Triethyleneglycol dimethacrylate addition improves the 3D-printability and construct properties of a GelMA-nHA composite system towards tissue engineering applications

Comeau, Patricia; Willett, Thomas L.

doi:10.1016/j.msec.2020.110937

Cited by 21 publications

(12 citation statements)

References 39 publications

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“…In this case, high geometric accuracy would result in a printability index of P r = 1 (square shape transversal pore geometry), while P r < 1 and P r > 1 correspond to a more round or irregular shaped transversal geometry, respectively. 42 , 46 , 170 , 187 − 189 Thus, the measurement of pore circularity and the deviation from an ideal square, can provide an estimation of the shape fidelity in the x – y plane. 46 Low viscosity and nonoptimal gelation conditions are some of the underlying causes for these deviations, e.g., the merging of filaments and the resulting low shape fidelity.…”

Section: Assessment Of Printability and Shape Fidelitymentioning

confidence: 99%

Printability and Shape Fidelity of Bioinks in 3D Bioprinting

et al. 2020

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Three-dimensional bioprinting uses additive manufacturing techniques for the automated fabrication of hierarchically organized living constructs. The building blocks are often hydrogel-based bioinks, which need to be printed into structures with high shape fidelity to the intended computer-aided design. For optimal cell performance, relatively soft and printable inks are preferred, although these undergo significant deformation during the printing process, which may impair shape fidelity. While the concept of good or poor printability seems rather intuitive, its quantitative definition lacks consensus and depends on multiple rheological and chemical parameters of the ink. This review discusses qualitative and quantitative methodologies to evaluate printability of bioinks for extrusion- and lithography-based bioprinting. The physicochemical parameters influencing shape fidelity are discussed, together with their importance in establishing new models, predictive tools and printing methods that are deemed instrumental for the design of next-generation bioinks, and for reproducible comparison of their structural performance.

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Section: Assessment Of Printability and Shape Fidelitymentioning

confidence: 99%

Printability and Shape Fidelity of Bioinks in 3D Bioprinting

et al. 2020

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“…Over the last few decades, light-curable polymers have become an attractive strategy employed in the construction of hydrogels for tissue engineering applications [ 20 , 21 ]. Methacrylic anhydride (Ma) has been used to modify gelatin by substituting methacryloyl substitution groups onto the reactive amine so as to obtain photoreactive gelatin methacrylate (GelMa), which can be crosslinked when exposed to UV light [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Shie

Lee

et al. 2020

Polymers

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Gelatin-methacryloyl (GelMa) is a very versatile biomaterial widely used in various biomedical applications. The addition of methacryloyl makes it possible to have hydrogels with varying mechanical properties due to its photocuring characteristics. In addition, gelatin is obtained and derived from natural material; thus, it retains various cell-friendly motifs, such as arginine-glycine-aspartic acid, which then provides implanted cells with a friendly environment for proliferation and differentiation. In this study, we fabricated human dermal fibroblast cell (hDF)-laden photocurable GelMa hydrogels with varying physical properties (5%, 10%, and 15%) and assessed them for cellular responses and behavior, including cell spreading, proliferation, and the degree of extracellular matrix remodeling. Under similar photocuring conditions, lower concentrations of GelMa hydrogels had lower mechanical properties than higher concentrations. Furthermore, other properties, such as swelling and degradation, were compared in this study. In addition, our findings revealed that there were increased remodeling and proliferation markers in the 5% GelMa group, which had lower mechanical properties. However, it was important to note that cellular viabilities were not affected by the stiffness of the hydrogels. With this result in mind, we attempted to fabricate 5–15% GelMa scaffolds (20 × 20 × 3 mm3) to assess their feasibility for use in skin regeneration applications. The results showed that both 10% and 15% GelMa scaffolds could be fabricated easily at room temperature by adjusting several parameters, such as printing speed and extrusion pressure. However, since the sol-gel temperature of 5% GelMa was noted to be lower than its counterparts, 5% GelMa scaffolds had to be printed at low temperatures. In conclusion, GelMa once again was shown to be an ideal biomaterial for various tissue engineering applications due to its versatile mechanical and biological properties. This study showed the feasibility of GelMa in skin tissue engineering and its potential as an alternative for skin transplants.

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“…The struts and the pores were more well-defined in the CS10 group as compared to the CS0 group. The ability of the bioink to extrude as a consistent filament, the structure of the first layer, rheology behavior, and cross-linking capabilities are all properties that significantly influence bioink printability [32]. In addition, the printability of CS/GelMa bioinks through straight needles was impressed by the adding of CS powder in this study, as shown by the 3D-printed 6-layer scaffold.…”

Section: Synthesis and Characterization Of The Cs/gelma Scaffoldmentioning

confidence: 73%

Bidirectional Differentiation of Human-Derived Stem Cells Induced by Biomimetic Calcium Silicate-Reinforced Gelatin Methacrylate Bioink for Odontogenic Regeneration

et al. 2021

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Tooth loss or damage is a common problem affecting millions of people worldwide, and it results in significant impacts on one’s quality of life. Dental regeneration with the support of stem cell-containing scaffolds has emerged as an alternative treatment strategy for such cases. With this concept in mind, we developed various concentrations of calcium silicate (CS) in a gelatin methacryloyl (GelMa) matrix and fabricated human dental pulp stem cells (hDPSCs)-laden scaffolds via the use of a bioprinting technology in order to determine their feasibility in promoting odontogenesis. The X-ray diffraction and Fourier transform-infrared spectroscopy showed that the incorporation of CS increased the number of covalent bonds in the GelMa hydrogels. In addition, rheological analyses were conducted for the different concentrations of hydrogels to evaluate their sol–gel transition temperature. It was shown that incorporation of CS improved the printability and printing quality of the scaffolds. The printed CS-containing scaffolds were able to release silicate (Si) ions, which subsequently significantly enhanced the activation of signaling-related markers such as ERK and significantly improved the expression of odontogenic-related markers such as alkaline phosphatase (ALP), dentin matrix protein-1 (DMP-1), and osteocalcin (OC). The calcium deposition assays were also significantly enhanced in the CS-containing scaffold. Our results demonstrated that CS/GelMa scaffolds were not only enhanced in terms of their physicochemical behaviors but the odontogenesis of the hDPSCs was also promoted as compared to GelMa scaffolds. These results demonstrated that CS/GelMa scaffolds can serve as cell-laden materials for future clinical applications and use in dentin regeneration.

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Triethyleneglycol dimethacrylate addition improves the 3D-printability and construct properties of a GelMA-nHA composite system towards tissue engineering applications

Cited by 21 publications

References 39 publications

Printability and Shape Fidelity of Bioinks in 3D Bioprinting

Printability and Shape Fidelity of Bioinks in 3D Bioprinting

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Bidirectional Differentiation of Human-Derived Stem Cells Induced by Biomimetic Calcium Silicate-Reinforced Gelatin Methacrylate Bioink for Odontogenic Regeneration

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