Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality

Vilagran, Ingrid; Castillo‐Martín, Miriam; Prieto-Martínez, Noelia; Bonet, Sergi; Yeste, Marc

doi:10.1016/j.anireprosci.2015.12.001

Cited by 30 publications

(28 citation statements)

References 34 publications

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“…Novak, Ruiz‐Sánchez, Dixon, Foxcroft, and Dyck () observed that GPx in seminal plasma was correlated with farrowing rates together with triosephosphate isomerase (TPI) activity. In addition, epididymal GPx5 was proposed as quality marker of boar spermatozoa Vilagran, Castillo‐Martín, Prieto‐Martínez, Bonet, & Yeste, );. However, GPx5 was not able to predict boar ejaculate freezability as its levels did not correlate with principal component analysis of frozen‐thawed semen (Vilagran et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, epididymal GPx5 was proposed as quality marker of boar spermatozoa Vilagran, Castillo‐Martín, Prieto‐Martínez, Bonet, & Yeste, );. However, GPx5 was not able to predict boar ejaculate freezability as its levels did not correlate with principal component analysis of frozen‐thawed semen (Vilagran et al., ). Nonetheless, a stable production in SP and spermatozoa during the year is a positive characteristic for the application of GPx as a molecular marker for semen quality.…”

Section: Discussionmentioning

confidence: 99%

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Effects of season on boar semen parameters and antioxidant enzymes in the south subtropical region in Brazil

et al. 2018

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Although boar semen productivity is affected by seasonality, its effects are not equal among different regions which raise concerns regarding the profitability of boar stud farms. Therefore, the goals of this study were (i) to evaluate the seasonal effect on semen production in a commercial boar stud farm located in a subtropical climate region and (ii) to verify whether the activities of superoxide dismutase and glutathione peroxidase in spermatozoa and seminal plasma were associated with seminal traits of fresh and cooled semen. Nine boars were collected twice per season, and routine seminal parameter analyses were performed together with superoxide dismutase and glutathione peroxidase activities in seminal plasma and spermatozoa. Despite a reduction in sperm concentration in spring and summer, most seminal parameters were constant year-round. Temperature-humidity index was higher in the summer compared to spring, autumn and winter (p< .05). Superoxide dismutase activity in spermatozoa was increased in summer compared to autumn and winter (p < .05). The activities of both enzymes in seminal plasma and spermatozoa glutathione peroxidase remained unaltered throughout the seasons. In conclusion, seasonality showed little influence in overall boar seminal parameters despite microclimatic differences among seasons, and spermatozoa collected during summer increased superoxide dismutase activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of season on boar semen parameters and antioxidant enzymes in the south subtropical region in Brazil

et al. 2018

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“…It is a glycolytic enzyme that plays a crucial role in sperm metabolism and leads to the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (Wierenga et al, 2010). Sun et al (2018) pro led the sperm of mice and identi ed triosephosphate isomerase suggesting its role in sperm motility and being an important enzyme of glycolytic pathway that provides ATP required for motility of the sperm (Danshina et al, 2010;Vilagran et al, 2016). Previous studies demonstrated that TPI is located in the sperm head and is involved in sperm binding to zona pellucida (Auer et al, 2004;Petit et al, 2013).…”

Section: Metabolismmentioning

confidence: 99%

A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland

Saraswathi¹,

B.S²,

Kiran³

et al. 2020

Preprint

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Male accessory gland (MAG) proteins that are secretory in nature delivered to females, along with sperms affects female reproductive physiology and behavior. In our study, proteomic approaches were employed to identify the MAG proteins of L. orbonalis, a monophagous and most destructive pest on brinjal. A set of 117 spots in virgin MAG and 186 in mated MAG were obtained from 2-D gel electrophoresis. The differentially expressed MAG proteins after mating in comparison with the virgin male were 14 upregulated and 16 down-regulated. We have used MALDI- MS to identify the 13 unique proteins within the virgin MAG of the L. orbonalis and analyzed with the Swiss-Prot database using a Mascot search engine. The proteins were identified as proteolysis regulators, lipid transporter, olfactory protein, metabolism, DNA binding, and hexamerins. This is the first report on proteome analysis of MAG of the Leucinodes orbonalis.

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“…For example, the amount of enolase (ENO1) in bull spermatozoa was previously reported as a positive marker of bull sperm fertility (Park et al 2012) and its abundance in seminal plasma is positively associated with ram sperm preservation during liquid storage, but it is negatively correlated with ram sperm freezing success (Table 6). In the boar, the amount of triosephosphate isomerase (TPI) was negatively correlated with sperm membrane integrity, morphology and motility (Vilagran et al 2016), the ability of boar spermatozoa to survive cryopreservation (Vilagran et al 2013) and litter size following artificial insemination (Kwon et al 2015). Such differences could be species related or due to the measurement of TPI in spermatozoa compared to seminal plasma.…”

Section: Metabolism-related Proteins Correlated With Ram Sperm Functimentioning

confidence: 99%

Ram seminal plasma and its functional proteomic assessment

Leahy¹,

Rickard²,

Bernecic³

et al. 2019

Reproduction

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Ejaculation results in the confluence of epididymal spermatozoa with secretions of the accessory sex glands. This interaction is not a prerequisite for fertilisation success, but seminal factors do play a crucial role in prolonging the survival of spermatozoa both in vitro and in vivo by affording protection from handling induced stress and some selective mechanisms of the female reproductive tract. Reproductive biologists have long sought to identify specific factors in seminal plasma that influence sperm function and fertility in these contexts. Many seminal plasma proteins have been identified as diagnostic predictors of sperm function and have been isolated and applied in vitro to prevent sperm damage associated with the application of artificial reproductive technologies. Proteomic assessment of the spermatozoon, and its surroundings, has provided considerable advances towards these goals and allowed for greater understanding of their physiological function. In this review, the importance of seminal plasma will be examined through a proteomic lens to provide comprehensive analysis of the ram seminal proteome and detail the use of proteomic studies that correlate seminal plasma proteins with ram sperm function and preservation ability.

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Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality

Cited by 30 publications

References 34 publications

Effects of season on boar semen parameters and antioxidant enzymes in the south subtropical region in Brazil

Effects of season on boar semen parameters and antioxidant enzymes in the south subtropical region in Brazil

A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland

Ram seminal plasma and its functional proteomic assessment

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