Triclosan at environmentally relevant concentrations promotes horizontal transfer of multidrug resistance genes within and across bacterial genera

Lu, Ji; Wang, Yue; Li, Jie; Mao, Likai; Nguyen, Son Hoang; Duarte, Tânia; Coin, Lachlan; Bond, Philip L.; Yuan, Zhiguo; Guo, Jianhua

doi:10.1016/j.envint.2018.10.040

Cited by 199 publications

(130 citation statements)

References 41 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…Therefore, these studies on animals or humans support the increase in ROS in the presence of these non-antibiotic pharmaceuticals. In addition to these non-antibiotic pharmaceuticals, biocides (e.g., triclosan) and heavy metals were also demonstrated to increase ROS generation levels, impose stress-response on bacteria, thus enhancing the uptake potential of conjugal plasmids 60–62 . Further studies are required to confirm if other non-antibiotic pharmaceuticals follow this pattern of enhancing intracellular ROS generation and potentially contributing to increased bacterial gene transfer.…”

Section: Discussionmentioning

confidence: 99%

“…Escherichia coli K-12 LE392 with RP4 plasmid (resistant to tetracycline, kanamycin and ampicillin) was the donor. Pseudomonas putida KT2440 with high resistance towards chloramphenicol was the recipient 20, 60 . Culture conditions are described in Text S1.…”

Section: Methodsmentioning

confidence: 99%

“…In order to analyze the gene expression levels during the conjugative process, the same conjugation experiments were performed as described above and RNA was extracted after 2-h’s mating with either 0.5 mg/L ibuprofen, naproxen, gemfibrozil, diclofenac, propanolol, or 0.1 mg/L iopromide. As bacterial mRNA expressions respond quickly to external stress, 2-h’s mating time was chosen as done previously 47, 60 . Total RNA (containing the mixture of donor and recipient bacteria) was extracted using RNeasy Mini Kit (QIAGEN ® , Germany) with an extra bead-beating step for the cell lysis process 20 .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Non-antibiotic pharmaceuticals can enhance the spread of antibiotic resistance via conjugation

Wang

Zhang

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

9Antibiotic resistance is a global threat for public health. It is widely acknowledged that 10 antibiotics at sub-inhibitory concentrations are important in disseminating antibiotic 11 resistance via horizontal gene transfer. While there is high use of non-antibiotic human-12 targeted pharmaceuticals in our societies, the potential contribution of these on the spread of 13 antibiotic resistance has been overlooked so far. Here, we report that commonly consumed 14 non-antibiotic pharmaceuticals, including nonsteroidal anti-inflammatories (ibuprofen, 15 naproxen, diclofenac), a lipid-lowering drug (gemfibrozil), and a β-blocker (propanolol), at 16 clinically and environmentally relevant concentrations, significantly accelerated the 17 conjugation of plasmid-borne antibiotic resistance genes. We looked at the response to these 18 drugs by the bacteria involved in the gene transfer through various analyses that included 19 monitoring reactive oxygen species (ROS) and cell membrane permeability by flow 20 cytometry, cell arrangement, and whole-genome RNA and protein sequencing. We found the 21 enhanced conjugation correlated well with increased production of ROS and cell membrane 22 permeability. We also detected closer cell-to-cell contact and upregulated conjugal genes. 23Additionally, these non-antibiotic pharmaceuticals caused the bacteria to have responses 24 similar to those detected when exposed to antibiotics, such as inducing the SOS response, and 25 enhancing efflux pumps. The findings advance our understanding of the bacterial transfer of 26 antibiotic resistance genes, and importantly emphasize concerns of non-antibiotic human-27 targeted pharmaceuticals for enhancing the spread of antibiotic resistance. 28 29 Key Words: antibiotic resistance; non-antibiotic pharmaceuticals; horizontal gene transfer; 30 conjugation; reactive oxygen species; cell membrane 31 approximately 95% of the drug market 9,10 , the role of non-antibiotic pharmaceuticals in the 51 emergence and spread of antibiotic resistance has received relatively little attention. Recently, 52Maier et al. 11 screened more than 1 000 marketed drugs against 40 representative gut 53 bacterial strains, and reported that more than 200 non-antibiotic pharmaceuticals could 54 exhibit antibiotic-like effects on the bacteria. They found these non-antibiotic 55 pharmaceuticals contributed to the emergence of antibiotic resistance through mutation or 56 increased expression of efflux pump genes 11 . However, they did not investigate if these non-57 4 antibiotic pharmaceuticals can facilitate HGT 11 . If non-antibiotic pharmaceuticals have 58 effects on the spread of antibiotic resistance, there may be features and properties of the non-59 antibiotic pharmaceuticals, or shared mechanisms, that promote the horizontal transfer of 60 ARGs. 61 62In this study, we investigated the potential of different types of commonly consumed non-63 antibiotic human-targeted pharmaceuticals for promoting conjugative transfer of plasmid 64 borne ARGs. We also explored the un...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Non-antibiotic pharmaceuticals can enhance the spread of antibiotic resistance via conjugation

