Trimethylsilylation reaction of prostaglandin-E methyl ester with various trimethylsilylating reagents

Derivatization in Mass Spectrometry—1. Silylation

“…For example, attempts have been made to completely silylate a carbonyl group in addition to other hydroxyl groups in prostaglandins. 54 However, the use of various silylating mixtures, for example, TMSIM in piperidine or pyridine and BSTFA in piperidine or pyridine, yielded mixtures of products. In addition, enol-TMS ethers are thermally and hydrolytically unstable.…”

Silyl Derivatization of Alkylphenols, Chlorophenols, and Bisphenol A for Simultaneous GC/MS Determination

“…Although the APEs are less toxic to organisms, their metabolites , show high toxicity to organisms and fish. , So most countries have classified them as endocrine disrupter chemicals. − Numerous papers have been published about determination of APs, CPs, and BPA using various techniques. Some of the most frequently used methods for analysis of these are as follows: direct analysis using HPLC, − GC/ECD, − LC/MS, GC/MS, , and other techniques , or indirect analysis of the compounds using derivatization techniques such as methylation, acetylation, , and silylation. − Methylation and acetylation techniques are suitable for the analytes having high molecular weights rather than small. For the phenolic analytes concerned in this work, significant loss may occur during the sample preparation process due to the high volatility of low molecular weight methyl esters or acetates of analytes …”

“…Some of the most frequently used methods for analysis of these are as follows: direct analysis using HPLC, 12-14 GC/ECD, 15-17 LC/MS, 18 GC/MS, 19,20 and other techniques 21,22 or indirect analysis of the compounds using derivatization techniques such as methylation, 23 acetylation, 24,25 and silylation. [26][27][28] Methylation and acetylation techniques are suitable for the analytes * To whom correspondence should be addressed: (phone)…”