Trimethylated chitosans as non-viral gene delivery vectors: Cytotoxicity and transfection efficiency

Kean, Thomas J.; Roth, Susanne; Thanou, Maya

doi:10.1016/j.jconrel.2005.01.001

Cited by 358 publications

(175 citation statements)

References 34 publications

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“…However, a high degree of trimethylation (94%) of the same low molecular weight chitosan lowered IC 50 to 1.4 mg/mL (MCF7) and 2.2 mg/mL (COS7) (Kean et al, 2005).…”

Section: Tmc Toxicitymentioning

confidence: 99%

“…However, with respect to another positively charged polymer, polyethyleneimine (PEI), TMC showed much lower toxicity (Kean et al, 2005). TMC, despite some toxicity related to free cationic charges, has therefore a key role to play in wound healing as a drug delivery material, most likely when complexed with anionic drugs such as RNA or DNA.…”

Section: Tmc Toxicitymentioning

confidence: 99%

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Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

435

196

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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“…However, a high degree of trimethylation (94%) of the same low molecular weight chitosan lowered IC 50 to 1.4 mg/mL (MCF7) and 2.2 mg/mL (COS7) (Kean et al, 2005).…”

Section: Tmc Toxicitymentioning

confidence: 99%

Section: Tmc Toxicitymentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

435

196

View full text Add to dashboard Cite

show abstract

“…CS is a well-known naturally occurring molecule which has been widely used as a gene/ drug nanocarrier and for the functionalization of nanoparticles in order to improve their aqueous dispersibility 58 . It has also been considered a promising alternative to PEI as it exhibits less cytotoxicity 59 . However, Bao et al reported much lower transfection efficacy with GO-CS:pDNA nanocomplexes compared to those based on PEI:pDNA alone, implying that the NA transfer efficiency of such vectors is yet to be optimized 56 .…”

Section: Strategies To Optimize Gbms As Gene Delivery Platformmentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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“…Lastly the paper produced with chitosan has a smoother surface and is more resistant to moisture. Among other things, chitosan is of great value in the production of toilet paper and for wrapping paper and cardboard [10,11].…”

Section: Introductionmentioning

confidence: 99%

Enhance the Quality of Cellulosic Fibers Produced from Agricultural Wastes through Flocculation Using Chitosan

Mohamed¹,

Nagieb

Sabour³

2017

Egypt. J. Chem.

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A PAPER sheets were prepared by using different types of cellulosic fibers produced from agricultural wastes. Chitosan solutions with different concentrations were added to the surface of paper sheets. Mechanical properties enhanced in case of treated paper sheets with chitosan comparing with untreated. Generally, in all cases of paper sheets prepared from different pulps, the strength properties reached their optimum enhancement in presence of 0.5% chitosan solution almost. Physical properties enhanced by addition of chitosan. Paper sheets prepared from unbleached bagasse treated with chitosan gave the best results compared with that prepared from the others. Also, paper sheets prepared from unbleached rice straw and that prepared from unbleached mixed (rice straw and 25% bagasse) gave impressive results. Crystallinity index from the ratio A1435cm-1/ A 900 cm-1 in Infrared spectra proved all the above. It was observed from the morphology of fibers that fibers of the sized paper enhanced and became tight and smooth.

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Trimethylated chitosans as non-viral gene delivery vectors: Cytotoxicity and transfection efficiency

Cited by 358 publications

References 34 publications

Chitosan as a starting material for wound healing applications

Chitosan as a starting material for wound healing applications

Graphene materials as 2D non-viral gene transfer vector platforms

Enhance the Quality of Cellulosic Fibers Produced from Agricultural Wastes through Flocculation Using Chitosan

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