Triggering of Suicidal Erythrocyte Death by Amphotericin B

Mahmud, Hasan; Mauro, Daniele; Qadri, Syed M.; Föller, Michael; Läng, Florian

doi:10.1159/000233251

Cited by 45 publications

(25 citation statements)

References 75 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier studies have indeed shown that excessive eryptosis contributes to the anemia of several disorders, such as iron deficiency [20], phosphate depletion [31], Hemolytic Uremic Syndrome [32], sepsis [33], malaria [14,[34][35][36][37][38][39], or Wilson's disease [40]. Furthermore, several anemia producing xenobiotics and endogeneous substances are at least partially effective through stimulation of eryptosis, such as cordycepin [41], amyloid peptides [42], lipopeptides [43], retinoic acid [44], amantadine [45], thymol [46], ciglitazone [47], amphotericin B [48], valinomycin [21], listeriolysin [49], copper [38], bismuth [50], tin [51], cadmium [52], selenium [53], vanadate [18], gold [54] and arsenic [55].…”

Section: Discussionmentioning

confidence: 99%

Monensin Induced Suicidal Erythrocyte Death

Bhavsar

Eberhard

Bobbala

et al. 2010

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Eryptosis, the suicidal erythrocyte death, is characterized by cell membrane scrambling and cell shrinkage. Eryptosis may be triggered by excessive hyperosmotic or isosmotic cell shrinkage leading to increase of cytosolic Ca²⁺ concentration. Eryptosis is further stimulated by the K⁺ ionophore valinomycin, which leads to exit of KCl and osmotically obliged water, or by energy (glucose) depletion, which compromises the function of the Na⁺/K⁺ ATPase thus increasing cytosolic Na⁺ concentration. The present study explored whether the Na⁺ ionophore monensin affects erythrocyte cell volume and eryptosis. The cell membrane scrambling was estimated from binding of annexin V to phosphatidylserine at the erythrocyte surface, cell volume from forward scatter in FACS analysis, cytosolic Ca²⁺ concentration from Fluo3 fluorescence and the cytosolic ATP concentration from a luciferase-based assay. Within 24 hours, exposure to monensin (0.1-10 µg/ml) significantly increased forward scatter, cytosolic Ca²⁺ concentration and annexin V-binding. Glucose depletion was followed by decreased forward scatter and increased cytosolic Ca²⁺ concentration and annexin V-binding. The effect on forward scatter was partially reversed, the effect on cytosolic Ca²⁺ concentration and annexin V binding augmented by additional treatment with monensin. In conclusion, monensin dissociates the alterations of cell membrane and cell volume in suicidal erythrocyte death.

show abstract

Section: Discussionmentioning

confidence: 99%

Monensin Induced Suicidal Erythrocyte Death

Bhavsar

Eberhard

Bobbala

et al. 2010

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…The model drug AmB and its commercial formulations are well known for hemotoxicity, due to eryptosis triggered by cell membrane scrambling and cell shrinkage through the stimulation of increased Ca 2ϩ entry into erythrocytes (42). AmB-induced hemolysis was studied in vitro, providing a reliable measure for estimating the membrane damage caused in vivo.…”

Section: Figmentioning

confidence: 99%

Immunoadjuvant Chemotherapy of Visceral Leishmaniasis in Hamsters Using Amphotericin B-Encapsulated Nanoemulsion Template-Based Chitosan Nanocapsules

Asthana

Jaiswal

Gupta

et al. 2013

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The accessible treatment options for life-threatening neglected visceral leishmaniasis (VL) disease have problems with efficacy, stability, adverse effects, and cost, making treatment a complex issue. Here we formulated nanometric amphotericin B (AmB)-encapsulated chitosan nanocapsules (CNC-AmB) using a polymer deposition technique mediated by nanoemulsion template fabrication. CNC-AmB exhibited good steric stability in vitro, where the chitosan content was found to be efficient at preventing destabilization in the presence of protein and Ca

show abstract

“…Ceramide stimulates the death of a wide variety of cells including neurons [67][68][69], Tlymphocytes [70], hepatocytes [71], epithelial cells [72] and erythrocytes [30,42,44,71,73]. Sphingolipids participate in cancer [64,[74][75][76][77][78][79], inflammatory disease [52,56,80,81] including lung inflammation and infection [82], cystic fibrosis [83], cardiovascular disease [84][85][86], Wilson´s disease [71], diabetes [87][88][89][90] and several neurological diseases [17,18,67,[91][92][93][94].…”

Section: Discussionmentioning

confidence: 99%

Sphingosine but not Sphingosine-1-phosphate Stimulates Suicidal Erythrocyte Death

Qadri

Bauer

Zelenak

et al. 2011

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Sphingosine kinase 1 phosphorylates sphingosine, which is converted to ceramide by ceramide synthetase. Ceramide triggers eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure at the erythrocyte surface. Erythrocytes lack sphingosine phosphate-degrading enzymes and thus store large quantities of sphingosine phosphate. The present study explored the influence of sphingosine and sphingosine phosphate on eryptosis. [Ca²⁺]_i, was estimated from Fluo3 fluorescence, cell volume from forward scatter and PS exposure from annexin V-binding in FACS analysis. Sphingosine (0.1 – 10 µM) but not sphingosine-1- phosphate (0.1 – 10 µM) increased [Ca²⁺]_i, decreased cell volume and increased PS-exposure. The observations disclose sphingosine, but not sphingosine-1-phosphate, as a strong inducer of eryptosis.

show abstract

Triggering of Suicidal Erythrocyte Death by Amphotericin B

Cited by 45 publications

References 75 publications

Monensin Induced Suicidal Erythrocyte Death

Monensin Induced Suicidal Erythrocyte Death

Immunoadjuvant Chemotherapy of Visceral Leishmaniasis in Hamsters Using Amphotericin B-Encapsulated Nanoemulsion Template-Based Chitosan Nanocapsules

Sphingosine but not Sphingosine-1-phosphate Stimulates Suicidal Erythrocyte Death

Contact Info

Product

Resources

About