TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer

Liu, Lei; Zhao, Enhong; Li, Chunhui; Huang, Liang; Xiao, Ling; Cheng, Luyang; Huang, Xu; Song, Yan; Xu, Dawei

doi:10.1016/j.canep.2012.08.005

Cited by 55 publications

(49 citation statements)

References 26 publications

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“…More recently, Liu et al [50] reported that TRIM28 is a possible marker for the detection of circulating cancer cells, and provided evidence for a role for TRIM28 in metastasis development and prognosis in early stage non–small-cell lung cancer (NSCLC). In this study, 35 of 48 patients with NSCLC had overexpressed TRIM28 mRNA levels, while the rest of these showed underexpressed TRIM28 mRNA levels, compared to non-cancerous tissue.…”

Section: Resultsmentioning

confidence: 99%

TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers

et al. 2014

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Malignant gliomas are among the rarest brain tumours, and they have the worst prognosis. Grade IV astrocytoma, known as glioblastoma multiforme (GBM), is a highly lethal disease where the standard therapies of surgery, followed by radiation and chemotherapy, cannot significantly prolong the life expectancy of the patients. Tumour recurrence shows more aggressive form compared to the primary tumour, and results in patient survival from 12 to 15 months only. Although still controversial, the cancer stem cell hypothesis postulates that cancer stem cells are responsible for early relapse of the disease after surgical intervention due to their high resistance to therapy. Alternative strategies for GBM therapy are thus urgently needed. Nanobodies are single-domain antigen-binding fragments of heavy-chain antibodies, and together with classical antibodies, they are part of the camelid immune system. Nanobodies are small and stable, and they share a high degree of sequence identity to the human heavy chain variable domain, and these characteristics offer them advantages over classical antibodies or antibody fragments. We first immunised an alpaca with a human GBM stem-like cell line prepared from primary GBM cultures. Next, a nanobody library was constructed in a phage-display vector. Using nanobody phage-display technology, we selected specific GBM stem-like cell binders through a number of affinity selections, using whole cell protein extracts and membrane protein-enriched extracts from eight different GBM patients, and membrane protein-enriched extracts from two established GBM stem-like cell lines (NCH644 and NCH421K cells). After the enrichment, periplasmic extract ELISA was used to screen for specific clones. These nanobody clones were recloned into the pHEN6 vector, expressed in Escherichia coli WK6, and purified using immobilised metal affinity chromatography and size-exclusion chromatography. Specific nanobody:antigen pairs were obtained and mass spectrometry analysis revealed two proteins, TRIM28 and β-actin, that were up-regulated in the GBM stem-like cells compared to the controls.

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Section: Resultsmentioning

confidence: 99%

TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers

et al. 2014

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“…10 KAP1 could be considered also a prognostic marker for non-small cell lung cancer. 11 Altered metabolism is one of the hallmarks of cancer cells. The best-known metabolic abnormality in cancer cells is the Warburg effect, which is an increased glycolysis even in the presence of oxygen.…”

Section: Introductionmentioning

confidence: 99%

High grade glioblastoma is associated with aberrant expression of ZFP57, a protein involved in gene imprinting, and of CPT1A and CPT1C that regulate fatty acid metabolism

Cirillo

Salle

Petillo

et al. 2014

Cancer Biology & Therapy

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The diagnosis of glioblastoma is still based on tumor histology, but emerging molecular diagnosis is becoming an important part of glioblastoma classification. Besides the well-known cell cycle-related circuitries that are associated with glioblastoma onset and development, new insights may be derived by looking at pathways involved in regulation of epigenetic phenomena and cellular metabolism, which may both be highly deregulated in cancer cells. We evaluated if in glioblastoma patients the high grade of malignancy could be associated with aberrant expression of some genes involved in regulation of epigenetic phenomena and lipid metabolism. We measured the mRNA levels of ZFP57, TRIM28, CPT1A, CPT1B, and CPT1C in a cohort of 80 patients divided in two groups: grade II and grade IV. We evidenced that high grade glioblastoma is associated with increased level of ZFP57, a protein involved in gene imprinting, and aberrant expression of CPT1A and CPT1C, regulators of fatty acid oxidation. Our study may pave the way to identify new markers that could be potentially useful for diagnosis and/or prognosis of glioblastoma.

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“…Abnormal expression or deletion of KAP1 has been implicated in several physiological and biochemical complications or diseases, such as, cancer development, impairment of learning and memory, etc. [9][10][11][14][15][16]. As KAP1 is vital for many cellular processes, it can be studied in different angles for the better understanding of cellular process.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, this protein has been reported to be up-regulated in different cancers, and implicated in the activation or repression of transcriptional process of several genes [9]. For example, upregulated KAP1 provides a survival advantage to gastric and lung cancer cells, and is speculated as a potential diagnostic marker of cancer metastasis [11,16].…”

Section: Introductionmentioning

confidence: 99%

Establishment of stable cell line for inducing KAP1 protein expression

Liu

Cheng

Wei

et al. 2015

Acta Biologica Hungarica

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Generation of the stable cell lines is a highly efficient tool in functional studies of certain genes or proteins, where the particular genes or proteins are inducibly expressed. The KRAB-associated protein-1 (KAP1) is an important transcription regulatory protein, which is investigated in several molecular biological studies. In this study, we have aimed to generate a stable cell line for inducing KAP1 expression. The recombinant plasmid pcDNA5/FRT/TO-KAP1 was constructed at first, which was then transfected into Flp-In TM T-REx TM -HEK293 cells to establish an inducible pcDNA5/FRT/TO-KAP1-HEK293 cell line. The Western blot analysis showed that the protein level of KAP1 is over-expressed in the established stable cell line by doxycycline induction, both dose and time dependently. Thus we have successfully established stable pcDNA5/FRT/TO-KAP1-HEK293 cell line, which can express KAP1 inducibly. This inducible cell line might be very useful for KAP1 functional studies.

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TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer

Cited by 55 publications

References 26 publications

TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers

TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers

High grade glioblastoma is associated with aberrant expression of ZFP57, a protein involved in gene imprinting, and of CPT1A and CPT1C that regulate fatty acid metabolism

Establishment of stable cell line for inducing KAP1 protein expression

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