringiensis During Biodegradation Of Phenyltinsupporting

confidence: 68%

“…The methanol solution had no obvious effect on the cell surface properties, but it did promote the recognition of TPT by the cells, which may be the result of the improved TPT solubility by methanol. TPT can induce cells to secrete intracellular substances [23] (mainly proteins) to resist the negative effect of TPT on cells. Therefore, TPT may be recognized by B. thuringiensis through extracellular secretions.…”

Section: Recognition and Binding Of Different Compoundsmentioning

confidence: 99%

“…It has been reported that TPT can be rapidly adsorbed by the cell surface through physicochemical interactions, such as electrostatic attraction, cell affinity, and ion exchange [24]. Then, TPT can easily penetrate the cell wall [9] and be transferred to the cytoplasm through active transport [25], stimulate the cell to secrete intracellular substances [23] (mainly proteins) to cover the cell surface, increase the cell surface adhesion force, and reduce the Young's modulus of the cell surface. According to the growth of the cells (Table S1) during TPT degradation, the cell biomass increased markedly in the first 12 h but slowly after 12 h, at which point it tended to be stable.…”

Section: Changes In Mechanical Properties Of Bacillus Thuringiensis During Biodegradation Of Phenyltinmentioning

confidence: 99%

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Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

Zhang

Qin

et al. 2019

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Triphenyltin (TPT) has severely polluted the environment, and it often coexists with metal ions, such as Cu2+. This paper describes the cell’s molecular recognition of TPT, the interaction between TPT recognition and Cu2+ biosorption, and their effect on cell permeability. We studied the recognition of TPT by Bacillus thuringiensis cells and the effect of TPT recognition on Cu2+ biosorption by using atomic force microscopy to observe changes in cell surface mechanical properties and cellular morphology and by using flow cytometry to determine the cell growth status and cell permeability. The results show that B. thuringiensis can quickly recognize different media. The adhesion force of cells in contact with Tween 80 was significantly reduced to levels that were much lower than that of cells in contact with PBS. Conversely, the cell surface adhesion force increased as TPT became more degraded. B. thuringiensis cells maintained their original morphology after 48 h of TPT treatment. The amount of Cu2+ adsorption by TPT-treated cells was positively correlated with the surface adhesion force (r = 0.966, P = 0.01). The cell adhesion force significantly decreased after Cu2+ adsorption, and cell recognition of TPT and/or Cu2+ hindered the entrance of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) into the cell. The initial diffusion time of DCFH-DA into cells treated by PBS, Cu2+, TPT, and TPT+Cu2+ was 4, 10, 30, and 30 min, respectively, and the order of the fluorescence intensity was PBS >> Cu2+ > TPT > TPT+Cu2+. We conclude that changes in the cell surface properties of the microbe during recognition of pollutants depend on the contaminant’s properties. B. thuringiensis recognized TPT and secreted intracellular substances that not only enhanced the adsorption of Cu2+, but also formed a “barrier” on the cell surface that reduced permeability. These findings provide a novel insight into the mechanism of microbial removal of pollutants.

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Section: Changes In Mechanical Properties Of Bacillus Thuringiensis During Biodegradation Of Phenyltinsupporting

confidence: 68%

Section: Recognition and Binding Of Different Compoundsmentioning

confidence: 99%

Section: Changes In Mechanical Properties Of Bacillus Thuringiensis During Biodegradation Of Phenyltinmentioning

confidence: 99%

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Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

Zhang

Qin

et al. 2019

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“…Brevibacillus brevis (formerly Bacillus brevis ), the type species of Brevibacillus , is considered a plant growth-promoting rhizobacterium (PGPR) ( 1 – 3 ) and organic pollutant degradation strain ( 4 , 5 ). B. brevis is widespread in the soil and sediment, and it has been widely used in agriculture and environmental remediation because of its multiple potential functions ( 6 , 7 ).…”

Section: Genome Announcementmentioning

confidence: 99%