Tricycle Project – One Health approach: Whole genome sequencing(WGS) of Extended-spectrum beta-lactamase (ESBL) producing Eschericia (E.) coli derived from human, food chain and environment

Hashim, Rahmat; Husin, Salbiah; Ahmad, Noraini; Bahari, Nur Syafiqah Kamarul; Abu, Noor Hidayah; Ali, Rehman; Hashim, Norazlina; Ramasamy, P.; Saberi, Marzieh; Laine, L. Yee; Rashid, Faisal; Rasid, N.A. Abd; Paul, Sayantan

doi:10.1016/j.ijid.2021.12.249

Cited by 10 publications

(8 citation statements)

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“…The bio-surveillance system based on monitoring the frequency rates of ESBL-positive bacteria, especially E. coli , has become a primary indicator for tracking AMR globally [ 19 ]. This system goes beyond a singular focus by embracing the One Health approach, which considers the interconnectedness of animal, human and environmental health in tackling AMR across these sectors [ 1 , 5 ]. Additionally, it facilitates direct comparisons of AMR prevalence and patterns across various regions worldwide.…”

Section: Discussionmentioning

confidence: 99%

“…β-lactamases include a wide variety of hydrolytic enzymes that tend to destabilize the structural integrity of β-lactam antibiotics through degradation of the amide bond of the β-lactam ring [ 4 ]. ESBL-producing bacteria are used as bio-surveillance markers to map the emergence and extent of antimicrobial resistance (AMR) under one-health research paradigms [ 5 ]. Classically, the ESBL-producing bacteria species were thought to be associated with clinical settings which would transfer mainly via nosocomial, and community-acquired channels [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Saeed,

Khan,

Ehtisham-ul-Haque

et al. 2023

Antibiotics

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Extended-spectrum β-lactamases (ESBL) give rise to resistance against penicillin and cephalosporin antibiotics in multiple bacterial species. The present study was conducted to map genetic determinants and related attributes of ESBL-producing bacteria in three wild aquatic bird species and chickens at the “Trimmu Barrage” in district Jhang, Punjab province, Pakistan. To study the prevalence of ESBL-producing bacteria, a total of 280 representative samples were collected from wild bird species; cattle egrets (Bubulcus ibis), little egrets (Egretta garzetta) and common teals (Anas crecca) as well as from indigenous chickens (Gallus gallus domesticus) originating from a local wet market. The isolates were confirmed as ESBL producers using a double disc synergy test (DDST) and bacterial species were identified using API-20E and 20NE strips. A polymerase chain reaction (PCR) was used to detect ESBL genetic determinants and for genus identification via 16S rRNA gene amplification. A phenotypic antimicrobial susceptibility test was performed for ESBL-producing isolates against 12 clinically relevant antibiotics using the Kirby–Bauer disk diffusion susceptibility test. A phylogenetic tree was constructed for the sequence data obtained in this study and comparative sequence data obtained from GenBank. The overall prevalence of ESBL-producing bacteria was 34.64% (97/280). The highest percentage (44.28%; 31/70) of ESBL-producing bacteria was recovered from chickens (Gallus gallus domesticus), followed by little egrets (Egretta garzetta) (41.43%; 29/70), common teal (Anas crecca) (28.57%; 20/70) and cattle egrets (Bubulcus ibis) (24.28%; 17/70). Five different ESBL-producing bacteria were identified biochemically and confirmed via 16S rRNA gene sequencing, which included Escherichia coli (72; 74.23%), Enterobacter cloacae (11; 11.34%), Klebsiella pneumoniae (8; 8.25%), Salmonella enterica (4; 4.12%) and Pseudomonas aeruginosa (2; 2.06%). Based on PCR, the frequency of obtained ESBL genes in 97 isolates was blaCTX-M (51.55%), blaTEM (20.62%), blaOXA (6.18%) and blaSHV (2.06%). In addition, gene combinations blaCTX-M + blaTEM, blaTEM + blaOXA and blaCTX-M + blaSHV were also detected in 16.49%, 2.06% and 1.03% of isolates, respectively. The ESBL gene variation was significant (p = 0.02) in different bacterial species while non-significant in relation to different bird species (p = 0.85). Phylogenetic analysis of amino acid sequence data confirmed the existence of CTX-M-15 and TEM betalactamases. The average susceptibility of the antibiotics panel used was lowest for both Klebsiella pneumoniae (62.5% ± 24.42) and Salmonella enterica (62.5% ± 31.08) as compared to Enterobacter cloacae (65.90% ± 21.62), Pseudomonas aeruginosa (70.83% ± 33.42) and Escherichia coli (73.83% ± 26.19). This study provides insight into the role of aquatic wild birds as reservoirs of ESBL-producing bacteria at Trimmu Barrage, Punjab, Pakistan. Hence, active bio-surveillance and environment preservation actions are necessitated to curb antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Saeed,

