Triiodothyronine Stimulates Growth of Peripheral Blood Mononuclear Cells in Serum-Free Cultures in Uremic Patients

Blaehr, Hanne; Bregengaard, Claus; Povlsen, Johan Vestergaard

doi:10.1159/000168437

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…In vitro, T 4 substantially potentiates the ability of splenocytes to proliferate and to produce more interleukin 6 (IL-6) in the presence of concanavalin A (Con A) mitogen (Attia, 2001). T 3 also enhances phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) stimulation of human lymphocytes (Bloehr et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Recovery of age‐dependent immunological deterioration in old mice by thyroxine treatment

El-Shaikh

Gabry

Othman

2006

Animal Physiology Nutrition

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On the basis that multiple interactions exist between thyroid hormones and immune system, and ageing is accompanied by changes in thyroid hormone secretion, it seems possible that thyroid hormones may be involved in the age-related immune dysfunction. The present study was conducted to evaluate in vivo and in vitro effects of thyroxine (T(4)) treatment on both cell-mediated and humoral immune responses of aged mice. In a trial to improve age-associated immune dysfunction, T(4) (0.2, 1.0 and 5.0 microg) was subcutaneously supplemented to BALB/c mice (over 18 months old) for 30 consecutive days. The present results showed that exogenous treatment of aged mice with T(4) was associated with a marked increase in serum T(4) level, and the total number of peripheral blood leukocytes as well as the total cellularity of thymus, spleen, peripheral lymph nodes (PLNs), mesenteric lymph nodes (MLNs) and bone marrow (BM). T(4) treatment also caused a significant increase in the total and differential numbers of peritoneal exudate cells (PECs), while it caused a slight increase in macrophages' phagocytic activity of PEC. Moreover, T(4) treatment elicited a statistically significant increase in both plaque-forming cell and rosette-forming cell responses. In vitro results showed that the addition of T(4) at concentrations of 0.001, 0.005 and 0.025 microg/well substantially potentiated the ability of splenocytes from aged mice to proliferate in the presence of concanavalin-A mitogen. Histological examination of thymuses from T(4)-treated aged mice revealed that the cortex was preferentially enlarged and repopulated with immature thymocytes. The present study postulates that thyroid hormones may be involved in the observed decrease in the immune responsiveness during ageing, and that T(4) treatment to aged mice is able to restore the age-related decline of the immune efficiency.

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Section: Introductionmentioning

confidence: 99%

Recovery of age‐dependent immunological deterioration in old mice by thyroxine treatment

El-Shaikh

Gabry

Othman

2006

Animal Physiology Nutrition

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Serum‐free media for cultures of primitive and mature hematopoietic cells

Sandstrom

Miller

Papoutsakis

1994

Biotech & Bioengineering

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The in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum-containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum-free media. In this review, we summarize and discuss the current status of serum-free media for both mature and immature human hematopoietic cells. The mature hematopoietic cells discussed are of lymphoid (e.g., lymphokine activated killer cells and tumor infiltrating lymphocytes) and myeloid origin (e.g., monocytes/macrophages). The cultures of immature hematopoietic cells discussed are clonogenic and long-term cultures. In addition, we briefly review the types of human hematopoietic cells, their clinical applications, and the basic strategies and components used to formulate serum-free media, Finally, we outline future requirements and directions in the development of serum-free media for primitive hematopoietic cells.

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Impaired endotoxin-induced interleukin-1β secretion, not total production, of mononuclear cells from ESRD patients

Lonnemann

Barndt

Kaever

et al. 1995

Kidney International

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Lipopolysaccharide (LPS)-induced interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) production and secretion from peripheral blood mononuclear cells (PBMC) were determined in a longitudinal study with repeated measurements in PBMC from patients with chronic uremia not on hemodialysis (N = 8), end-stage renal disease (ESRD) patients (N = 8), and healthy controls (N = 7). ESRD patients were studied while using low-flux Cuprophan dialyzers and again using high-flux AN 69 dialyzers. Total (cell-associated plus secreted) LPS-induced IL-1 beta production was enhanced in uremic patients, but similar to controls in ESRD patients on Cuprophan. In contrast, LPS-induced IL-1 beta secretion (secreted amounts in % of total production) was similar to controls in uremic patients, but significantly reduced in ESRD patients on Cuprophan (P < 0.01). During AN 69 hemodialysis, LPS-induced total IL-1 beta production remained unchanged but IL-1 beta secretion increased significantly (P < 0.05) compared to Cuprophan dialysis. Increased IL-1 beta secretion coincided with a suppression in PGE2 synthesis (P < 0.02). Similarly, blockade of endogenous PGE2 by indomethacin increased LPS-induced IL-1 beta secretion (P < 0.01) but did not enhance total IL-1 beta production in PBMC from controls and patients on Cuprophan hemodialysis. Neither total production nor secretion of TNF alpha was different comparing the three study groups. We conclude that LPS-induced IL-1 beta secretion, but not total production, is impaired in PBMC from ESRD patients on long-term Cuprophan hemodialysis. This functional change in the PBMC response is specific for IL-1 beta, not due to uremia per se but hemodialysis-dependent and reversible. Hemodialysis with AN 69 suppresses endogenous PGE2 synthesis in PBMC which is associated with increased LPS-induced IL-1 beta secretion in the presence of unchanged total IL-1 beta production. We speculate that PGE2 could inactivate the IL-1 beta converting enzyme which is essential for processing and secretion of mature IL-1 beta.

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Triiodothyronine Stimulates Growth of Peripheral Blood Mononuclear Cells in Serum-Free Cultures in Uremic Patients

Cited by 3 publications

References 5 publications

Recovery of age‐dependent immunological deterioration in old mice by thyroxine treatment

Recovery of age‐dependent immunological deterioration in old mice by thyroxine treatment

Serum‐free media for cultures of primitive and mature hematopoietic cells

Impaired endotoxin-induced interleukin-1β secretion, not total production, of mononuclear cells from ESRD patients

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