Trichostatin A (TSA) improves the development of rabbit‐rabbit intraspecies cloned embryos, but not rabbit‐human interspecies cloned embryos

Shi, Lihong; Miao, Yi‐Liang; Ouyang, Ying‐Chun; Huang, Jianqiang; Lei, Zili; Yang, Jianhua; Han, Zhiming; Song, Xie‐Yan; Sun, Qing‐Yuan; Chen, Da‐Yuan

doi:10.1002/dvdy.21450

Cited by 85 publications

(67 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Together, with hypermethylation of porcine fibroblast nuclei , this may be the reason for failure of nucleoli formation in porcine-rabbit iSCNT embryos. However, rabbit oocytes were shown to support preimplantation development of embryos derived from nuclei of several species, including bovine , Capra ibex (Jiang et al 2005), chicken , camel and Tibetan antelope (Zhao et al 2006), macaca (Yang et al 2003), cat and panda (Wen et al 2005), and even human (Shi et al 2008). However, microarray analysis failed to detect significant human genome reprogramming in human-rabbit iSCNT embryos (Chung et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

View full text Add to dashboard Cite

The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

show abstract

Section: Discussionmentioning

confidence: 99%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

View full text Add to dashboard Cite

show abstract

“…However, supplementation of reprogramming factors and even substitution of individual factors with small molecules has been demonstrated to drastically increase the efficiency of reprogramming. Components used include HDAC inhibitors such as valproic acid, suberoylanilide hydroxamic acid and trichostatin A, DNA methyltransferase inhibitors such as 5'-azacytidine (5'-azaC) and RG108, histone methyltransferase inhibitors such as BIX01294 and Ca-channel activators such as R(+)Bay K 8644 (Huangfu et al, 2008a;Shi et al, 2008). Addition of valproic acid, which is also beneficial in SCNT (Huangfu et al, 2008a), increased reprogramming efficiency 100-fold and allowed for successful reprogramming to occur without the addition of factors Klf4 and c-myc, two known oncogenes (Huangfu et al, 2008b).…”

Section: Methods For Generation Of Induced Pluripotent Stem Cellsmentioning

confidence: 99%

Natural and artificial routes to pluripotency

Krueger¹,

Swanson²,

Tanasijevic³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Pluripotent cells of the blastocyst inner cell mass (ICM) and their in vitro derivatives, embryonic stem (ES) cells, contain genomes in an epigenetic state that are poised for subsequent differentiation. Their chromatin is hyperdynamic in nature and relatively uncondensed. In addition, a large number of genes are expressed at low levels in both ICM and ES cells. Also, the chromatin of naturally pluripotent cells contains specialized histone modification patterns such as bivalent domains, which mark genes destined for later developmentally-regulated expression states. Female pluripotent cells contain X chromosomes that have yet to undergo the process of X chromosome inactivation. Collectively, these features of very early embyronic chromatin are required for the successful specification and production of differentiated cell lineages. Artificial reprogramming methods such as somatic nuclear transfer (SCNT), ES cell fusion-mediated reprogramming (FMR), and induced pluripotency (iPS) yield pluripotent cells that recapitulate many features of naturally pluripotent cells, including many of their epigenetic features. However, the route to pluripotent epigenomic states in artificial pluripotent cells differs drastically from that of their natural counterparts. Here, we compare and contrast the differing routes to pluripotency under natural and artificial conditions. In addition, we discuss the intrinsically metastable nature of the pluripotent epigenome and consider epigenetic aspects of reprogramming that may lead to incomplete or inaccurate reprogrammed states. Artificial methods of reprogramming hold immense promise for the development of autologous cell graft sources and for the development of cell culture models for human genetic disorders. However, the utility of artificially reprogrammed cells is highly dependent on the fidelity of the reprogramming process and it is therefore critically important to assess the epigenetic similarities between embryonic and induced pluripotent stem cells.

show abstract

“…On the other hand, many studies have reported production of iSCNT morulae and blastocysts (BL) also when nucleus donor cells and recipient oocytes had a very distant taxonomical relation, like in the case of inter-family bovine-pig (Dominko et al 1999, Uhm et al 2007, inter-order cat-and panda-rabbit (Wen et al 2005), camel-and Tibetan antelope-rabbit (Zhao et al 2006), human-rabbit (Shi et al 2008), dog-pig (Sugimura et al 2009), tiger-pig (Hashem et al 2007, human-bovine (Chang et al 2003, Illmensee et al 2006, Li et al 2008 or inter-classes chicken-rabbit combinations. However, this approach remains ineffective.…”

Section: Introductionmentioning

confidence: 99%

Development, embryonic genome activity and mitochondrial characteristics of bovine–pig inter-family nuclear transfer embryos

Lagutina¹,

Fulka²,

Brevini³

et al. 2010

Reproduction

View full text Add to dashboard Cite

The best results of inter-species somatic cell nuclear transfer (iSCNT) in mammals were obtained using closely related species that can hybridise naturally. However, in the last years, many reports describing blastocyst development following iSCNT between species with distant taxonomical relations (inter-classes, inter-order and inter-family) have been published. This indicates that embryonic genome activation (EGA) in xeno-cytoplasm is possible, albeit very rarely. Using a bovine-pig (inter-family) iSCNT model, we studied the basic characteristics of EGA: expression and activity of RNA polymerase II (RNA Pol II), formation of nucleoli (as an indicator of RNA polymerase I (RNA Pol I) activity), expression of the key pluripotency gene NANOG and alteration of mitochondrial mass. In control embryos (obtained by IVF or iSCNT), EGA was characterised by RNA Pol II accumulation and massive production of poly-adenylated transcripts (detected with oligo dT probes) in blastomere nuclei, and formation of nucleoli as a result of RNA Pol I activity. Conversely, iSCNT embryos were characterised by the absence of accumulation and low activity of RNA Pol II and inability to form active mature nucleoli. Moreover, in iSCNT embryos, NANOG was not expressed, and mitochondria mass was significantly lower than in intra-species embryos. Finally, the complete developmental block at the 16-25-cell stage for pig-bovine iSCNT embryos and at the four-cell stage for bovine-pig iSCNT embryos strongly suggests that EGA is not taking place in iSCNT embryos. Thus, our experiments clearly demonstrate poor nucleus-cytoplasm compatibility between these animal species.

show abstract

Trichostatin A (TSA) improves the development of rabbit‐rabbit intraspecies cloned embryos, but not rabbit‐human interspecies cloned embryos

Cited by 85 publications

References 49 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Natural and artificial routes to pluripotency

Development, embryonic genome activity and mitochondrial characteristics of bovine–pig inter-family nuclear transfer embryos

Contact Info

Product

Resources

About