Triciribine attenuates pathological neovascularization and vascular permeability in a mouse model of proliferative retinopathy

Shan, Shengshuai; Liu, Fang; Ford, Edith; Caldwell, Ruth B.; Narayanan, S. Priya; Somanath, Payaningal R.

doi:10.1016/j.biopha.2023.114714

Cited by 5 publications

(2 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased vascular permeability in mice after treatment with LPS was studied by measuring the extravasation of albumin [40]. Mice were intracardially perfused with PBS (to clear blood from tissues) and then with 4% paraformaldehyde in PBS for fixing tissues.…”

Section: Measurement Of Vascular Permeability Using Albumin Immunosta...mentioning

confidence: 99%

See 1 more Smart Citation

Pharmacological Modulation of β-Catenin Preserves Endothelial Barrier Integrity and Mitigates Retinal Vascular Permeability and Inflammation

Rudraraju,

Shan,

Liu

et al. 2023

JCM

Self Cite

View full text Add to dashboard Cite

Compromised blood-retinal barrier (BRB) integrity is a significant factor in ocular diseases like uveitis and retinopathies, leading to pathological vascular permeability and retinal edema. Adherens and tight junction (AJ and TJ) dysregulation due to retinal inflammation plays a pivotal role in BRB disruption. We investigated the potential of ICG001, which inhibits β-catenin-mediated transcription, in stabilizing cell junctions and preventing BRB leakage. In vitro studies using human retinal endothelial cells (HRECs) showed that ICG001 treatment improved β-Catenin distribution within AJs post lipopolysaccharide (LPS) treatment and enhanced monolayer barrier resistance. The in vivo experiments involved a mouse model of LPS-induced ocular inflammation. LPS treatment resulted in increased albumin leakage from retinal vessels, elevated vascular endothelial growth factor (VEGF) and Plasmalemmal Vesicle-Associated Protein (PLVAP) expression, as well as microglia and macroglia activation. ICG001 treatment (i.p.) effectively mitigated albumin leakage, reduced VEGF and PLVAP expression, and reduced the number of activated microglia/macrophages. Furthermore, ICG001 treatment suppressed the surge in inflammatory cytokine synthesis induced by LPS. These findings highlight the potential of interventions targeting β-Catenin to enhance cell junction stability and improve compromised barrier integrity in various ocular inflammatory diseases, offering hope for better management and treatment options.

show abstract

Section: Measurement Of Vascular Permeability Using Albumin Immunosta...mentioning

confidence: 99%

“…The RNA isolation and analysis of retinal tissues were carried out as previously described [40]. Briefly, the retinal tissue was homogenized using a Micro-Tube homogenizer (Cat No.…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%