Triglycerides analysis in adipose tissue from insulin sensitive and insulin resistance obese patients

Al-Sulaiti, Haya

doi:10.1016/j.atherosclerosis.2017.06.864

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“…The study of blood TG levels is of great interest in the study of obesity because their level quickly responds to dietary changes [ 133 , 134 ] and is associated with insulin resistance [ 135 , 136 ], lipotoxicity [ 137 ] and dyslipidaemia [ 138 ]. Despite this, studies of the profile of individual FAs in plasma TG are quite rare; this parameter is usually tested in adipose tissue, where TG accumulates [ 71 , 139 ], or in breast milk [ 140 ]. Usually, the isolated plasma TAG fraction is examined as one of the components in the study of the whole lipid profile [ 74 , 128 , 141 ].…”

Section: Preparation Of Different Sample Typesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.

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