Trienoic Fatty Acids and Plant Tolerance of High Temperature

Murakami, Yuuki; Tsuyama, Michito; Kobayashi, Yoshitaka; Kodama, Hiroaki; Iba, Koh

doi:10.1126/science.287.5452.476

Cited by 390 publications

(285 citation statements)

References 19 publications

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“…Mutants with smaller decreases in thylakoid unsaturation are substantially indistinguishable from wild type when grown at 22°C, but exhibit defects in biogenesis and maintenance of chloroplasts at temperatures below 5°C. These mutants include fad5, fad6, the double mutant fad7 fad8, and the triple mutant fad3 fad7 fad8 (Hugly and Somerville, 1992;Murakami et al, 2000;Routaboul et al, 2000). A role for thylakoid unsaturation in maintaining photosynthetic function at low temperatures is also supported by experiments in which transgenic expression of fatty acid desaturases in chillingsensitive plant species resulted in increased survival of plants at low temperatures (Kodama et al, 1994;Ishizaki-Nishizawa et al, 1996;Murakami et al, 2000).…”

mentioning

confidence: 66%

“…The Arabidopsis desaturation mutants fad5, fad6, fad7 fad8, and fad3 fad7 fad8 all show chlorosis and reduced growth rates when grown at 4°C, but the plants are nevertheless able to complete their life cycle (Hugly and Somerville, 1992;Murakami et al, 2000;Routaboul et al, 2000). Another Arabidopsis mutant, fatty acid biosynthesis 1 (fab1), exhibits a distinct lowtemperature phenotype.…”

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confidence: 99%

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A Suppressor of fab1 Challenges Hypotheses on the Role of Thylakoid Unsaturation in Photosynthetic Function

Barkan

Vijayan²,

Carlsson³

et al. 2006

Plant Physiology

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Leaf membrane lipids of the Arabidopsis (Arabidopsis thaliana) fatty acid biosynthesis 1 (fab1) mutant contain a 35% to 40% increase in the predominant saturated fatty acid 16:0, relative to wild type. This increase in membrane saturation is associated with loss of photosynthetic function and death of mutant plants at low temperatures. We have initiated a suppressor screen for mutations that allow survival of fab1 plants at 2°C. Five suppressor mutants identified in this screen all rescued the collapse of photosynthetic function observed in fab1 plants. While fab1 plants died after 5 to 7 weeks at 2°C, the suppressors remained viable after 16 weeks in the cold, as judged by their ability to resume growth following a return to 22°C and to subsequently produce viable seed. Three of the suppressors had changes in leaf fatty acid composition when compared to fab1, indicating that one mechanism of suppression may involve compensating changes in thylakoid lipid composition. Surprisingly, the suppressor phenotype in one line, S31, was associated with a further substantial increase in lipid saturation. The overall leaf fatty acid composition of S31 plants contained 31% 16:0 compared with 23% in fab1 and 17% in wild type. Biochemical and genetic analysis showed that S31 plants contain a new allele of fatty acid desaturation 5 (fad5), fad5-2, and are therefore partially deficient in activity of the chloroplast 16:0 D7 desaturase. A double mutant produced by crossing fab1 to the original fad5-1 allele also remained alive at 2°C, indicating that the fad5-2 mutation is the suppressor in the S31 (fab1 fad5-2) line. Based on the biophysical characteristics of saturated and unsaturated fatty acids, the increased 16:0 in fab1 fad5-2 plants would be expected to exacerbate, rather than ameliorate, low-temperature damage. We propose instead that a change in shape of the major thylakoid lipid, monogalactosyldiacylglycerol, mediated by the fad5-2 mutation, may compensate for changes in lipid structure resulting from the original fab1 mutation. Our identification of mutants that suppress the low-temperature phenotype of fab1 provides new tools to understand the relationship between thylakoid lipid structure and photosynthetic function.