Khan,

Ehtisham-ul-Haque

et al. 2023

Antibiotics

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“…Due to its antibacterial properties, basil oil has the potential to be developed as a source of active ingredients for antibacterial products [5]. Pathogenic bacteria such as Staphylococcus aureus, which is a gram-positive bacteria, and Escherichia coli, a gram-negative, usually attack humans [6]. Therefore, an antibacterial activity test is conducted to determine the various kinds of antibacterial activities contained in basil oil.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Basil Leaves Essential Oil using Microwave Assisted Hydrodistillation Method: Physical Characterization and Antibacterial Activity

Putri,

Nugraha

et al. 2023

CHEESA

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Basil oil can be obtained from basil leaves by non-extraction methods, namely Microwave Assisted Hydrodistillation (MAHD). Therefore, this research aims to determine the yield percentage, essential oil composition by GC-MS, physical characteristics, and antibacterial activity of basil essential oil. The highest yield of 0.3076% was obtained at the optimum condition, which included a microwave power of 300 W, a mass-to-volume solvent ratio (F/S) of 0.75 g/mL, a raw material size of ± 1.75 cm, and an extraction time of 90 min. The results of the analysis of variance showed that all process parameters used had a significant effect on the yield obtained. Basil oil exhibited a larger inhibition zone against Escherichia coli bacteria (16.38 mm) which tended to be stronger than Staphylococcus aureus (5.95 mm) and was classified as moderate. The main components contained in the basil oil were E-Citral (46.79%) and Z-Citral (38.17%). The physical characteristic test showed that the basil oil was soluble in 96% ethanol after a ratio of 1:9, with 1 ml of basil oil compared to 9 ml of ethanol. The density of basil oil at 0.961 g/mL also complied with the standard value according to the Essential Oil Association (EOA) of Ocimum basilicum Essential Oil. These results showed revealed that the parameter analyzed using oil yields at operating conditions produced the most optimum yield value.

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“…ESBL-PE and CRE/CPE have been detected in Irish waters, including bathing waters, in recent years (Hooban et al, 2021(Hooban et al, , 2022Mahon et al, 2017Mahon et al, , 2019, with ESBL-PE and CPE detected in 78 % and 15 % of water samples tested respectively (Hooban et al, 2022). It has been proposed that monitoring for specific AMR organisms, such as EBSL-producing E. coli (Hashim et al, 2022;Matheu et al, 2017), should be included as a routine part of bathing water testing in the revision of the EU bathing water directive, which is currently ongoing (European Commission, 2022).…”

Section: Introductionmentioning

confidence: 99%

Assessing the Impact of Recreational Water Use on Carriage of Antimicrobial Resistant Organisms

Farrell

Chueiri

Connor

et al. 2023

Preprint

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Tricycle Project – One Health approach: Whole genome sequencing(WGS) of Extended-spectrum beta-lactamase (ESBL) producing Eschericia (E.) coli derived from human, food chain and environment

Cited by 10 publications

References 0 publications

Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Extraction of Basil Leaves Essential Oil using Microwave Assisted Hydrodistillation Method: Physical Characterization and Antibacterial Activity

Assessing the Impact of Recreational Water Use on Carriage of Antimicrobial Resistant Organisms

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