Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish

Chen, Jiangfei; Huang, Changjiang; Zheng, Lidan; Simonich, Michael T.; Bai, Chenglian; Tanguay, Robert L.; Dong, Qiaoxiang

doi:10.1016/j.ntt.2011.09.003

Cited by 53 publications

(27 citation statements)

References 40 publications

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“…Low levels of acute and sub-chronic chlorpyrifos (≤1 μM) and its oxon form (≤1 nM) elicited neurotoxicity without affecting AChE and modify axonal and dendritic outgrowth in neuronal cultures, which is concentration dependent (Howard et al, 2005). Along similar lines, chlorpyrifos administration (at levels not inhibiting AChE) in rodent models indicates that anxiety behavior was modified, yet results were conflicting (Chen et al, 2011; Ricceri et al, 2006; Venerosi et al, 2010). In the present study, observed changes in anxiety-related behavior suggest that chlorpyrifos promotes neurotoxicity, thereby damaging neural patterning and circuitry in early brain development, at doses that do not alter AChE levels.…”

Section: Discussionmentioning

confidence: 96%

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Richendrfer¹,

Pelkowski²,

Colwill³

et al. 2012

Neurotoxicology and Teratology

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Neurobehavioral disorders such as anxiety, autism, and attention deficit hyperactivity disorders are typically influenced by genetic and environmental factors. Although several genetic risk factors have been identified in recent years, little is known about the environmental factors that either cause neurobehavioral disorders or contribute to their progression in genetically predisposed individuals. One environmental factor that has raised concerns is chlorpyrifos, an organophosphate pesticide that is widely used in agriculture and is found ubiquitously in the environment. In the present study, we examined the effects of sub-chronic chlorpyrifos exposure on anxiety-related behavior during development using zebrafish larvae. We found that sub-chronic exposure to 0.01 or 0.1 μM chlorpyrifos during development induces specific behavioral defects in 7-day-old zebrafish larvae. The larvae displayed decreases in swim speed and thigmotaxis, yet no changes in avoidance behavior were seen. Exposure to 0.001 μM chlorpyrifos did not affect swimming, thigmotaxis, or avoidance behavior and exposure to 1 μM chlorpyrifos induced behavioral defects, but also induced defects in larval morphology. Since thigmotaxis, a preference for the edge, is an anxiety-related behavior in zebrafish larvae, we propose that sub-chronic chlorpyrifos exposure interferes with the development of anxiety-related behaviors. The results of this study provide a good starting point for examination of the molecular, cellular, developmental, and neural mechanisms that are affected by environmentally relevant concentrations of organophosphate pesticides. A more detailed understanding of these mechanisms is important for the development of predictive models and refined health policies to prevent toxicant-induced neurobehavioral disorders.

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Section: Discussionmentioning

confidence: 96%

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Richendrfer¹,

Pelkowski²,

Colwill³

et al. 2012

Neurotoxicology and Teratology

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“…For example, metal oxide nanoparticles (CuO, ZnO, Cr 2 O 3 , and NiO) inhibited ZHE1 activity (Lin et al, 2013). In addition, trimethyltin chloride and perfluorooctanesulfonic acid stimulated the spontaneous tail movements at 20 and 25 hpf, respectively, however time to hatch was not determined (Chen et al, 2011; Huang et al, 2010a). Ultimately, changes in time to hatch are suggested to have long term effects on embryo fitness (Danzmann et al, 1989; Pakkasmaa and Jones, 2002).…”

