Triggering of the macrophage and neutrophil respiratory burst by antibody bound to a spin-label phospholipid hapten in model lipid bilayer membranes

Dg, Hafeman; Jt, Lewis; Hm, McConnell

doi:10.1021/bi00564a037

Cited by 40 publications

(20 citation statements)

References 31 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7. The results are quite similar to those reported previously for a mouse macrophage cell line (3). DISCUSSION The present work was motivated by a desire to use a chemically and physically well-defined planar model membrane surface that serves as a target for specific cell surface recognition.…”

Section: Resultssupporting

confidence: 86%

“…The stimulation of macrophage [1-14C]glucose oxidation by haptenated lipid vesicles and antihapten antibodies was measured as described (3). Glucose oxidation by macrophages bound to the haptenated phospholipid monolayer was similarly determined except that the lipid monolayer on an 18-mm circular cover glass was placed in a 10-ml beaker (never reexposing the monolayer to any gas phase).…”

Section: -mentioning

confidence: 99%

“…Such reconstituted lipid bilayer membranes are usually in the form of multilamellar liposomes or single-shell vesicles. In some studies the question has arisen as to whether the spherical shape (e.g., radius of curvature 0.5 um), membrane flexibility, or both, of these vesicles or liposomes might play a significant role when cell membrane-reconstituted target membrane contact is involved in binding and triggering (2)(3)(4). For this reason we have been motivated to study the specific binding and triggering of cellular components of the immune system by lipid monolayer membranes attached to a planar glass surface.…”

mentioning

confidence: 99%

“…For this reason we have been motivated to study the specific binding and triggering of cellular components of the immune system by lipid monolayer membranes attached to a planar glass surface. In the present work, the specific antibody-dependent interactions of macrophages with the supported membranes have been studied, and the results have been compared with previous studies ofsimilar interactions of macrophages with vesicle membranes (2)(3)(4).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Specific antibody-dependent interactions between macrophages and lipid haptens in planar lipid monolayers.

Hafeman¹,

Tscharner²,

McConnell³

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The binding of -guinea pig peritoneal macrophages to planar lipid monolayers on alkylated glass is shown to be highly specific, requiring lipid hapten in the monolayer and specific anti-hapten IgG antibody. This is true for both "fluid" and "solid" monolayer membranes, in which the lateral diffusion coefficients of fluorescent lipid probes and bound fluorescent antibodies differ by at least two orders of magnitude. The region of the (macrophage membrane)-(monolayer membrane) contact is readily observed by using an epifluorescence microscope and fluoresceinated IgG antibodies or fluorescent lipids. The fluorescence intensity of IgG antibodies bound to lipid haptens in fluid monolayer membranes in the region of the (macrophage membrane)-(monolayer membrane) contact was significantly enhanced in the early stages of binding (first 10 min at 24TC), due to a diffusive flux of the fluorescent antibodies into the region of membrane-membrane contact. Cellular activation takes place immediately during a 10-min warm-up to 37C and can be recognized by rapid symmetrical cell spreading, the formation of"black holes" around the cells (probably due to superoxide-facilitated photochemical bleaching of the fluorophore), and the release of the-lysozomal enzyme cathepsin B. Specific antibody-dependent [1-"C]-glucose oxidation by these macrophages on fluid and solid monolayers is quite similar to that reported previously for fluid and solid bilayer vesicle target membranes. These results are significant for understanding the molecular interactions between membranes that are necessary for a macrophage cytotoxic response.Reconstituted lipid bilayer membranes containing specific antigens or lipid haptens have been used successfully in a number of studies of cell surface recognition by cellular and humoral components ofthe immune system (for examples ofrecent work and references to earlier literature, see ref. 1). Such reconstituted lipid bilayer membranes are usually in the form of multilamellar liposomes or single-shell vesicles. In some studies the question has arisen as to whether the spherical shape (e.g., radius of curvature 0.5 um), membrane flexibility, or both, of these vesicles or liposomes might play a significant role when cell membrane-reconstituted target membrane contact is involved in binding and triggering (2-4). For this reason we have been motivated to study the specific binding and triggering of cellular components of the immune system by lipid monolayer membranes attached to a planar glass surface. In the present work, the specific antibody-dependent interactions of macrophages with the supported membranes have been studied, and the results have been compared with previous studies ofsimilar interactions of macrophages with vesicle membranes (2-4).A second motivation for the present work has been a desire to use the-fluorescence microscope to visualize molecular events that take place at the interface between a cellular membrane and a reconstituted membrane during the course of a specific functional interact...

show abstract

Section: Resultssupporting

confidence: 86%

Section: -mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Specific antibody-dependent interactions between macrophages and lipid haptens in planar lipid monolayers.

