Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases the<i>In Vitro</i>Developmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos

Samiec, M.; Opiela, Jolanta; Lipiński, Daniel; Romanek, Joanna

doi:10.1155/2015/814686

Cited by 38 publications

(39 citation statements)

References 70 publications

(100 reference statements)

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“…For example, 41.4% of SCNT embryos reached the blastocyst stage and had an average cell number of about 33 cells; of these, roughly 1–2 cells were TUNEL positive. These parameters are similar to previous observations of porcine SCNT blastocysts (Liang, Zhao, Choi, Kim, & Cui, ; Samiec, Opiela, Lipiński, & Romanek, ). During SCNT experiments for donor cell treatments, we noticed negative association between the length of time, or order, in which the embryos were produced, and development to the blastocyst stage, as well as with the amount of DNA degradation within those blastocysts.…”

Section: Discussionsupporting

confidence: 90%

Pharmacologic treatment of donor cells induced to have a Warburg effect‐like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs

Mordhorst

Murphy

Ross

et al. 2018

Molecular Reproduction Devel

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Somatic cell nuclear transfer is a valuable technique for the generation of genetically engineered animals, however, the efficiency of cloning in mammalian species is low (1-3%). Differentiated somatic cells commonly used in nuclear transfer utilize the tricarboxylic acid cycle and cellular respiration for energy production. Comparatively the metabolism of somatic cells contrasts that of the cells within the early embryos which predominately use glycolysis. Early embryos (prior to implantation) are evidenced to exhibit characteristics of a Warburg Effect (WE)-like metabolism. We hypothesized that pharmacologically driven fibroblast cells can become more blastomere-like and result in improved in vitro embryonic development after SCNT. The goals were to determine if subsequent in vitro embryo development is impacted by (1) cloning pharmacologically treated donor cells pushed to have a WE-like metabolism or (2) culturing non-treated donor clones with pharmaceuticals used to push a WE-like metabolism. Additionally, we investigated early gestational survival of the donor-treated clone embryos. Here we demonstrate that in vitro development of clones is not hindered by pharmacologically treating either the donor cells or the embryos themselves with CPI, PS48, or the combination of these drugs. Furthermore, these experiments demonstrate that early embryos (or at least in vitro produced embryos) have a low proportion of mitochondria which have high membrane potential and treatment with these pharmaceuticals does not further alter the mitochondrial function in early embryos. Lastly, we show that survival in early gestation was not different between clones from pharmacologically induced WE-like donor cells and controls.

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Section: Discussionsupporting

confidence: 90%

Pharmacologic treatment of donor cells induced to have a Warburg effect‐like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs

Mordhorst

Murphy

Ross

et al. 2018

Molecular Reproduction Devel

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“…Despite lack of study using this method in production of cattle NT embryos, the application of this method using AF-NT reconstructed oocytes resulted in 8.8% to 13.2% and 19.5% blastocyst rate in sheep (Hosseini et al, 2013a, Hosseini et al, 2013b and goat (Hosseini et al, 2015), respectively. Regarding the use of stem cells as donor cells, the porcine MSC-NT reconstructed oocytes had a higher developmental rate compared to the fetal fibroblast-NT derived counterparts , Samiec et al, 2015. The higher appropriate remodeling of stem cell-NT derived embryos has proved in different species (Bosch et al, 2006, Faast et al, 2006, Kumar et al, 2012, Lee et al, 2006, Rideout et al, 2000.…”

Section: Discussionmentioning

confidence: 99%

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari¹,

Shirazi²,

Shams‐Esfandabadi³

et al. 2016

Int. J. Dev. Biol.

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Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to express the genes which are a prerequisite for normal early embryo development. This study was aimed to evaluate the developmental competence and the expression pattern of some reprogramming related genes in bovine cloned embryos reconstructed with amniotic membrane stem cells (AMSCs) in comparison with those reconstructed with mesenchymal stem cells (MSCs) and adult fibroblasts (AF) as well as with in vitro fertilized (IVF) oocytes. In vitro matured abattoir-derived oocytes were considered as recipients and a hand-made cloning technique was employed for oocyte enucleation and nuclear transfer (NT) procedures. The expression pattern of genes involved in self-renewal and pluripotency (POU5F1, SOX2, NANOG), imprinting (IGF2, IGF2R), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), and apoptosis (BAX, BCL2) were evaluated in NT and IVF derived embryos. Despite the insignificant difference in cleavage rate between reconstructed and IVF oocytes, the blastocyst rate in the IVF group was higher than that of other groups. Among reconstructed oocytes, a higher blastocysts rate was observed in MSC-NT and AMSCs-NT derived embryos that were significantly higher than AF-NT derived ones. There were more similarities in the expression pattern of pluripotency and epigenetic modification genes between MSC-NT and IVF derived blastocysts compared with other groups. In conclusion, considering developmental competence, AMSCs, as alternative donors in SCNT procedure, like MSCs, were prone to have more advantage compared with AF.

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“…Histone deacetylatase inhibitors (HDACi), such as Scriptaid, significantly increase the overall cloning efficiency through improvements in the histone acetylation on Histone 4 at lysine 8 (H4K8ac) in a pattern similar to that of the IVF embryos (Zhao et al 2010). Other HDACi, such as m-carboxycinnamic acid bishydroxamide (Song et al 2014), trichostatin A (Li et al 2008) and valproic acid (Kim et al 2011), have also been demonstrated to be beneficial to the in vitro development of porcine SCNT embryos (Miyoshi et al 2010, Zhao et al 2010, Samiec et al 2015.…”

Section: Introductionmentioning

confidence: 99%

BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei

Huang¹,

Zhang²,

Yao³

et al. 2016

Reproduction

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Accumulating evidence suggests that faulty epigenetic reprogramming leads to the abnormal development of cloned embryos and results in the low success rates observed in all mammals produced through somatic cell nuclear transfer (SCNT). The aberrant methylation status of H3K9me and H3K9me2 has been reported in cloned mouse embryos. To explore the role of H3K9me2 and H3K9me in the porcine somatic cell nuclear reprogramming, BIX-01294, known as a specific inhibitor of G9A (histone-lysine methyltransferase of H3K9), was used to treat the nuclear-transferred (NT) oocytes for 14-16 h after activation. The results showed that the developmental competence of porcine SCNT embryos was significantly enhanced both in vitro (blastocyst rate 16.4% vs 23.2%, P!0.05) and in vivo (cloning rate 1.59% vs 2.96%) after 50 nm BIX-01294 treatment. BIX-01294 treatment significantly decreased the levels of H3K9me2 and H3K9me at the 2-and 4-cell stages, which are associated with embryo genetic activation, and increased the transcriptional expression of the pluripotency genes SOX2, NANOG and OCT4 in cloned blastocysts. Furthermore, the histone acetylation levels of H3K9, H4K8 and H4K12 in cloned embryos were decreased after BIX-01294 treatment. However, co-treatment of activated NT oocytes with BIX-01294 and Scriptaid rescued donor nuclear chromatin from decreased histone acetylation of H4K8 that resulted from exposure to BIX-01294 only and consequently improved the preimplantation development of SCNT embryos (blastocyst formation rates of 23.7% vs 21.5%). These results indicated that treatment with BIX-01294 enhanced the developmental competence of porcine SCNT embryos through improvements in epigenetic reprogramming and gene expression.

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Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases theIn VitroDevelopmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos

Cited by 38 publications

References 70 publications

Pharmacologic treatment of donor cells induced to have a Warburg effect‐like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs

Pharmacologic treatment of donor cells induced to have a Warburg effect‐like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei

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