TRIMe7-CypA, an alternative splicing isoform of TRIMCyp in rhesus macaque, negatively modulates TRIM5α activity

Lei, Na; Tang, Yan-Dong; Liu, Jiandong; Yu, Changqing; Sun, Liuke; Lin, Yuezhi; Wang, Xuefeng; Wang, Xiaojun; Zhou, Jianhua

doi:10.1016/j.bbrc.2014.02.132

Cited by 5 publications

(8 citation statements)

References 30 publications

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“…To investigate whether the equine Mx2 protein has the ability to restrict the replication of certain lentiviruses, and to compare its function with that of huMxB, we cloned these two genes into a pcDNA3.1(+) vector with two-hemagglutinin (2×HA) tags at the C terminus and expressed them in HEK293T cells. huMxA and rhesus macaque TRIM5α (rhTRIM5α) were also expressed as negative ( 1 , 23 ) and positive ( 28 – 30 ) controls, respectively ( Fig. 3A ).…”

Section: Resultsmentioning

confidence: 99%

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Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

Lei

Ren

et al. 2018

J Virol

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Previous research has shown that the antiviral ability of Mx2s is confined to primates, particularly humans. EIAV has been shown to be insensitive to restriction by human MxB. Here, we describe the function of equine Mx2. This protein plays an important role in the suppression of EIAV, HIV-1, and SIVs. The antiviral activity of eqMx2 depends on its subcellular location as well as its capsid binding capacity. Our results showed that following viral infection, eqMx2 changes its original cytoplasmic location and accumulates at the nuclear envelope, where it binds to the viral capsid and blocks the nuclear entry of reverse-transcribed proviral DNAs. In contrast, huMxB does not bind to the EIAV capsid and shows no EIAV restriction effect. These studies expand our understanding of the function of the equine Mx2 protein.

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Section: Resultsmentioning

confidence: 99%

“…All constructed mutants were confirmed by sequencing. Rhesus macaque TRIM5α (rhTRIM5α) was synthesized as previously described ( 30 ).…”

Section: Methodsmentioning

confidence: 99%

Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

Lei

Ren

et al. 2018

J Virol

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“…Pseudo-typed HIV-1 expressing GFP was prepared as shown before (71). For infections, HeLa cells stable transduced with TRIM5 genes of interest or empty vector were seeded at a density of 1.5 × 10 5 cells/well in a 24-well plate, incubated with this pseudo-typed virus (described above) for 48 hrs.…”

Section: Infection With Hiv-1 Expressing Gfpmentioning

confidence: 99%

Rhesus monkey TRIM5α protein SPRY domain contributes to AP-1 activation

Lei

Tang

Wang

et al. 2018

Journal of Biological Chemistry

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“…Despite this, however, the occurrence of drug resistance mutations (DRMs) can reduce viral susceptibility to antiretroviral drugs (ARVs). Cell culture experiments have often been predictive of HIV drug resistance pathways (1,2), but the development and persistence of DRMs is also governed by complex pharmacologic, viral, and host factors (1,3,4). Although animal models may allow for the direct study of DRMs and their effects on treatment success (1), there is no model that re-creates all aspects of HIV-1 infection in humans (5).…”

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confidence: 99%

“…The construction of simian-tropic HIV-1 (stHIV-1), with an 88% sequence homology to HIV-1 has been accomplished (5). By replacing the HIV-1 capsid and vif regions with the corresponding regions from SIV (2,5,6), this HIV-based chimera (stHIV-1 (SCA,SVIF) ) is capable of infecting both human and macaque cell lines by evading TRIM5␣ (tripartite motif-containing protein 5 alpha) and APOBEC3G (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G) restrictions (5).…”

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confidence: 99%

Simian-Tropic HIV as a Model To Study Drug Resistance against Integrase Inhibitors

Wares

Hassounah

Mésplède

et al. 2015

Antimicrob Agents Chemother

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Drug resistance represents a key aspect of human immunodeficiency virus (HIV) treatment failure. It is important to develop nonhuman primate models for studying issues of drug resistance and the persistence and transmission of drug-resistant viruses. However, relatively little work has been conducted using either simian immunodeficiency virus (SIV) or SIV/HIV recombinant viruses for studying resistance against integrase strand transfer inhibitors (INSTIs). Here, we used a T-cell-tropic SIV/HIV recombinant virus in which the capsid and vif regions of HIV-1 were replaced with their SIV counterparts (simian-tropic HIV-1 [stHIV-1] (SCA,SVIF) ) to study the impact of a number of drug resistance substitutions in the integrase coding region at positions E92Q, G118R, E138K, Y143R, S153Y, N155H, and R263K on drug resistance, viral infectivity, and viral replication capacity. Our results show that each of these substitutions exerted effects that were similar to their effects in HIV-1. Substitutions associated with primary resistance against dolutegravir were more detrimental to stHIV-1 (SCA,SVIF) infectiousness than were resistance substitutions associated with raltegravir and elvitegravir, consistent with data that have been reported for HIV-1. These findings support the role of stHIV-1 (SCA,SVIF) as a useful model with which to evaluate the role of INSTI resistance substitutions on viral persistence, transmissibility, and pathogenesis in a nonhuman primate model.

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TRIMe7-CypA, an alternative splicing isoform of TRIMCyp in rhesus macaque, negatively modulates TRIM5α activity

Cited by 5 publications

References 30 publications

Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

Rhesus monkey TRIM5α protein SPRY domain contributes to AP-1 activation

Simian-Tropic HIV as a Model To Study Drug Resistance against Integrase Inhibitors

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