Trichosporon adeninovorans sp. nov., a yeast species utilizing adenine, xanthine, uric acid, putrescine and primary n-alkylamines as the sole source of carbon, nitrogen and energy

Abstract

A new yeast species, Trichosporon adeninovorans, was isolated from soil by the enrichment culture method. Apart from adenine, the strain utilized uric acid, guanine, xanthine, hypoxanthine, 6,8-dihydroxypurine, putrescine, propylamine, butylamine, pentylamine, hexylamine and octylamine as sole source of carbon, nitrogen and energy. The structure of the cell wall of Tr. adeninovorans was ascomycetous. On the subcellular level growth on adenine or uric acid was accompanied with the development of microbodies in the cell. These cell organelles probably were the site of urate oxidase, an enzyme that, after growth on purine substrates, together with allantoinase was present at high activities. Low activities of adenine amidohydrolase and xanthine dehydrogenase were also demonstrated.

Species-specific extracellular antigen production by ascomycetous yeasts, detected by ELISA.

“…Both species have in common the ability to grow with adenine as source of carbon and energy (20). This is a very rare property among yeasts (20,21).…”

The molecular phylogeny of the Ascomycetous anamorphic yeast genus Arxula van der Walt, Smith et Yamada based on the partial sequences of 18S and 26S ribosomal ribonucleic acids.

“…The discrimination between the two species is in the ability to ferment sugars: A, adeninivorans has it but A. terrestris does not (8,12). Considering the calculated number of base differences, the genus Arxula is not so small phylogenetically.…”

“…Yarrowia lipolytica is phylogenetically distant from any other species examined: the species was linked at 9 base differences. (8).…”

Atan1p—an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme

“…Blastobotrys adeninivorans; Kurtzmann and Robnett, 2007). This species belongs among the non-conventional, nonpathogenic yeasts that have unusual biochemical features (Middelhoven et al, 1984(Middelhoven et al, , 1991(Middelhoven et al, , 1992Gienow et al, 1990;Van der Walt et al, 1990). It is able to assimilate and utilize a very broad range of substrates, including tannic acid, purines, starch and phytic acid, as energy and carbon sources (Middelhoven et al, 1984(Middelhoven et al, , 1991(Middelhoven et al, , 1992Gienow et al, 1990;Van der Walt et al, 1990;Wartmann et al, 1995b).…”