Hafeman¹,

Tscharner²,

McConnell³

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…If we extrapolate from studies of the number of hapten-antibody-receptor bonds necessary for specific antibody-dependent binding and phagocytosis of lipid vesicles by RAW264 macrophages (with which fewer than 100 such bonds are sufficient for binding and phagocytosis in a strongly stirred system), it is plausible that 60 G-H-2Kk pairs would suffice for both binding and triggering of T-cell responses (33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.

Cartwright¹,

Smith²,

Heinzelmann³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

It is shown that liposomes containing (i) a fluorescein-labeled murine histocompatibility antigen and the G protein of vesicular stomatitis virus or (ii) H-2Kk and fluorescein-labeled viral protein (FITC-G) can elicit H-2-restricted syngeneic antiviral cytotoxic T 'cells as assayed by 5tCr release from appropriate virus-infected target cells. Fluorescence recovery after photobleaching was -used to measure the diffusion coefficients of these reconstituted proteins in four different sam-ples: (i) FITC-H-2Kk; jiU) FITC-H-2Kk and G; (iii) FITC-G; and (iv) FITC-G and H-2K . The same rate of lateral diffusion (D' = 1 X 10-8 cm2/sec at 37C in 25% cholesterol/75% dimyristoylphosphatidylcholine) was obtained in every case. Both proteins, fluorescent as well as nonfluorescent, could be patched by using specific antibodies. When G was patched with antibody, FITC-H2Kk did not copatch. When H-2Kk was patched with antibody, FITC-G did not copatch. These diffusion and patching measurements rule out the possibility that these proteins have either extensive oligomeric associations or strong specific pairwise associations.There is now much evidence that virus-specific cytotoxic T lymphocytes (CTL) are dually specific for.virus and for selfcell surface antigens encoded by the major histocompatibility complex (1). Effector lymphocytes sensitized against virus-infected cells of a given haplotype will lyse virus-infected cells of the same haplotype with much higher efficiency than virus-infected cells of a different haplotype. A similar restriction holds for the afferent immune response. Virus-specific H-2-restricted secondary elicitation of CTL has been demonstrated by using membrane fragments as well as reconstituted membranes (2-11). These studies show that both viral protein and H-2 antigen must be present in the same reconstituted membrane in order to elicit the cellular response.Three models can be considered, based on alternative hypothetical structures for the T-cell receptor(s). In one model, a specific molecular complex between viral protein and transplantation antigen preexists in the target membrane before interaction with the T cell and is recognized by the T-cell receptor. In the second model, a close physical association of the two antigens is stabilized only during their interaction with the Tcell receptor. In the third model, no close physical or chemical association between viral protein and transplantation antigen exists prior to, or during, interaction with the T cell. (This is the two-receptor or "dual receptor" model.) The T-cell receptor(s) involved in the secondary elicitation of CTL is presumed to be on precytotoxic T cells or on T helper cells or on both.One biophysical approach to this problem is to use fluorescently labeled membrane components so that the distribution, motion, and interaction of these components can be studied by using optical techniques. In the work described here, purified G protein (G) of vesicular stomatitis virus (VSV) and purified H-2Kk protein were fluorescently labeled an...

show abstract

Liposomes as Carriers of Vaccines

Alving¹

1989

Progress in Vaccinology

View full text Add to dashboard Cite

Triggering of the macrophage and neutrophil respiratory burst by antibody bound to a spin-label phospholipid hapten in model lipid bilayer membranes

Cited by 40 publications

References 31 publications

Specific antibody-dependent interactions between macrophages and lipid haptens in planar lipid monolayers.

Specific antibody-dependent interactions between macrophages and lipid haptens in planar lipid monolayers.

H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.

Liposomes as Carriers of Vaccines

Contact Info

Product

Resources

About