Trifolium pratense: Friable calli, cell culture protocol and isoflavones content in wild plants, in vitro and cell cultures analyzed by UPLC

Reis, Ângelo Vieira dos; Scopel, Marina; Zuanazzi, José Ângelo Silveira

doi:10.1016/j.bjp.2018.06.004

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…g −1 DW AR Jiang et al ( 2017 ) Isoflavones Trifolium pratense 23.53 mg . g −1 DW IVP Reis et al ( 2018 ) Isoquercetin Ocimum basilicum 3.72 mg . g −1 DW S Açıkgöz ( 2020 ) Rosmarinic acid Ocimum basilicum 22.53 mg .…”

Section: Production Via Plant In Vitro Culturesmentioning

confidence: 99%

“…g −1 DW flavonoids (rutin, neohesperidin, buddleoside, liquiritigenin, quercetin, isorhamnetin, kaempferol, and isoliquiritigenin)(Gai et al 2019 ). The formation of isoflavones in the in vitro cultivated whole plants of Trifolium pratense was investigated by Reis et al ( 2018 ). After a cultivation period of 60 days in vitro plants showed a higher total isoflavone content (23.55 mg .…”

Section: Flavonoids and Phenolic Acidsmentioning

confidence: 99%

“…Chlorogenic AR adventitious root culture, DW dry weight, HR hairy roots, IVP in vitro cultivated plant, S cell suspension culture, SH shoot culture (Gai et al 2019). The formation of isoflavones in the in vitro cultivated whole plants of Trifolium pratense was investigated by Reis et al (2018). After a cultivation period of 60 days in vitro plants showed a higher total isoflavone content (23.55 mg .…”

Section: Flavonoids and Phenolic Acidsmentioning

confidence: 99%

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Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Wawrosch

Zotchev

2021

Appl Microbiol Biotechnol

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Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cultivated raw material. The biotechnological manufacturing of such compounds offers a number of advantages like predictable, stable, and year-round sustainable production, scalability, and easier extraction and purification. Plant cell and tissue culture represents one possible alternative to the extraction of phytochemicals from plant material. Although a broad commercialization of such processes has not yet occurred, ongoing research indicates that plant in vitro systems such as cell suspension cultures, organ cultures, and transgenic hairy roots hold a promising potential as sources for bioactive compounds. Progress in the areas of biosynthetic pathway elucidation and genetic manipulation has expanded the possibilities to utilize plant metabolic engineering and heterologous production in microorganisms. This review aims to summarize recent advances in the in vitro production of high-value plant secondary metabolites of medicinal importance.Key points• Bioactive plant secondary metabolites are important for current and future use in medicine• In vitro production is a sustainable alternative to extraction from plants or costly chemical synthesis• Current research addresses plant cell and tissue culture, metabolic engineering, and heterologous production Graphical abstract

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Section: Production Via Plant In Vitro Culturesmentioning

confidence: 99%

Section: Flavonoids and Phenolic Acidsmentioning

confidence: 99%

Section: Flavonoids and Phenolic Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Wawrosch

Zotchev

2021

Appl Microbiol Biotechnol

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“…For example Negrel and Javelle, (1995), reported that this could be caused by defense responses produced by enzymes acting on the cell wall, initiating the biosynthesis of various metabolites even in the same cell lines. It is argued that the production of certain compounds in cell cultures may not occur because their origin is undifferentiated cells, and as a consequence these cells lack specific metabolites and therefore require tissue-specific biosynthesis (Reis et al, 2018).…”

Section: Evaluating Of Bioactive Metabolitesmentioning

confidence: 99%

Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

Özsan

Onus

2022

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] has many health-protecting properties due to its valuable bioactive components which are not stable and produced in high amounts in the raw plant material in nature. These bioactive components that gaining interest can be increased thanks to the contribution of valuable in vitro techniques, such as callus and cell suspension cultures, and various new applications such as elicitor treatments. The present study aimed to determined bioactive components in three globe artichoke cultivars by using callus cell suspension cultures in various media combinations and by applying two elicitor treatments, namely methyl jasmonate and chitosan, at 3 different concentrations (methyl jasmonate 50 µM, 100 µM, and 200 µM; chitosan 200 mg L -1 , 400 mg L -1 , and 800 mg L -1 ) with 3 different application durations (24h, 48h, and 72h). The bioactive compounds profile of cultivars was determined by HPLC-DAD. Obtained results revealed that using wellbalanced concentrations of auxin: cytokinin (1:1 or 10:1) in a media composition is a must for triggering the callus formation process for globe artichoke. Results also showed that accumulated bioactive components and their amounts varied based on cultivars. Experiment results revealed that different types of elicitors other than methyl jasmonate and chitosan, or different doses of elicitors and application durations should be used/tested to get desired levels of bioactive components. The findings of the present study may play a supportively and complementarily mission in several important fields such as agriculture, and pharmaceutical engineering.

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“…This raw material source makes it possible to obtain secondary metabolites in higher quantities. Cultures do not require large maintenance costs and in addition, the extraction process is facilitated, since the cells in cultures are not as complex as in full-fledged plants [9].…”

Section: Introductionmentioning

confidence: 99%

The effect of biohanin A isolated from the callus culture of meadow clover (Trifolium pratense L.) on the life expectancy and survival of Caenorhabditis elegans

Milentyeva

Drozdova

2022

Preprint

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Oxidative stress, in which healthy aging is limited, is a problem, since in the process there is an uncontrolled production of reactive radicals that negatively affect cells. Heat stress is closely related to oxidative stress, as it stimulates the production of radicals. Meadow clover (Trifolium pratense L.) is a promising source of isoflavonoids that have various positive effects on the body. The aim of the work is to study the effect of biohanin A, isolated from Trifolium pratense L. callus cultures, on the life expectancy of the C. elegans model organism, as well as its survival under oxidative and thermal stress.It was found that biohanin A increased the lifespan of C. elegans worms. The best concentration was determined - 100 microns, at which the survival rate of nematodes after 61 days of incubation was 16.0%. After 48 hours, the survival rate of worms was the highest (87.1%) at a concentration of biohanin A of 200 microns. Other concentrations 10, 50, 100 (81,2, 83,0, 82,4 %) they also showed a higher survival rate compared to the control (74.0%). In addition, the survival rate under heat stress of C. elegans was higher by 12.0; 8.4; 4.0% compared with the control. Thus, the results show the antioxidant potential of biohanin A from plant material under oxidative and thermal stress. The substance also had a positive effect on the lifespan of C. elegans.

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Trifolium pratense: Friable calli, cell culture protocol and isoflavones content in wild plants, in vitro and cell cultures analyzed by UPLC

Cited by 7 publications

References 40 publications

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

The effect of biohanin A isolated from the callus culture of meadow clover (Trifolium pratense L.) on the life expectancy and survival of Caenorhabditis elegans

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