Triggering receptor expressed on myeloid cells (TREM)‐1 participates in <i>Schistosoma mansoni</i> inflammatory responses

Cheng, Po Ching; Lin, Ching Nan; Chen, Y.-J.; Chang, Fumin; Tsaihong, John Chin; Lee, K.-M.

doi:10.1111/j.1365-3024.2011.01284.x

Cited by 14 publications

(9 citation statements)

References 43 publications

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“…TREM-1 is a DAP12-associated receptor expressed on neutrophils and monocytes ( 57 ) that plays a key role in the protecting the host against fungal allergens ( 58 ), parasites ( 59 ) as well as in bacterial sepsis ( 60 , 61 ), collagen-induced arthritis ( 62 ), and inflammation-associated tumor development ( 63 ). Interesting studies have also suggested that TREM-1 is involved in neutrophil migration across the epithelium ( 64 ) and macrophage infiltration at pathological sites ( 63 ) thus providing a possible explanation for the amplified inflammatory response we observed in our system.…”

Section: Discussionmentioning

confidence: 99%

Repetitive Exposure of IL-17 Into the Murine Air Pouch Favors the Recruitment of Inflammatory Monocytes and the Release of IL-16 and TREM-1 in the Inflammatory Fluids

et al. 2018

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The infiltration of Th17 cells in tissues and organs during the development of many autoimmune diseases is considered a key step toward the establishment of chronic inflammation. Indeed, the localized and prolonged release of IL-17 in specific tissues has been associated with an increased severity of the inflammatory response that remains sustained over time. The cellular and molecular mechanisms behind these effects are far from being clear. In this study we investigated the effects of two repetitive administration of recombinant IL-17 into the murine air pouch to simulate a scenario where IL-17 is released over time in a pre-inflamed tissue. Consistent with our previous observations, mice receiving a single dose of IL-17 showed a transitory influx of neutrophils into the air pouch that peaked at 24 h and declined at 48 h. Conversely, mice receiving a double dose of the cytokine—one at time 0 and the second after 24 h—showed a more dramatic inflammatory response with almost 2-fold increase in the number of infiltrated leukocytes and significant higher levels of TNF-α and IL-6 in the inflammatory fluids. Further analysis of the exacerbated inflammatory response of double-injected IL-17 mice showed a unique cellular and biochemical profile with inflammatory monocytes as the second main population emigrating to the pouch and IL-16 and TREM-1 as the most upregulated cytokines found in the inflammatory fluids. Most interestingly, mice receiving a double injection of IL-1β did not show any change in the cellular or biochemical inflammatory response compared to those receiving a single injection or just vehicle. Collectively these results shed some light on the function of IL-17 as pro-inflammatory cytokine and provide possible novel ways to target therapeutically the pathogenic effects of IL-17 in autoimmune conditions.

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Section: Discussionmentioning

confidence: 99%

Repetitive Exposure of IL-17 Into the Murine Air Pouch Favors the Recruitment of Inflammatory Monocytes and the Release of IL-16 and TREM-1 in the Inflammatory Fluids

et al. 2018

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“…Regarding Schistosoma infection, some substances from Schistosoma eggs may inhibit the expression of TREM-1 on mouse macrophages. This mechanism can decrease the macrophage-mediated inflammatory response in infected hosts [28]. On the other hand, neutrophils infected by L. infantum enhance the transcription of TREM-1 and molecules associated with its activation as well as the release of sTREM-1.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have explored the role of TREM-1 in inducing inflammation in multiple contexts, such as sepsis [9,21], cancer [22], viral infections [23,24], and others non-infectious diseases [25,26]. However, the role of TREM-1 signaling in innate immunity against parasite infections and its underlying mechanisms remain unknown [13,27,28].…”

Section: Discussionmentioning

confidence: 99%

Keratinocytes and Activation of TREM-1 Pathway in Cutaneous Leishmaniasis Lesions

