Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells

Erzurumlu, Yalçın; Muhammed, Muhammed Tilahun

doi:10.1016/j.cellsig.2023.110745

Cited by 4 publications

(3 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have drawn attention to the regulation of ER quality control mechanism members and their effects on cancer progression in prostate cancer. [6,18,[28][29][30][31][32] ERQC (ER Quality Control Compartment) system is regulated by dynamic protein interactions involving many protein families. ERAD is one of the most effective cellular systems responsible for protein…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have drawn attention to the regulation of ER quality control mechanism members and their effects on cancer progression in prostate cancer. [ 6,18,28–32 ]…”

Section: Discussionmentioning

confidence: 99%

“…[6] Furthermore, other hormones, such as progesterone and thyroid hormone, have been shown to be capable of stimulating AR signaling in prostate cancer cells by acting like androgens. [29,30] In this context, it is very crucial to examine the possible regulation pattern of estrogen receptors in prostate cancer cells. Present data indicated that curcumin treatment strongly reduced the expression level of Erα protein in a dose-dependent manner for both prostate cancer cells, whereas it was not affected the Erβ levels (Figure 4).…”

Section: F I G U R Ementioning

confidence: 99%

See 2 more Smart Citations

New mode of action of curcumin on prostate cancer cells: Modulation of endoplasmic reticulum‐associated degradation mechanism and estrogenic signaling

Erzurumlu,

Dogan,

Catakli

2024

J Biochem & Molecular Tox

Self Cite

View full text Add to dashboard Cite

Prostate cancer is leading to cancer‐related mortality in numerous men each year worldwide. While there are several treatment options, acquired drug resistance mostly limits the success of treatments. Therefore, there is a need for the development of innovative treatments. Curcumin is one of the bioactive polyphenolic ingredients identified in turmeric and has numerous biological activities, such as anti‐inflammatory and anticancer. In the present study, we investigated the effect of curcumin on the ER‐associated degradation (ERAD) and estrogenic signaling in prostate cancer cells. The antiproliferative effect of curcumin on human androgen‐dependent prostate cancer cell lines LNCaP and VCaP was estimated by WST‐1 assay. Morphological alterations were investigated with an inverted microscope. We investigated the effect of curcumin on ERAD and estrogen signaling proteins by immunoblotting assay. To evaluate the impact of curcumin on endoplasmic reticulum (ER) protein quality‐related, the expression level of 32 genes was analyzed by quantitative reverse transcription polymerase chain reaction. The nuclear translocation of estrogen receptor was examined by nuclear fractionation and immunofluorescence microscopy. We found that curcumin effectively reduced the proliferation rates of LNCaP and VCaP cells. ERAD proteins; Hrd1, gp78, p97/VCP, Ufd1 and Npl4 were strongly induced by curcumin. Also, the steady‐state level of polyubiquitin was increased in a dose‐dependent manner in both cell lines. Curcumin administration remarkably decreased the protein levels of estrogen receptor‐alfa (Erα), whereas estrogen receptor‐beta unaffected. Additionally, curcumin strongly restricted the nuclear translocation of Erα. Present data suggest that curcumin may be effectively used in therapeutic approaches associated with the targeting ER protein quality control mechanism and modulation of estrogen signaling in prostate cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Recent studies have drawn attention to the regulation of ER quality control mechanism members and their effects on cancer progression in prostate cancer. [ 6,18,28–32 ]…”

Section: Discussionmentioning

confidence: 99%