Tripartite relationship between gut microbiota, intestinal mucus and dietary fibers: towards preventive strategies against enteric infections

Sauvaitre, Thomas; Etienne‐Mesmin, Lucie; Sivignon, Adeline; Mosoni, Pascale; Courtin, Christophe M.; Wiele, Tom Van de; Blanquet‐Diot, Stéphanie

doi:10.1093/femsre/fuaa052

Cited by 32 publications

(28 citation statements)

References 444 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We argue that this positive effect on pathogen growth may not be an issue in the context of the complex nutritional and microbial background of the distal small intestine, the main site of ETEC colonization [ 3 , 4 , 86 , 87 , 88 , 89 ]. In the human gut, fibers are degraded into smaller carbohydrates by the endogenous gut microbiota, providing substrates for pathogens, such as ETEC, which generally behave as secondary degraders [ 90 ]. By performing fecal batch experiments including microbiota from human origin, we confirmed that dietary-fiber-containing products had no significant effect on ETEC colonization in a complex microbial environment, with only a slight tendency of yeast cell walls to reduce pathogen levels in both the luminal and mucosal compartments.…”

Section: Discussionmentioning

confidence: 99%

Lentils and Yeast Fibers: A New Strategy to Mitigate Enterotoxigenic Escherichia coli (ETEC) Strain H10407 Virulence?

et al. 2022

Self Cite

View full text Add to dashboard Cite

Dietary fibers exhibit well-known beneficial effects on human health, but their anti-infectious properties against enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is a major food-borne pathogen that causes acute traveler’s diarrhea. Its virulence traits mainly rely on adhesion to an epithelial surface, mucus degradation, and the secretion of two enterotoxins associated with intestinal inflammation. With the increasing burden of antibiotic resistance worldwide, there is an imperious need to develop novel alternative strategies to control ETEC infections. This study aimed to investigate, using complementary in vitro approaches, the inhibitory potential of two dietary-fiber-containing products (a lentil extract and yeast cell walls) against the human ETEC reference strain H10407. We showed that the lentil extract decreased toxin production in a dose-dependent manner, reduced pro-inflammatory interleukin-8 production, and modulated mucus-related gene induction in ETEC-infected mucus-secreting intestinal cells. We also report that the yeast product reduced ETEC adhesion to mucin and Caco-2/HT29-MTX cells. Both fiber-containing products strengthened intestinal barrier function and modulated toxin-related gene expression. In a complex human gut microbial background, both products did not elicit a significant effect on ETEC colonization. These pioneering data demonstrate the promising role of dietary fibers in controlling different stages of the ETEC infection process.

show abstract

Section: Discussionmentioning

confidence: 99%

Lentils and Yeast Fibers: A New Strategy to Mitigate Enterotoxigenic Escherichia coli (ETEC) Strain H10407 Virulence?

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The beneficial effects of dietary fibers on human health is now well acknowledged, but their ability to exert antagonistic effects against enteric pathogens remain poorly studied [7,36,[45][46][47]. To date, the vast majority of studies investigating the potential of fibers in the fight against ETEC-associated infections have been performed on porcine ETEC strains [48][49][50][51][52], while studies involving ETEC strains from human origins are scarce [28,29,53].…”

Section: Discussionmentioning

confidence: 99%

“…Insoluble dietary fiber particles have even been recently shown to constitute a microbiota niche on their own [5,6]. Another understudied effect of dietary fibers is their ability to prevent enteric infections [7]. Scarce in vitro studies have already shown the antagonistic properties of fibers against various enteric bacterial pathogens, mostly through a direct bacteriostatic effect, anti-adhesion properties on intestinal cells [8][9][10][11] or a decoy for pathogen/toxin binding to mucosal polysaccharides [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Dietary Fiber Anti-Infectious Properties against Food-Borne Enterotoxigenic Escherichia coli

