Triphenyltetrazolium Reduction by Uterine Tissue of Rats.

Schultze, A. B.

doi:10.3181/00379727-116-29333

Cited by 7 publications

(3 citation statements)

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“…A direct effect of hormonal changes is the significant increase of uterine dehydrogenase activity on the 4th day of pregnancy (Schultze, 1964;Marchlewska-Koj, 1973). The data in Table 4 indicate that the 'stimulating males' have an influence on uterine enzyme activity in pregnant mice.…”

Section: Discussionmentioning

confidence: 98%

Male-pheromone effect on the efficiency of pregnancy in female mice

Marchlewska-Koj¹,

Jemioło²,

Woźniacka³

et al. 1980

Reproduction

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Outbred female mice were exposed during 3 oestrous cycles and mated to outbred or C57BL/kw males. Although ovulation number (CL and flushed eggs) was similar in all females, there were significantly fewer (P < 0.01) fertilized eggs and implantations in females exposed and mated to C57BL/kw males. Exposure to outbred males and mating with C57BL/kw males led to some improvement but the best results were obtained with females mated to outbred males. The activity of uterine dehydrogenases on Day 4 of pregnancy was also greatest in females mated to outbred males. It is concluded that the pheromonal stimulation leads to hormone concentration differences which affect implantation.

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Section: Discussionmentioning

confidence: 98%

Male-pheromone effect on the efficiency of pregnancy in female mice

Marchlewska-Koj¹,

Jemioło²,

Woźniacka³

et al. 1980

Reproduction

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“…The reaction is not promoted by progesterone, testosterone or cortisol. Schultze (1964) showed that, when uterine segments from spayed rats were incubated with ttc, the ttc was reduced to formazan at a rate proportional to the dosage of oestradiol administered. Furthermore, ttc reduction by uterine segments from intact cycling females was significantly higher at the pro-oestrous stage of the cycle than at other stages.…”

mentioning

confidence: 99%

“…Schultze weeks later. The method of assessing the reaction rate is described by Schultze (1964). Groups of twenty-six females aged 85 to 100 days were used.…”

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confidence: 99%

Litter Size and Proportion of Females in the Offspring of Multiparous Rats With Varying Uterine Metabolic Levels

Schultze¹

1965

Reproduction

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The rate of ttc reduction by the uterine tissue of rats was determined at specific stages of the oestrous cycle, and in successive cycles at the same stage. Individual females showed a characteristic level of reaction to ttc. Mothers whose uteri had a high reaction rate with ttc produced litters with a higher percentage of female pups than did mothers with a low level of reaction. Previous studies have shown that ttc reduction rate represents a metabolic activity of uterine tissue that is induced by oestrogens.

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Estradiol administration and reproductive performance in rats

Schultze

Ellicott

1968

J. Exp. Zool.

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Estradiol in doses of 2 and 4 micrograms increased uterine triphenyltetrazolium (TTC) metabolism at day of breeding when administered for two days prior to breeding. Estradiol as administered in these experiments resulted in decreased uterine TTC metabolism at day 4 and 8 of gestation when compared to the metabolism of untreated females.Two micrograms of estradiol administered one and two days prior to breeding increased reproductive performance. Two micrograms of estradiol administered one day prior to breeding and at day of breeding drastically reduced reproductive performance.It was the purpose of this study to determine the effect of slight hyperestrinism near the time of breeding on reproductive performance in rats. To evaluate the degree of hyperestrinism induced by different levels of exogenous estrogen, the rate of reduction of triphenyltetrazolium (TTC) by uterine segments in vitro was used. This uterine tissue reaction is associated with the effective estrogenic stimulation of the uterus in rats (Schultze, '64a,b). It is probable that a higher than normal estrogenic stimulation at early pregnancy may affect the estrogen status of the uterus at a stage later in pregnancy. Therefore, uterine TTC metabolism was determined in these experiments at several pregnancy stages following breeding and estrogen administration. METHODSRats of Wistar origin that have been maintained in this laboratory for several years were used. Virgin females between 110-120 days of age were assigned randomly to each of four treatment groups. Group 1 females received injection of 0.2 ml olive oil on the second day of diestrus and again on the following day at proestrus prior to breeding. These females are designated as controls. Group 2 females received injections of 2 vg estradiol 17B in 0.2 ml oil on the second day of diestrus and again on the following day at proestrus prior to breeding. These females are designated as the 2 clg-diestrus-proestrus group. Group 3 females were treated similar to group 2 females except that 4 vg J. EXP. ZOOL., 168: 531-534. estradiol instead of 2 pg estradiol were administered. These females are designated as the 4 vg-diestrus-proestrus group. Group 4 females received injections of 2 clg estradiol on day of proestrus and again at day of breeding. These females are designated as the 2 pg-proestrus-estrus group.Each female of the four groups was placed with a male of known fertility on the second day of diestrus. Breeding was confirmed by observation of sperm in the vaginal smear. If sperm were not found, the female was not included in the experiment. The day at which sperm were observed is designated as day 0 of pregnancy.Within each of the four treatment groups, females were sacrificed at day 0, 4, 8, 12 and 16 of pregnancy. Segments of uterine tissue were removed and rate of reduction of TTC by this tissue was determined at several stages. The method used has previously been described (Schultze, '64a). In addition to the TTC reduction the corpora lutea of rats killed at days 4, 8, 12 and 1...

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Triphenyltetrazolium Reduction by Uterine Tissue of Rats.

Abstract: The recent availability( 1,2) of 2-a-tocoph-on fetal resorption(3) indicated that the 2 erolt (2 4' d, 8' d-a-tocopherol JDistillation Products Industries/) has made possible the direct determination of the biopotency of this synthetic epimer of vitamin E. A report based

Cited by 7 publications

References 1 publication

Male-pheromone effect on the efficiency of pregnancy in female mice

Male-pheromone effect on the efficiency of pregnancy in female mice

Litter Size and Proportion of Females in the Offspring of Multiparous Rats With Varying Uterine Metabolic Levels

Estradiol administration and reproductive performance in rats

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