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confidence: 66%

mentioning

confidence: 99%

A Suppressor of fab1 Challenges Hypotheses on the Role of Thylakoid Unsaturation in Photosynthetic Function

Barkan

Vijayan²,

Carlsson³

et al. 2006

Plant Physiology

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“…1B) suggest that ROS may be generated by heat stress, which can initiate the peroxidation and destruction of lipids with subsequent membrane damage [26]. In support of this hypothesis, it has also been determined that heat stress induced oxidative stress in plants, which caused lipid peroxidation and membrane injury [27].…”

Section: Thermostability Of Cell Membranes Under Heat Stressmentioning

confidence: 90%

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

et al. 2007

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The present study investigated rice leaf proteome in response to heat stress. Rice seedlings were subjected to a temperature of 427C and samples were collected 12 and 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress frequently was generated in rice leaves exposed to high temperature. 2-DE, coupled with MS, was used to investigate and identify heat-responsive proteins in rice leaves. In order to identify the low-abundant proteins in leaves, samples were prefractionated by 15% PEG. The PEG supernatant and the pellet fraction samples were separated by 2-DE, and visualized by silver or CBB staining. Approximately 1000 protein spots were reproducibly detected on each gel, wherein 73 protein spots were differentially expressed at least at one time point. Of these differentially expressed proteins, a total of 34 and 39 protein spots were found in the PEG supernatant and pellet fractions, respectively. Using MALDI-TOF MS, a total of 48 proteins were identified. These proteins were categorized into classes related to heat shock proteins, energy and metabolism, redox homeostasis, and regulatory proteins. The results of the present study show that a group of low molecular small heat shock proteins (sHSPs) were newly induced by heat stress. Among these sHSPs, a low molecular weight mitochondrial (Mt) sHSP was validated further by Western blot analysis. Furthermore, four differentially accumulated proteins that correspond to antioxidant enzymes were analyzed at the mRNA level, which confirmed the differential gene expression levels, and revealed that transcription levels were not completely concomitant with translation. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants.

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“…A considerable body of work was centered on the metabolic step of fatty acid desaturation in response to temperature fluctuations, namely, the activities of various fatty acid desaturases (Murata et al, 1992;Murata and Los, 1997;Murakami et al, 2000;Iba, 2002;Wallis and Browse, 2002). However, the link between fatty acid desaturases and modulation of desaturation under temperature stress remains a weak one.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Biochemical Basis of Temperature-Induced Lipid Pathway Adjustments in Plants

et al. 2015

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Glycerolipid biosynthesis in plants proceeds through two major pathways compartmentalized in the chloroplast and the endoplasmic reticulum (ER). The involvement of glycerolipid pathway interactions in modulating membrane desaturation under temperature stress has been suggested but not fully explored. We profiled glycerolipid changes as well as transcript dynamics under suboptimal temperature conditions in three plant species that are distinctively different in the mode of lipid pathway interactions. In Arabidopsis thaliana, a 16:3 plant, the chloroplast pathway is upregulated in response to low temperature, whereas high temperature promotes the eukaryotic pathway. Operating under a similar mechanistic framework, Atriplex lentiformis at high temperature drastically increases the contribution of the eukaryotic pathway and correspondingly suppresses the prokaryotic pathway, resulting in the switch of lipid profile from 16:3 to 18:3. In wheat (Triticum aestivum), an 18:3 plant, low temperature also influences the channeling of glycerolipids from the ER to chloroplast. Evidence of differential trafficking of diacylglycerol moieties from the ER to chloroplast was uncovered in three plant species as another layer of metabolic adaptation under temperature stress. We propose a model that highlights the predominance and prevalence of lipid pathway interactions in temperature-induced lipid compositional changes.

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Trienoic Fatty Acids and Plant Tolerance of High Temperature

Cited by 390 publications

References 19 publications

A Suppressor of fab1 Challenges Hypotheses on the Role of Thylakoid Unsaturation in Photosynthetic Function

A Suppressor of fab1 Challenges Hypotheses on the Role of Thylakoid Unsaturation in Photosynthetic Function

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

Understanding the Biochemical Basis of Temperature-Induced Lipid Pathway Adjustments in Plants

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