et al. 2021

Self Cite

View full text Add to dashboard Cite

Dietary fibers have well-known beneficial effects on human health, but their anti-infectious properties against human enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is the main agent of travelers’ diarrhea, against which targeted preventive strategies are currently lacking. ETEC pathogenesis relies on multiple virulence factors allowing interactions with the intestinal mucosal layer and toxins triggering the onset of diarrheal symptoms. Here, we used complementary in vitro assays to study the antagonistic properties of eight fiber-containing products from cereals, legumes or microbes against the prototypical human ETEC strain H10407. Inhibitory effects of these products on the pathogen were tested through growth, toxin production and mucus/cell adhesion inhibition assays. None of the tested compounds inhibited ETEC strain H10407 growth, while lentil extract was able to decrease heat labile toxin (LT) concentration in culture media. Lentil extract and specific yeast cell walls also interfered with ETEC strain H10407 adhesion to mucin beads and human intestinal cells. These results constitute a first step in the use of dietary fibers as a nutritional strategy to prevent ETEC infection. Further work will be dedicated to the study of fiber/ETEC interactions within a complex gut microbial background.

show abstract

“…109 Increased availability of these free sugars in the intestinal lumen is made possible by the presence of saccharolytic Bacteroides thetaiotaomicron, on which C difficile and S typhimurium rely. 110 By the preceding, exploiting these molecules derived from the microbiota as both signals and nutrients remains critical for the hosts' pathogenic bacterial infection. Although such organisms have developed many strategies of circumventing the resistance to colonization given by the microbiota, and in other situations even employ its help, the microbiota offers repulsion and thus creates intense competition for resources.…”

Section: Detrimental Effects Of the Gut Microbiome In Inducing Colitismentioning

confidence: 99%

Significance of African Diets in Biotherapeutic Modulation of the Gut Microbiome

Isibor

Akinduti

Aworunse

et al. 2021

Bioinform Biol Insights

View full text Add to dashboard Cite

Diet plays an essential role in human development and growth, contributing to health and well-being. The socio-economic values, cultural perspectives, and dietary formulation in sub-Saharan Africa can influence gut health and disease prevention. The vast microbial ecosystems in the human gut frequently interrelate to maintain a healthy, well-coordinated cellular and humoral immune signalling to prevent metabolic dysfunction, pathogen dominance, and induction of systemic diseases. The diverse indigenous diets could differentially act as biotherapeutics to modulate microbial abundance and population characteristics. Such modulation could prevent stunted growth, malnutrition, induction of bowel diseases, attenuated immune responses, and mortality, particularly among infants. Understanding the associations between specific indigenous African diets and the predictability of the dynamics of gut bacteria genera promises potential biotherapeutics towards improving the prevention, control, and treatment of microbiome-associated diseases such as cancer, inflammatory bowel disease, obesity, type 2 diabetes, and cardiovascular disease. The dietary influence of many African diets (especially grain-base such as millet, maize, brown rice, sorghum, soya, and tapioca) promotes gut lining integrity, immune tolerance towards the microbiota, and its associated immune and inflammatory responses. A fibre-rich diet is a promising biotherapeutic candidate that could effectively modulate inflammatory mediators’ expression associated with immune cell migration, lymphoid tissue maturation, and signalling pathways. It could also modulate the stimulation of cytokines and chemokines involved in ensuring balance for long-term microbiome programming. The interplay between host and gut microbial digestion is complex; microbes using and competing for dietary and endogenous proteins are often attributable to variances in the comparative abundances of Enterobacteriaceae taxa. Many auto-inducers could initiate the process of quorum sensing and mammalian epinephrine host cell signalling system. It could also downregulate inflammatory signals with microbiota tumour taxa that could trigger colorectal cancer initiation, metabolic type 2 diabetes, and inflammatory bowel diseases. The exploitation of essential biotherapeutic molecules derived from fibre-rich indigenous diet promises food substances for the downregulation of inflammatory signalling that could be harmful to gut microbiota ecological balance and improved immune response modulation.

show abstract

Tripartite relationship between gut microbiota, intestinal mucus and dietary fibers: towards preventive strategies against enteric infections

Cited by 32 publications

References 444 publications

Lentils and Yeast Fibers: A New Strategy to Mitigate Enterotoxigenic Escherichia coli (ETEC) Strain H10407 Virulence?

Lentils and Yeast Fibers: A New Strategy to Mitigate Enterotoxigenic Escherichia coli (ETEC) Strain H10407 Virulence?

In Vitro Evaluation of Dietary Fiber Anti-Infectious Properties against Food-Borne Enterotoxigenic Escherichia coli

Significance of African Diets in Biotherapeutic Modulation of the Gut Microbiome

Contact Info

Product

Resources

About