Section: Discussionmentioning

confidence: 97%

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

et al. 2014

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In the aquatic environment, adverse outcomes from dietary polycyclic aromatic hydrocarbon (PAH) exposure are poorly understood, and multigenerational developmental effects following exposure to PAHs are in need of exploration. Benzo[a]pyrene (BaP), a model PAH, is a recognized carcinogen and endocrine disruptor. Here adult zebrafish (F0) were fed 0, 10, 114, or 1012 μg BaP/g diet at a feed rate of 1% body weight twice/day for 21 days. Eggs were collected and embryos (F1) were raised to assess mortality and time to hatch at 24, 32, 48, 56, 72, 80, and 96 hours post fertilization (hpf) before scoring developmental deformities at 96 hpf. F1 generation fish were raised to produce the F2 generation followed by the F3 and F4 generations. Mortality significantly increased in the higher dose groups of BaP (2.3 and 20 μg BaP/g fish) in the F1 generation while there were no differences in the F2, F3, or F4 generations. In addition, premature hatching was observed among the surviving fish in the higher dose of the F1 generation, but no differences were found in the F2 and F3 generations. While only the adult F0 generation was BaP-treated, this exposure resulted in multigenerational phenotypic impacts on at least two generations (F1 and F2). Body morphology deformities (shape of body, tail, and pectoral fins) were the most severe abnormality observed, and these were most extreme in the F1 generation but still present in the F2 but not F3 generations. Craniofacial structures (length of brain regions, size of optic and otic vesicles, and jaw deformities), although not significantly affected in the F1 generation, emerged as significant deformities in the F2 generation. Future work will attempt to molecularly anchor the persistent multigenerational phenotypic deformities noted in this study caused by BaP exposure.

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“…Embryos derived from PFOS chronic exposed parents were allowed to develop in the absence of PFOS, and surviving larvae at 4 dpf with normal morphology were further subjected to a behavioral assessment in response to alternating light and dark stimulus as described earlier (Chen et al 2011). The test was performed in a ZebraLab behavior monitoring station (ViewPoint Life Sciences, Inc. Montreal, Canada).…”

Section: Methodsmentioning

confidence: 99%

Chronic PFOS exposures induce life stage–specific behavioral deficits in adult zebrafish and produce malformation and behavioral deficits in F1 offspring

Chen

Das

et al. 2012

Enviro Toxic and Chemistry

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Abstract-Perfluorooctane sulfonic acid (PFOS) is an organic contaminant that is ubiquitous in the environment. Few studies have assessed the behavioral effects of chronic PFOS exposure in aquatic organisms. The present study defined the behavioral effects of varying life span chronic exposures to PFOS in zebrafish. Specifically, zebrafish were exposed to control or 0.5 mM PFOS during 1 to 20, 21 to 120, or 1 to 120 d postfertilization (dpf). Exposure to PFOS impaired the adult zebrafish behavior mode under the tapping stimulus. The movement speed of male and female fish exposed for 1 to 120 dpf was significantly increased compared with control before and after tapping, whereas in the groups exposed for 1 to 20 and 21 to 120 dpf, only the males exhibited elevated swim speed before tapping. Residues of PFOS in F1 embryos derived from parental exposure for 1 to 120 and 21 to 120 dpf were significantly higher than control, and F1 embryos in these two groups also showed high malformation and mortality. The F1 larvae of parental fish exposed to PFOS for 1 to 20 or 21 to 120 dpf exhibited a higher swimming speed than control larvae in a light-to-dark behavior assessment test. The F1 larvae derived from parental fish exposed to PFOS for 1 to 120 dpf showed a significantly lower speed in the light period and a higher speed in the dark period compared with controls. Although there was little PFOS residue in embryos derived from the 1-to 20-dpf parental PFOSexposed group, the adverse behavioral effects on both adult and F1 larvae indicate that exposure during the first 21 dpf induces long-term neurobehaviorial toxicity. The authors' findings demonstrate that chronic PFOS exposure during different life stages adversely affects adult behavior and F1 offspring morphology, behavior, and survival. Environ. Toxicol. Chem. 2013;32:201-206. # 2012 SETAC

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Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish

Cited by 53 publications

References 40 publications

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

Chronic PFOS exposures induce life stage–specific behavioral deficits in adult zebrafish and produce malformation and behavioral deficits in F1 offspring

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