Trimellitic anhydride facilitates transepithelial permeability disrupting tight junctions in sinonasal epithelial cells

Ogi, Kazuhiro; Li, Sha; Ramezanpour, Mahnaz; Cooksley, Clare; Javadiyan, Shari; Fujieda, Shigeharu; Wormald, Peter‐John; Vreugde, Sarah; Psaltis, Alkis James

doi:10.1016/j.toxlet.2021.09.016

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Although a higher concentration of TMA would involve a higher concentration of DMSO, it might lead to a more robust response from the test cells. A previous study showed that TMA disrupts the barrier function of primary human nasal epithelial cells at concentrations of ≥ 2 mg/ml, 50 which is at least eightfold greater than the concentration in our study.…”

Section: Discussionmentioning

confidence: 96%

“…TMA is a common chemical that is used as a curing agent. 50 Its interactions with human serum albumin lead to the formation of hapten-protein complexes and the onset of occupational asthma. [51][52][53] The negative response to TMA implies either that TMA itself is not immunogenic, or that the exposure concentration was insufficient.…”

Section: The Effect Of Hbtu Tbtu and Hdi On Cd54 Expressionmentioning

confidence: 99%

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Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

Tanabe,

Yoshida,

Ishikawa

et al. 2023

Altern Lab Anim

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Chemical respiratory sensitisation is a serious health problem. However, to date, there are no validated test methods available for identifying respiratory sensitisers. The aim of this study was to develop an in vitro sensitisation test by modifying the human cell line activation test (h-CLAT) to detect respiratory sensitisers and distinguish them from skin sensitisers. THP-1 cells were exposed to the test chemicals (two skin sensitisers and six respiratory sensitisers), either as monocultures or as cocultures with air–liquid interface-cultured reconstructed human bronchial epithelium. The responses were analysed by measuring the expression levels of surface markers on THP-1 cells (CD86, CD54 and OX40L) and the concentrations of cytokines in the culture media (interleukin (IL)-8, IL-33 and thymic stromal lymphopoietin (TSLP)). The cocultures exhibited increased CD54 expression on THP-1 cells; moreover, in the cocultures but not in the monocultures, exposure to two uronium salts (i.e. respiratory sensitisers) increased CD54 expression on THP-1 cells to levels above the criteria for a positive h-CLAT result. Additionally, exposure to the respiratory sensitiser abietic acid, significantly increased IL-8 concentration in the culture medium, but only in the cocultures. Although further optimisation of the method is needed to distinguish respiratory from skin sensitisers by using these potential markers (OX40L, IL-33 and TSLP), the coculture of THP-1 cells with bronchial epithelial cells offers a potentially useful approach for the detection of respiratory sensitisers.

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Section: Discussionmentioning

confidence: 96%

Section: The Effect Of Hbtu Tbtu and Hdi On Cd54 Expressionmentioning

confidence: 99%

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

Tanabe,

Yoshida,

Ishikawa

et al. 2023

Altern Lab Anim

View full text Add to dashboard Cite

show abstract

“…TJ exists in the apical region of the airway epithelium and provides initial protection against allergens, pathogens, and chemical stress [60]. The maintenance of TJ in nasal epithelial cells depends on expressions of TJ comprising transmembrane proteins, such as zonula occludes, occludin, claudin, and JAM [7,61].…”

Section: Discussionmentioning

confidence: 99%

Abies holophylla Leaf Essential Oil Alleviates Allergic Rhinitis Based on Network Pharmacology

et al. 2023

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Abies holophylla is an evergreen coniferous species that has been widely used for treating pulmonary diseases and colds. Previous research has demonstrated the anti-inflammatory effect of Abies species and the anti-asthmatic activities of Abies holophylla leaf essential oil (AEO). As asthma and allergic rhinitis (AR) share pathophysiology and pharmacotherapeutic interventions, AEO inhalation can also ameliorate upper respiratory allergic diseases. This study explored the protective effects of AEO on AR with network pharmacological pathway prediction. The potential target pathways of AEO were analyzed by a network pharmacological approach. The BALB/c mice were sensitized by ovalbumin (OVA) and 10 μm particular matter (PM10) to induce allergic rhinitis. Aerosolized AEO 0.0003% and 0.03% were delivered by nebulizer for 5 min a day, 3 times a week for 7 weeks. Nasal symptoms (sneezing and rubbing), histopathological changes in nasal tissues, serum IgE, and zonula occludens-1 (ZO-1) expressions on nasal tissues were analyzed. After AR induction with OVA+PM10 and inhalation of AEO 0.0003% and 0.03% treatment, AEO significantly decreased allergic symptoms (sneezing and rubbing), hyperplasia of nasal epithelial thickness, goblet cell counts, and serum IgE level. The network analysis demonstrated that the possible molecular mechanism of AEO is highly associated with the IL-17 signaling pathway and tight junction. The target pathway of AEO was investigated in RPMI 2650 nasal epithelial cells. Treatment of AEO on PM10-treated nasal epithelial cells significantly reduced the production of inflammatory mediators related to the IL-17 signaling pathway, NF-κB, and the MAPK signaling pathway and prevented the reduction in TJ-related factors. When taken together, AEO inhalation may be considered as a potential treatment for AR by alleviating nasal inflammation and recovering the tight junction.

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Occupational Rhinitis: An Update

Zamora-Sifuentes

Poole

2023

Curr Allergy Asthma Rep

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Trimellitic anhydride facilitates transepithelial permeability disrupting tight junctions in sinonasal epithelial cells

Cited by 4 publications

References 34 publications

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

Abies holophylla Leaf Essential Oil Alleviates Allergic Rhinitis Based on Network Pharmacology

Occupational Rhinitis: An Update

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