Trifolium species-derived substances and extracts—Biological activity and prospects for medicinal applications

Kołodziejczyk-Czepas, Joanna

doi:10.1016/j.jep.2012.06.048

Cited by 88 publications

(83 citation statements)

References 72 publications

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“…The majority of studies on the biological properties of Trifolium pratense L. (Fabaceae) (red clover) are focused on its phytoestrogenic action, being a result of isoflavone content (Kolodziejczyk-Czepas, 2012). These phytoestrogens can inhibit angiogenesis and red clover extract might be a powerful chemopreventive agent (Kolodziejczyk-Czepas, 2012;Krenn & Paper, 2009). Dietary isoflavones derived from red clover may also halt the progression of prostate cancer by inducing apoptosis in low to moderate-grade tumors with only minimal adverse effects (Jarred et al, 2002;Kolodziejczyk-Czepas, 2012).…”

Section: Herbsmentioning

confidence: 99%

“…These phytoestrogens can inhibit angiogenesis and red clover extract might be a powerful chemopreventive agent (Kolodziejczyk-Czepas, 2012;Krenn & Paper, 2009). Dietary isoflavones derived from red clover may also halt the progression of prostate cancer by inducing apoptosis in low to moderate-grade tumors with only minimal adverse effects (Jarred et al, 2002;Kolodziejczyk-Czepas, 2012). Extract of the other herb from Papilionaceae family, Lotus corniculatus L. (Fabaceae) (bird's-foot trefoil) may also be a potential antitumor agent inhibiting the viability of macrophage-like murine tumor cells (Reza et al, 2012).…”

Section: Herbsmentioning

confidence: 99%

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Estonian folk traditional experiences on natural anticancer remedies: From past to the future

Sak¹,

Jürisoo

Raal

2014

Pharmaceutical Biology

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Context: Despite diagnostic and therapeutic advancements, the burden of cancer is still increasing worldwide. Toxicity of current chemotherapeutics to normal cells and their resistance to tumor cells highlights the urgent need for new drugs with minimal adverse side effects. The use of natural anticancer agents has entered into the area of cancer research and increased efforts are being made to isolate bioactive products from medicinal plants. Objective: To lead the search for plants with potential cytotoxic activity, ethnopharmacological knowledge can give a great contribution. Therefore, the attention of this review is devoted to the natural remedies traditionally used for the cancer treatment by Estonian people over a period of almost 150 years. Methods: Two massive databases, the first one stored in the Estonian Folklore Archives and the second one in the electronic database HERBA (http://herba.folklore.ee/), containing altogether more than 30 000 ethnomedicinal texts were systematically reviewed to compile data about the Estonian folk traditional experiences on natural anticancer remedies.

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Section: Herbsmentioning

confidence: 99%

Section: Herbsmentioning

confidence: 99%

Estonian folk traditional experiences on natural anticancer remedies: From past to the future

Sak¹,

Jürisoo

Raal

2014

Pharmaceutical Biology

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“…Recently, some clovers have been also recognized as a source of substances with the therapeutic effects that may be utilized in modern medicine. Most of the available literature contains reports on physiological and medicinal properties of red clover (T. pratense) (Kolodziejczyk-Czepas, 2012;Sabudak & Guler, 2009). The ethnopharmacological recommendations of T. pratense include the treatment of sore throat, fever, pneumonia and meningitis, lung illnesses, skin problems, as well as some disorders of nervous and reproductive systems (KolodziejczykCzepas, 2012).…”

Section: Introductionmentioning

confidence: 99%

Free radical scavenging actions of threeTrifoliumspecies in the protection of blood plasma antioxidant capacityin vitro

Kołodziejczyk-Czepas

Nowak

Moniuszko-Szajwaj

et al. 2015

Pharmaceutical Biology

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Context: Three clover [Trifolium L. (Leguminosae)] species were selected on the basis of data from traditional medicine, phytochemical profiles, and agricultural significance. Objective: The in vitro evaluations of free radical scavenging properties, ferric reducing abilities, and antioxidant effects of extracts from T. pratense L. (crude extract and phenolic fraction), T. pallidum L., and T. scabrum L. (phenolic fractions) were performed. Materials and methods: Activities of the Trifolium extracts were determined at their final concentrations of 1.5-50 mg/ml. Free radical scavenging properties of methanol extract solutions were estimated by the reduction of DPPH and ABTS radicals. Measurements of the total antioxidant capacity (TAC) were carried out to assess the antioxidant activities of the extracts in human blood plasma under conditions of oxidative stress, induced by 200 mM peroxynitrite. Results: The phenolic fraction of T. pratense displayed the strongest ABTS and DPPH radical scavenging effects (EC 50 value of 21.69 and 12.27 mg/ml, respectively). The EC 50 value for T. pallidum extract attained 29.77 and 30.06 mg/ml. The two remaining extracts were less potent scavengers (EC 50 value higher than 50 mg/ml). Similar differences were obtained during evaluation of the ferric reducing abilities. Analysis of antioxidant properties of the extracts in blood plasma did not provide such evident differences in their actions, however, it indicated that the T. pratense phenolic fraction displayed the strongest effect. Conclusions: The examined Trifolium extracts partly protected blood plasma and enhanced its non-enzymatic antioxidant defense against harmful action of peroxynitrite in vitro.

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“…Previous studies reveal that clover contains high levels of isoflavones (Kagan and Flythe, 2012;Kaurinovic et al, 2012;Kolodziejczyk-Czepas, 2012;Kicel and Wolbiś, 2013). Clover isoflavones are the most studied phytoestrogens after soy isoflavones, and are considered to be healthy because of their biological and pharmacological uses (Sabudak and Guler, 2009).…”

Section: Introductionmentioning

confidence: 97%

Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense

Hh¹,

Cq²,

R³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and then screened under cephalosporin. IFS expression was detected by reverse transcriptionpolymerase chain reaction. The IFS gene was cloned successfully. Sequence analysis indicated that IFS gene had high homology with similar genes from other plants. The IFS-overexpressing callus was obtained by introducing the LBA4404-harboring IFS-pRI101-AN-IFS vector into T. pratense calluses.

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Trifolium species-derived substances and extracts—Biological activity and prospects for medicinal applications

Cited by 88 publications

References 72 publications

Estonian folk traditional experiences on natural anticancer remedies: From past to the future

Estonian folk traditional experiences on natural anticancer remedies: From past to the future

Free radical scavenging actions of threeTrifoliumspecies in the protection of blood plasma antioxidant capacityin vitro

Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense

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