TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma

Yang, Yanru; Zuo, Sai-Jie; Li, Wenqing; Di, Man; Liu, Jin; Chai, Jia; Wang, Jing Jing; Yuan, Yuan; Li, Mingyang; Jia, Qingge

doi:10.1016/j.prp.2023.154710

Cited by 1 publication

(2 citation statements)

References 26 publications

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“…Additionally, given the pivotal role of HLA genes in the immunotherapeutic responses, our study revealed a subtle positive correlation between the expression of all HLA genes and miR-484, implying that individuals with elevated miR-484 levels might be better suited to receive immunotherapy. Furthermore, in the context of CC, cancer stem cells, hypoxia, and RNA methylation significantly contribute to cell proliferation, metastasis, metabolism, and therapeutic resistance within the TME [25][26][27]42]. This current study identified significant differences in most stem cell markers and RNA methylation levels between the two subgroups.…”

Section: Discussionmentioning

confidence: 59%

“…Cancer stem cells, characterized by their capacity for self-renewal, uncontrolled proliferation, and initiation of tumorigenesis, are recognized as the main contributors to the progression, metastasis, and treatment resistance of CC [25]. In our study, the ssGSEA algorithm was utilized to quantify the enrichment scores of the 26 stemness genes.…”

Section: Mir-484 Expression Correlated With Tumor Stemness Hypoxia An...mentioning

confidence: 99%

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Exploring MiR-484 Regulation by Polyalthia longifolia: A Promising Biomarker and Therapeutic Target in Cervical Cancer through Integrated Bioinformatics and an In Vitro Analysis

Niu,

Chen,

Chai

et al. 2024

Biomedicines

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Background: MiR-484, implicated in various carcinomas, holds promise as a prognostic marker, yet its relevance to cervical cancer (CC) remains unclear. Our prior study demonstrated the Polyalthia longifolia downregulation of miR-484, inhibiting HeLa cells. This study investigates miR-484’s potential as a biomarker and therapeutic target in CC through integrated bioinformatics and an in vitro analysis. Methods: MiR-484 levels were analyzed across cancers, including CC, from The Cancer Genome Atlas. The limma R package identified differentially expressed genes (DEGs) between high- and low-miR-484 CC cohorts. We assessed biological functions, tumor microenvironment (TME), immunotherapy, stemness, hypoxia, RNA methylation, and chemosensitivity differences. Prognostic genes relevant to miR-484 were identified through Cox regression and Kaplan–Meier analyses, and a prognostic model was captured via multivariate Cox regression. Single-cell RNA sequencing determined cell populations related to prognostic genes. qRT-PCR validated key genes, and the miR-484 effect on CC proliferation was assessed via an MTT assay. Results: MiR-484 was upregulated in most tumors, including CC, with DEGs enriched in skin development, PI3K signaling, and immune processes. High miR-484 expression correlated with specific immune cell infiltration, hypoxia, and drug sensitivity. Prognostic genes identified were predominantly epidermal and stratified patients with CC into risk groups, with the low-risk group showing enhanced survival and immunotherapeutic responses. qRT-PCR confirmed FGFR3 upregulation in CC cells, and an miR-484 mimic reversed the P. longifolia inhibitory effect on HeLa proliferation. Conclusion: MiR-484 plays a crucial role in the CC progression and prognosis, suggesting its potential as a biomarker for targeted therapy.

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Section: Discussionmentioning

confidence: 59%

Section: Mir-484 Expression Correlated With Tumor Stemness Hypoxia An...mentioning

confidence: 99%

Exploring MiR-484 Regulation by Polyalthia longifolia: A Promising Biomarker and Therapeutic Target in Cervical Cancer through Integrated Bioinformatics and an In Vitro Analysis

Niu,

Chen,

Chai

et al. 2024

Biomedicines

View full text Add to dashboard Cite

show abstract

TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma

Cited by 1 publication

References 26 publications

Exploring MiR-484 Regulation by Polyalthia longifolia: A Promising Biomarker and Therapeutic Target in Cervical Cancer through Integrated Bioinformatics and an In Vitro Analysis

Exploring MiR-484 Regulation by Polyalthia longifolia: A Promising Biomarker and Therapeutic Target in Cervical Cancer through Integrated Bioinformatics and an In Vitro Analysis

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