Nunes

Ampuero

Bonyek-Silva

et al. 2021

Microbiology Research

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Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) amplifies the immune response, operating synergistically with Toll-Like Receptors (TLRs) in the production of inflammatory mediators. TREM-1 signaling depends on the adapter protein DAP12, which results in the activation of NFkB, the expression of inflammatory genes, and the release of antimicrobial peptides, such as Beta-defensin 2. We evaluated the activation of the TREM-1 signaling pathways in Cutaneous Leishmaniasis (CL) caused by Leishmania braziliensis and linage human keratinocytes exposed to these parasites since the host immune response against Leishmania plays a critical role in promoting parasite killing but also participates in inflammation and tissue damage. We analyzed publicly available transcriptome data from the lesions of CL patients. In the CL biopsies, we found increased expression of the molecules involved in the TREM-1 pathway. We then validated these findings with RT-qPCR and immunohistochemistry in newly obtained biopsies. Surprisingly, we found a strong labeling of TREM-1 in keratinocytes, prompting the hypothesis that increased TREM-1 activation may be the result of tissue damage. However, increased TREM-1 expression was only seen in human lineage keratinocytes following parasite stimulation. Moreover, no up-regulation of TREM-1 expression was observed in the skin lesions caused by other non-infectious inflammatory diseases. Together, these findings indicate that L. braziliensis (Lb) induces the expression of the TREM-1 receptor in tissue keratinocytes regardless of tissue damage, suggesting that non-immune skin cells may play a role in the inflammatory response of CL.

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“…The method was modified as our previously reports described [ 35 ]. The spleens of sacrificed mice were removed and placed in a Petri dish and then washed to flush out the spleen immune cells.…”

Section: Methodsmentioning

confidence: 99%

Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum

Cheng¹,

Lin²,

Peng³

et al. 2016

PLoS Negl Trop Dis

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Schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. Development of a vaccine is thought to be the most effective way to control this disease. Recombinant 26-kDa glutathione S-transferase (rSjGST) has previously been reported to achieve a worm reduction rate of 42–44%. To improve the efficiency of the vaccine against Schistosoma japonicum, we immunized mice with a combination of pcDNA vector-encoded 26-kDa SjGST (pcDNA/SjGST), IL-12 expressing-plasmid (pIL-12), and rSjGST. Co-vaccination with pcDNA/SjGST, pIL-12, and rSjGST led to a reduction in worm burden, hepatic egg burden, and the size of liver tissue granulomas than that in the untreated infection controls. In addition, we detected high levels of specific IgG, IgG1, and IgG2a against the rSjGST antigen in infected mice vaccinated with this combination of pcDNA/SjGST, pIL-12, and rSjGST. Moreover, high expression levels of Th2 cytokines, including IL-4 and IL-10, were also detected in this group, without diminished levels of IL-12, INF-γ, and TNF-α cytokines that are related to parasite killing. In conclusion, we have developed a new vaccination regimen against S. japonicum infection and shown that co-immunization with pcDNA/SjGST vaccine, pIL-12, and rSjGST has significant anti-parasite, anti-hepatic egg and anti-pathology effects in mice. The efficacy of this vaccination method should be further validated in large animals such as water buffalo. This method may help to reduce the transmission of zoonotic schistosomiasis japonica.

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Triggering receptor expressed on myeloid cells (TREM)‐1 participates in Schistosoma mansoni inflammatory responses

Cited by 14 publications

References 43 publications

Repetitive Exposure of IL-17 Into the Murine Air Pouch Favors the Recruitment of Inflammatory Monocytes and the Release of IL-16 and TREM-1 in the Inflammatory Fluids

Repetitive Exposure of IL-17 Into the Murine Air Pouch Favors the Recruitment of Inflammatory Monocytes and the Release of IL-16 and TREM-1 in the Inflammatory Fluids

Keratinocytes and Activation of TREM-1 Pathway in Cutaneous Leishmaniasis Lesions

Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum

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