Wang

Zhang

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…There have been attempts to sequence bacterial plasmids using MinION device as part of a whole genome assembly [136,138] or in exclusively studies [139,164]. As mentioned earlier, studying the whole structure of replicons is important as they are responsible for the horizontal gene transfer between strains, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…The ONT MinION benefits sequencing short genomes, such as viruses, in many ways especially in terms of genome assembly. In fact, we regularly obtain nanopore reads that cover the whole length of the circular IncP-α plasmid RP4 (around 60kpb long) genomes [164], which significantly assist in the assembly phase. Even without associated Illumina data, nanopore data can generate results with decent quality after consensus calling thanks to 5.1 Introduction 103 sufficient high coverage [50].…”

Section: Introductionmentioning

confidence: 99%

Real-time analysis for Nanopore sequencing data

Nguyen¹

View full text Add to dashboard Cite

strain and 15-fold coverage for the type strain. From the quality histograms, the red lines represent 2D reads' scores which are clearly better than the ones in 1D reads (green and blue for template and complement sequences respectively). The length distribution shows that MinION sequencing returned significant reads with length around 5K for the first sample and 7K for the latter. The statistics on the far right of the table show error rate in term of indels, mismatches and hit rate from alignments with reference data. Overall, chemistry R7.3 is more up-to-date than R7 and as expected, gave slightly better results. As a next step, there came a mission to assembly those reads, together with available Illumina data of corresponding samples, in an attempt to have complete genome reconstructions for these two K. pneumoniae strains. This task was important at the time as it would depict bigger picture about interactions between genetic elements in the whole genome and help to comprehend the biological pathways supporting this superbug's mechanisms. 1.3.1 Definition Despite the huge improvements of sequencing technology, there still exist the common limitation that in most cases, it is impossible to sequence directly the whole length of a genome. As shown in Figure 1.5, the actual practice is to break the whole genome into smaller pieces 1.4 Genome assembly with long read data Nanopore data can be used on its own for de novo genome assembly, or in conjunction with high quality SGS data in a process called hybrid assembly. Both approaches have their own pros and cons and should be considered in reference to available dataset and the ultimate purposes of using the final assembly. This is unlike SGS sequencing-by-synthesis platforms, which sequence billions of short reads in parallel, with each cycle (contributing an extra base) taking several minutes, with the data being provided in batch at the end of a run. For this task, the raw data of a read must be retrieved and analyzed while sequencing is

show abstract

A review on triclosan in wastewater: Mechanism of action, resistance phenomenon, environmental risks, and sustainable removal techniques

Jabłońska‐Trypuć

2023

Water Environment Research

View full text Add to dashboard Cite

Triclosan, belonging to the bisphenols, is known antiseptic broad‐spectrum biocide. It has a very wide range of applications, both in health care, as well as in the household. Triclosan enters the environment, both water bodies and soil, because of its high prevalence and the ability to accumulation. Excessive use of antimicrobial formulations may cause the generation of resistance among microorganisms. Reduced susceptibility to triclosan is observed more frequently and in an expanded group of microorganisms, and is conditioned by a number of different mechanisms occurring on the molecular level. Conventional wastewater treatment processes are not always able to provide a reliable barrier to triclosan. Therefore, additional advanced treatment technologies are being considered in areas, where a triclosan contamination problem has been identified. Removal of triclosan from wastewater is carried out using different biological and chemical techniques, however it should be pointed out that physico‐chemical methods often generate toxic by‐products. Toxicity of triclosan and its degradation products, bacterial resistance to this compound and evident problems with triclosan elimination from wastewater are currently the main problems faced by companies creating products containing triclosan.

show abstract

Triclosan at environmentally relevant concentrations promotes horizontal transfer of multidrug resistance genes within and across bacterial genera

Cited by 199 publications

References 41 publications

Non-antibiotic pharmaceuticals can enhance the spread of antibiotic resistance via conjugation

Non-antibiotic pharmaceuticals can enhance the spread of antibiotic resistance via conjugation

Real-time analysis for Nanopore sequencing data

A review on triclosan in wastewater: Mechanism of action, resistance phenomenon, environmental risks, and sustainable removal techniques

Contact Info

Product

Resources

About